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One-pot polyglycidol nanogels via liposome master templates for dual drug delivery.
J Control Release. 2016 12 28; 244(Pt B):366-374.JC

Abstract

Polyglycidol-based nanohydrogels (nHGs) have been prepared by optimizing the use of liposome master templates resulting in a high-yielding and more practical one-pot process to provide materials capable of carrying drugs of adverse chemical nature. The nanogels prepared with the one-pot method showed favorable kinetics for the release of either Nile Red (NR) or lysozyme (LYS), loaded with gel precursors such as semi-branched poly(glycidol allylglycidyl ether), PEG dithiol (1KDa), a free radical initiator and liposomal lipids at the liposome formation step. This process is superior to a comparable step-wise traditional approach and circumvents loading of the gel precursors with the hydrophilic drug into preformed liposome templates. A thiol-ene crosslinking reaction accomplishes the formation of the nanonetwork resulting in nHGs prepared in the traditional step-wise (nHG-SW) approach and the one-pot (nHG-OP) process. Both nanogel networks were characterized in terms of particle size and zeta (ζ) potential with average values of 148nm±39nm and -25.9mV±9.2 for the nHG-SW and 132nm±32 and -23.1mV±9.7 for the nHG-OPs. Loading efficiency for both of the nanogels with NR was determined by spectrophotometry to be 28% (nHP-SW) and 31% (nHP-OP). The LYS loading was based on the target loading of 10μg/mg for both nanogels found to be 84% and 86% for the nHG-SW and nHP-OP, respectively. As proof of concept for combination drug delivery, the in vitro release of both drug mimics, NR and LYS, were monitored under physiologically relevant conditions by an optimized dialysis method. The implementation of the multi-functional and semi-branched polyglycidol is recognized as the main contributor for the observed highly controlled release of proteins that are otherwise rapidly released from common PEG-based nanogel networks. Furthermore, the one-pot process led to be the most favorable drug delivery system based on the release kinetics pointing to a denser polymer network.

Authors+Show Affiliations

Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt Institute of Chemical Biology, Vanderbilt University, 7665 Stevenson Center, Nashville, TN 37235, United States.Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt Institute of Chemical Biology, Vanderbilt University, 7665 Stevenson Center, Nashville, TN 37235, United States.Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37212, United States.Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt Institute of Chemical Biology, Vanderbilt University, 7665 Stevenson Center, Nashville, TN 37235, United States.Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt Institute of Chemical Biology, Vanderbilt University, 7665 Stevenson Center, Nashville, TN 37235, United States; Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37212, United States. Electronic address: eva.harth@vanderbilt.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27411978

Citation

Lockhart, Jacob N., et al. "One-pot Polyglycidol Nanogels Via Liposome Master Templates for Dual Drug Delivery." Journal of Controlled Release : Official Journal of the Controlled Release Society, vol. 244, no. Pt B, 2016, pp. 366-374.
Lockhart JN, Beezer DB, Stevens DM, et al. One-pot polyglycidol nanogels via liposome master templates for dual drug delivery. J Control Release. 2016;244(Pt B):366-374.
Lockhart, J. N., Beezer, D. B., Stevens, D. M., Spears, B. R., & Harth, E. (2016). One-pot polyglycidol nanogels via liposome master templates for dual drug delivery. Journal of Controlled Release : Official Journal of the Controlled Release Society, 244(Pt B), 366-374. https://doi.org/10.1016/j.jconrel.2016.07.013
Lockhart JN, et al. One-pot Polyglycidol Nanogels Via Liposome Master Templates for Dual Drug Delivery. J Control Release. 2016 12 28;244(Pt B):366-374. PubMed PMID: 27411978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - One-pot polyglycidol nanogels via liposome master templates for dual drug delivery. AU - Lockhart,Jacob N, AU - Beezer,Dain B, AU - Stevens,David M, AU - Spears,Benjamin R, AU - Harth,Eva, Y1 - 2016/07/10/ PY - 2016/05/26/received PY - 2016/06/23/revised PY - 2016/07/07/accepted PY - 2016/7/15/pubmed PY - 2018/1/4/medline PY - 2016/7/15/entrez KW - Chemotherapy KW - Combination therapy KW - Liposome KW - Lysozyme KW - Nano-hydrogel KW - Nanoparticle drug delivery KW - Nile red SP - 366 EP - 374 JF - Journal of controlled release : official journal of the Controlled Release Society JO - J Control Release VL - 244 IS - Pt B N2 - Polyglycidol-based nanohydrogels (nHGs) have been prepared by optimizing the use of liposome master templates resulting in a high-yielding and more practical one-pot process to provide materials capable of carrying drugs of adverse chemical nature. The nanogels prepared with the one-pot method showed favorable kinetics for the release of either Nile Red (NR) or lysozyme (LYS), loaded with gel precursors such as semi-branched poly(glycidol allylglycidyl ether), PEG dithiol (1KDa), a free radical initiator and liposomal lipids at the liposome formation step. This process is superior to a comparable step-wise traditional approach and circumvents loading of the gel precursors with the hydrophilic drug into preformed liposome templates. A thiol-ene crosslinking reaction accomplishes the formation of the nanonetwork resulting in nHGs prepared in the traditional step-wise (nHG-SW) approach and the one-pot (nHG-OP) process. Both nanogel networks were characterized in terms of particle size and zeta (ζ) potential with average values of 148nm±39nm and -25.9mV±9.2 for the nHG-SW and 132nm±32 and -23.1mV±9.7 for the nHG-OPs. Loading efficiency for both of the nanogels with NR was determined by spectrophotometry to be 28% (nHP-SW) and 31% (nHP-OP). The LYS loading was based on the target loading of 10μg/mg for both nanogels found to be 84% and 86% for the nHG-SW and nHP-OP, respectively. As proof of concept for combination drug delivery, the in vitro release of both drug mimics, NR and LYS, were monitored under physiologically relevant conditions by an optimized dialysis method. The implementation of the multi-functional and semi-branched polyglycidol is recognized as the main contributor for the observed highly controlled release of proteins that are otherwise rapidly released from common PEG-based nanogel networks. Furthermore, the one-pot process led to be the most favorable drug delivery system based on the release kinetics pointing to a denser polymer network. SN - 1873-4995 UR - https://www.unboundmedicine.com/medline/citation/27411978/One_pot_polyglycidol_nanogels_via_liposome_master_templates_for_dual_drug_delivery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-3659(16)30447-3 DB - PRIME DP - Unbound Medicine ER -