Tags

Type your tag names separated by a space and hit enter

A fatty acyl desaturase (fads2) with dual Δ6 and Δ5 activities from the freshwater carnivorous striped snakehead Channa striata.

Abstract

There is a lack of understanding on how the environment and trophic niche affect the capability of long-chain polyunsaturated fatty acids (LC-PUFA) in freshwater carnivorous teleost. In this present study, we isolated and functionally characterised a fatty acyl desaturase (Fads) from the striped snakehead Channa striata. Sequence comparison and phylogenetic analysis suggested a Fads2 protein that is closely related to previously characterised Fads2 proteins from freshwater carnivorous and marine herbivorous fish species. We further demonstrated the capacity of Δ6 and Δ5 desaturation activities for this particular desaturase, with highest activities towards the conversion of omega-3 (n-3) polyunsaturated fatty acids (PUFA). Low Δ4 desaturation activity was also detected, although the significance of this at a physiological level remains to be studied. The expression of this striped snakehead Δ6/Δ5 fads2 gene was highest in brain, followed by liver and intestine. In liver, diet fortified with high LC-PUFA concentration impeded the expression of Δ6/Δ5 fads2 gene compared to vegetable oil (VO) based diets. The discovery of Δ6/Δ5 Fads2 desaturase here complements the previous discovery of a Δ4 Fads2 desaturase and an Elovl5 elongase, lending proof to the existence of all the required enzymatic machinery to biosynthesise LC-PUFA from C18 PUFA in a freshwater carnivorous species.

Authors+Show Affiliations

Centre for Chemical Biology, Universiti Sains Malaysia, Sains@USM, Blok B No. 10, Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia.Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.Centre for Chemical Biology, Universiti Sains Malaysia, Sains@USM, Blok B No. 10, Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia; Centre for Marine and Coastal Studies, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: alex@usm.my.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27421235

Citation

Kuah, Meng-Kiat, et al. "A Fatty Acyl Desaturase (fads2) With Dual Δ6 and Δ5 Activities From the Freshwater Carnivorous Striped Snakehead Channa Striata." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 201, 2016, pp. 146-155.
Kuah MK, Jaya-Ram A, Shu-Chien AC. A fatty acyl desaturase (fads2) with dual Δ6 and Δ5 activities from the freshwater carnivorous striped snakehead Channa striata. Comp Biochem Physiol, Part A Mol Integr Physiol. 2016;201:146-155.
Kuah, M. K., Jaya-Ram, A., & Shu-Chien, A. C. (2016). A fatty acyl desaturase (fads2) with dual Δ6 and Δ5 activities from the freshwater carnivorous striped snakehead Channa striata. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 201, pp. 146-155. doi:10.1016/j.cbpa.2016.07.007.
Kuah MK, Jaya-Ram A, Shu-Chien AC. A Fatty Acyl Desaturase (fads2) With Dual Δ6 and Δ5 Activities From the Freshwater Carnivorous Striped Snakehead Channa Striata. Comp Biochem Physiol, Part A Mol Integr Physiol. 2016;201:146-155. PubMed PMID: 27421235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fatty acyl desaturase (fads2) with dual Δ6 and Δ5 activities from the freshwater carnivorous striped snakehead Channa striata. AU - Kuah,Meng-Kiat, AU - Jaya-Ram,Annette, AU - Shu-Chien,Alexander Chong, Y1 - 2016/07/12/ PY - 2016/05/12/received PY - 2016/07/05/revised PY - 2016/07/08/accepted PY - 2016/7/17/entrez PY - 2016/7/18/pubmed PY - 2017/7/25/medline SP - 146 EP - 155 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 201 N2 - There is a lack of understanding on how the environment and trophic niche affect the capability of long-chain polyunsaturated fatty acids (LC-PUFA) in freshwater carnivorous teleost. In this present study, we isolated and functionally characterised a fatty acyl desaturase (Fads) from the striped snakehead Channa striata. Sequence comparison and phylogenetic analysis suggested a Fads2 protein that is closely related to previously characterised Fads2 proteins from freshwater carnivorous and marine herbivorous fish species. We further demonstrated the capacity of Δ6 and Δ5 desaturation activities for this particular desaturase, with highest activities towards the conversion of omega-3 (n-3) polyunsaturated fatty acids (PUFA). Low Δ4 desaturation activity was also detected, although the significance of this at a physiological level remains to be studied. The expression of this striped snakehead Δ6/Δ5 fads2 gene was highest in brain, followed by liver and intestine. In liver, diet fortified with high LC-PUFA concentration impeded the expression of Δ6/Δ5 fads2 gene compared to vegetable oil (VO) based diets. The discovery of Δ6/Δ5 Fads2 desaturase here complements the previous discovery of a Δ4 Fads2 desaturase and an Elovl5 elongase, lending proof to the existence of all the required enzymatic machinery to biosynthesise LC-PUFA from C18 PUFA in a freshwater carnivorous species. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/27421235/A_fatty_acyl_desaturase__fads2__with_dual_Δ6_and_Δ5_activities_from_the_freshwater_carnivorous_striped_snakehead_Channa_striata_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(16)30152-0 DB - PRIME DP - Unbound Medicine ER -