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Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat.
Theor Appl Genet. 2016 Oct; 129(10):1975-84.TA

Abstract

KEY MESSAGE

Powdery mildew resistance gene Pm55 was physically mapped to chromosome arm 5VS FL 0.60-0.80 of Dasypyrum villosum . Pm55 is present in T5VS·5AL and T5VS·5DL translocations, which should be valuable resources for wheat improvement. Powdery mildew caused by Blumeria graminis f. sp. tritici is a major wheat disease worldwide. Exploiting novel genes effective against powdery mildew from wild relatives of wheat is a promising strategy for controlling this disease. To identify novel resistance genes for powdery mildew from Dasypyrum villosum, a wild wheat relative, we evaluated a set of Chinese Spring-D. villosum disomic addition and whole-arm translocation lines for reactions to powdery mildew. Based on the evaluation data, we concluded that the D. villosum chromosome 5V controls post-seedling resistance to powdery mildew. Subsequently, three introgression lines were developed and confirmed by molecular and cytogenetic analysis following ionizing radiation of the pollen of a Chinese Spring-D. villosum 5V disomic addition line. A homozygous T5VS·5AL translocation line (NAU421) with good plant vigor and full fertility was further characterized using sequential genomic in situ hybridization, C-banding, and EST-STS marker analysis. A dominant gene permanently named Pm55 was located in chromosome bin 5VS 0.60-0.80 based on the responses to powdery mildew of all wheat-D. villosum 5V introgression lines evaluated at both seeding and adult stages. This study demonstrated that Pm55 conferred growth-stage and tissue-specific dependent resistance; therefore, it provides a novel resistance type for powdery mildew. The T5VS·5AL translocation line with additional softness loci Dina/Dinb of D. villosum provides a possibility of extending the range of grain textures to a super-soft category. Accordingly, this stock is a new source of resistance to powdery mildew and may be useful in both resistance mechanism studies and soft wheat improvement.

Authors+Show Affiliations

College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China. zrq@njau.edu.cn.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.International Maize and Wheat Improvement Center (CIMMYT), 06600, Mexico, DF, Mexico.College of Agronomy, Nanjing Agricultural University/National Key Laboratory of Crop Genetics and Germplasm Enhancement/JCIC-MCP, Nanjing, 210095, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27422445

Citation

Zhang, Ruiqi, et al. "Pm55, a Developmental-stage and Tissue-specific Powdery Mildew Resistance Gene Introgressed From Dasypyrum Villosum Into Common Wheat." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 10, 2016, pp. 1975-84.
Zhang R, Sun B, Chen J, et al. Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. Theor Appl Genet. 2016;129(10):1975-84.
Zhang, R., Sun, B., Chen, J., Cao, A., Xing, L., Feng, Y., Lan, C., & Chen, P. (2016). Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(10), 1975-84. https://doi.org/10.1007/s00122-016-2753-8
Zhang R, et al. Pm55, a Developmental-stage and Tissue-specific Powdery Mildew Resistance Gene Introgressed From Dasypyrum Villosum Into Common Wheat. Theor Appl Genet. 2016;129(10):1975-84. PubMed PMID: 27422445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. AU - Zhang,Ruiqi, AU - Sun,Bingxiao, AU - Chen,Juan, AU - Cao,Aizhong, AU - Xing,Liping, AU - Feng,Yigao, AU - Lan,Caixia, AU - Chen,Peidu, Y1 - 2016/07/15/ PY - 2016/04/20/received PY - 2016/07/09/accepted PY - 2016/7/17/entrez PY - 2016/7/17/pubmed PY - 2017/1/6/medline SP - 1975 EP - 84 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 129 IS - 10 N2 - KEY MESSAGE: Powdery mildew resistance gene Pm55 was physically mapped to chromosome arm 5VS FL 0.60-0.80 of Dasypyrum villosum . Pm55 is present in T5VS·5AL and T5VS·5DL translocations, which should be valuable resources for wheat improvement. Powdery mildew caused by Blumeria graminis f. sp. tritici is a major wheat disease worldwide. Exploiting novel genes effective against powdery mildew from wild relatives of wheat is a promising strategy for controlling this disease. To identify novel resistance genes for powdery mildew from Dasypyrum villosum, a wild wheat relative, we evaluated a set of Chinese Spring-D. villosum disomic addition and whole-arm translocation lines for reactions to powdery mildew. Based on the evaluation data, we concluded that the D. villosum chromosome 5V controls post-seedling resistance to powdery mildew. Subsequently, three introgression lines were developed and confirmed by molecular and cytogenetic analysis following ionizing radiation of the pollen of a Chinese Spring-D. villosum 5V disomic addition line. A homozygous T5VS·5AL translocation line (NAU421) with good plant vigor and full fertility was further characterized using sequential genomic in situ hybridization, C-banding, and EST-STS marker analysis. A dominant gene permanently named Pm55 was located in chromosome bin 5VS 0.60-0.80 based on the responses to powdery mildew of all wheat-D. villosum 5V introgression lines evaluated at both seeding and adult stages. This study demonstrated that Pm55 conferred growth-stage and tissue-specific dependent resistance; therefore, it provides a novel resistance type for powdery mildew. The T5VS·5AL translocation line with additional softness loci Dina/Dinb of D. villosum provides a possibility of extending the range of grain textures to a super-soft category. Accordingly, this stock is a new source of resistance to powdery mildew and may be useful in both resistance mechanism studies and soft wheat improvement. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27422445/Pm55_a_developmental_stage_and_tissue_specific_powdery_mildew_resistance_gene_introgressed_from_Dasypyrum_villosum_into_common_wheat_ L2 - https://dx.doi.org/10.1007/s00122-016-2753-8 DB - PRIME DP - Unbound Medicine ER -