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Non-covalent pomegranate (Punica granatum) hydrolyzable tannin-protein complexes modulate antigen uptake, processing and presentation by a T-cell hybridoma line co-cultured with murine peritoneal macrophages.
Int J Food Sci Nutr. 2016 Dec; 67(8):960-8.IJ

Abstract

In this work we characterize the interaction of pomegranate hydrolyzable tannins (HT) with hen egg-white lysozyme (HEL) and determine the effects of non-covalent tannin-protein complexes on macrophage endocytosis, processing and presentation of antigen. We isolated HT from pomegranate and complex to HEL, the resulting non-covalent tannin-protein complex was characterized by gel electrophoresis and MALDI-TOF MS. Finally, cell culture studies and confocal microscopy imaging were conducted on the non-covalent pomegranate HT-HEL protein complexes to evaluate its effect on macrophage antigen uptake, processing and presentation to T-cell hybridomas. Our results indicate that non-covalent pomegranate HT-HEL protein complexes modulate uptake, processing and antigen presentation by mouse peritoneal macrophages. After 4 h of pre-incubation, only trace amounts of IL-2 were detected in the co-cultures treated with HEL alone, whereas a non-covalent pomegranate HT-HEL complex had already reached maximum IL-2 expression. Pomegranate HT may increase rate of endocytose of HEL and subsequent expression of IL-2 by the T-cell hybridomas.

Authors+Show Affiliations

a National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, Edificio Franklin Chang Diaz , San Jose , Costa Rica ;b Department of Animal Sciences, Reed Research Group , University of Wisconsin-Madison , Madison , WI , USA ;c Mass Spectrometry Facility, Department of Chemistry , University of Wisconsin-Madison , Madison , WI , USA.b Department of Animal Sciences, Reed Research Group , University of Wisconsin-Madison , Madison , WI , USA ;b Department of Animal Sciences, Reed Research Group , University of Wisconsin-Madison , Madison , WI , USA ;

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27406472

Citation

Madrigal-Carballo, Sergio, et al. "Non-covalent Pomegranate (Punica Granatum) Hydrolyzable Tannin-protein Complexes Modulate Antigen Uptake, Processing and Presentation By a T-cell Hybridoma Line Co-cultured With Murine Peritoneal Macrophages." International Journal of Food Sciences and Nutrition, vol. 67, no. 8, 2016, pp. 960-8.
Madrigal-Carballo S, Haas L, Vestling M, et al. Non-covalent pomegranate (Punica granatum) hydrolyzable tannin-protein complexes modulate antigen uptake, processing and presentation by a T-cell hybridoma line co-cultured with murine peritoneal macrophages. Int J Food Sci Nutr. 2016;67(8):960-8.
Madrigal-Carballo, S., Haas, L., Vestling, M., Krueger, C. G., & Reed, J. D. (2016). Non-covalent pomegranate (Punica granatum) hydrolyzable tannin-protein complexes modulate antigen uptake, processing and presentation by a T-cell hybridoma line co-cultured with murine peritoneal macrophages. International Journal of Food Sciences and Nutrition, 67(8), 960-8. https://doi.org/10.1080/09637486.2016.1205551
Madrigal-Carballo S, et al. Non-covalent Pomegranate (Punica Granatum) Hydrolyzable Tannin-protein Complexes Modulate Antigen Uptake, Processing and Presentation By a T-cell Hybridoma Line Co-cultured With Murine Peritoneal Macrophages. Int J Food Sci Nutr. 2016;67(8):960-8. PubMed PMID: 27406472.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Non-covalent pomegranate (Punica granatum) hydrolyzable tannin-protein complexes modulate antigen uptake, processing and presentation by a T-cell hybridoma line co-cultured with murine peritoneal macrophages. AU - Madrigal-Carballo,Sergio, AU - Haas,Linda, AU - Vestling,Martha, AU - Krueger,Christian G, AU - Reed,Jess D, Y1 - 2016/07/12/ PY - 2016/7/14/entrez PY - 2016/7/14/pubmed PY - 2018/2/6/medline KW - Tannins KW - polyphenols KW - pomegranate juice KW - proteins SP - 960 EP - 8 JF - International journal of food sciences and nutrition JO - Int J Food Sci Nutr VL - 67 IS - 8 N2 - In this work we characterize the interaction of pomegranate hydrolyzable tannins (HT) with hen egg-white lysozyme (HEL) and determine the effects of non-covalent tannin-protein complexes on macrophage endocytosis, processing and presentation of antigen. We isolated HT from pomegranate and complex to HEL, the resulting non-covalent tannin-protein complex was characterized by gel electrophoresis and MALDI-TOF MS. Finally, cell culture studies and confocal microscopy imaging were conducted on the non-covalent pomegranate HT-HEL protein complexes to evaluate its effect on macrophage antigen uptake, processing and presentation to T-cell hybridomas. Our results indicate that non-covalent pomegranate HT-HEL protein complexes modulate uptake, processing and antigen presentation by mouse peritoneal macrophages. After 4 h of pre-incubation, only trace amounts of IL-2 were detected in the co-cultures treated with HEL alone, whereas a non-covalent pomegranate HT-HEL complex had already reached maximum IL-2 expression. Pomegranate HT may increase rate of endocytose of HEL and subsequent expression of IL-2 by the T-cell hybridomas. SN - 1465-3478 UR - https://www.unboundmedicine.com/medline/citation/27406472/Non_covalent_pomegranate__Punica_granatum__hydrolyzable_tannin_protein_complexes_modulate_antigen_uptake_processing_and_presentation_by_a_T_cell_hybridoma_line_co_cultured_with_murine_peritoneal_macrophages_ L2 - http://www.tandfonline.com/doi/full/10.1080/09637486.2016.1205551 DB - PRIME DP - Unbound Medicine ER -