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Involvement of vacuolar processing enzyme SlVPE5 in post-transcriptional process of invertase in sucrose accumulation in tomato.
Plant Physiol Biochem. 2016 Nov; 108:71-78.PP

Abstract

Enhancing the flavor of fruits plays a fundamental role in improving fruit quality, and volatile compositions as well as acid and sugar accumulation are significant factors that have an impact on the acceptability of sensory responses by human beings. Vacuoles in plants not only function as cell compartments that store amino acids, sugars and other metabolites but also act as lytic organelles where vacuolar proteins are post-translationally processed into mature forms or degraded by the action of vacuolar processing enzyme (VPE). We have previously characterized VPE genes (SlVPE1-5) during fruit development in tomato and discovered that the VPE enzyme activity negatively interfered with sugar accumulation in mature fruits. Comparative proteomic analysis demonstrated that acid invertase was one of the molecular targets of SlVPE5, which is involved in the hydrolysis of sucrose. This study also showed that decreased VPE enzyme activity due to suppression of SlVPE5 by RNAi strategy (RNAi-SlVPE5) accompanied with decreased enzyme activity of acid invertase. Further, we identified the enzyme activity of acid invertase was not well correlated with mRNA levels in the RNAi-SlVPE5 line. These results suggest that SlVPE5 regulates post-transcriptional processing through de novo synthesis of the acid invertase protein to suppress enzyme activity, thereby eventually ensuring sucrose hydrolysis.

Authors+Show Affiliations

Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. Electronic address: ezura@gene.tsukuba.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27423072

Citation

Wang, Ning, et al. "Involvement of Vacuolar Processing Enzyme SlVPE5 in Post-transcriptional Process of Invertase in Sucrose Accumulation in Tomato." Plant Physiology and Biochemistry : PPB, vol. 108, 2016, pp. 71-78.
Wang N, Duhita N, Ariizumi T, et al. Involvement of vacuolar processing enzyme SlVPE5 in post-transcriptional process of invertase in sucrose accumulation in tomato. Plant Physiol Biochem. 2016;108:71-78.
Wang, N., Duhita, N., Ariizumi, T., & Ezura, H. (2016). Involvement of vacuolar processing enzyme SlVPE5 in post-transcriptional process of invertase in sucrose accumulation in tomato. Plant Physiology and Biochemistry : PPB, 108, 71-78. https://doi.org/10.1016/j.plaphy.2016.06.037
Wang N, et al. Involvement of Vacuolar Processing Enzyme SlVPE5 in Post-transcriptional Process of Invertase in Sucrose Accumulation in Tomato. Plant Physiol Biochem. 2016;108:71-78. PubMed PMID: 27423072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of vacuolar processing enzyme SlVPE5 in post-transcriptional process of invertase in sucrose accumulation in tomato. AU - Wang,Ning, AU - Duhita,Narendra, AU - Ariizumi,Toru, AU - Ezura,Hiroshi, Y1 - 2016/06/29/ PY - 2016/05/05/received PY - 2016/06/26/revised PY - 2016/06/28/accepted PY - 2016/10/21/pubmed PY - 2017/3/31/medline PY - 2016/7/17/entrez KW - Acid invertase KW - Fruit KW - Post-transcriptional regulation KW - RNA interference KW - Vacuolar processing enzyme SP - 71 EP - 78 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 108 N2 - Enhancing the flavor of fruits plays a fundamental role in improving fruit quality, and volatile compositions as well as acid and sugar accumulation are significant factors that have an impact on the acceptability of sensory responses by human beings. Vacuoles in plants not only function as cell compartments that store amino acids, sugars and other metabolites but also act as lytic organelles where vacuolar proteins are post-translationally processed into mature forms or degraded by the action of vacuolar processing enzyme (VPE). We have previously characterized VPE genes (SlVPE1-5) during fruit development in tomato and discovered that the VPE enzyme activity negatively interfered with sugar accumulation in mature fruits. Comparative proteomic analysis demonstrated that acid invertase was one of the molecular targets of SlVPE5, which is involved in the hydrolysis of sucrose. This study also showed that decreased VPE enzyme activity due to suppression of SlVPE5 by RNAi strategy (RNAi-SlVPE5) accompanied with decreased enzyme activity of acid invertase. Further, we identified the enzyme activity of acid invertase was not well correlated with mRNA levels in the RNAi-SlVPE5 line. These results suggest that SlVPE5 regulates post-transcriptional processing through de novo synthesis of the acid invertase protein to suppress enzyme activity, thereby eventually ensuring sucrose hydrolysis. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27423072/Involvement_of_vacuolar_processing_enzyme_SlVPE5_in_post_transcriptional_process_of_invertase_in_sucrose_accumulation_in_tomato_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30267-4 DB - PRIME DP - Unbound Medicine ER -