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Serotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattle.
J Appl Microbiol. 2016 Oct; 121(4):1130-43.JA

Abstract

AIM

The occurrence of virulence markers, serotypes and invasive ability were investigated in Shiga toxin-producing Escherichia coli (STEC) isolated from faecal samples of healthy dairy cattle at Rio de Janeiro State, Brazil.

METHODS AND RESULTS

From 1562 stx-positive faecal samples, 105 STEC strains were isolated by immuno-magnetic separation (IMS) or plating onto MacConkey agar (MC) followed by colony hybridisation. Fifty (47·6%) strains belonged to nine serotypes (O8:H19, O22:H8, O22:H16, O74:H42, O113:H21, O141:H21, O157:H7, O171:H2 and ONT:H21). The prevalent serotypes were O157:H7 (12·4%), O113:H21 (6·7%) and O8:H19 (5·7%). Virulence genes were identified by polymerase chain reaction (PCR). E-hlyA (77·1%) was the more prevalent virulence marker, followed by espP (64·8%), saa (39%), eae (24·8%) and astA (21·9%). All O157:H7 strains carried the γ (gamma) variant of the locus of enterocyte effacement (LEE) genes and the stx2c gene, while the stx1/stx2 genotype prevailed among the eae-negative strains. None of the eae-positive STEC produced the localized adherence (LA) phenotype in HEp-2 or Caco-2 cells. However, intimate attachment (judged by the fluorescent actin staining test) was detected in some eae-positive strains, both in HEp-2 (23·1%) and in Caco-2 cells (11·5%). Most strains (87·5%) showed 'peripheral association' (PA) adherence phenotype to undifferentiated Caco-2 cells. Twenty-five (92·6%) of 27 strains invaded Caco-2 cells. The highest average value of invasion (9·6%) was observed among the eae-negative bovine strains from serotypes described in human disease.

CONCLUSION

Healthy dairy cattle is a reservoir of STEC carrying virulence genes and properties associated with human disease.

SIGNIFICANCE AND IMPACT OF THE STUDY

Although reports of human disease associated with STEC are scarce in Brazil, the colonization of the animal reservoir by potentially pathogenic strains offers a significant risk to our population.

Authors+Show Affiliations

Departamento de Bromatologia, Universidade Federal Fluminense, Niterói, Brasil. aliceg@id.uff.br.Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brasil.Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brasil.Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil.Centro Estadual de Pesquisa em Sanidade Animal, Pesagro-Rio, Niterói, Brasil.Centro Estadual de Pesquisa em Sanidade Animal, Pesagro-Rio, Niterói, Brasil.Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brasil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27426967

Citation

Gonzalez, A G M., et al. "Serotypes, Virulence Markers and Cell Invasion Ability of Shiga Toxin-producing Escherichia Coli Strains Isolated From Healthy Dairy Cattle." Journal of Applied Microbiology, vol. 121, no. 4, 2016, pp. 1130-43.
Gonzalez AG, Cerqueira AM, Guth BE, et al. Serotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattle. J Appl Microbiol. 2016;121(4):1130-43.
Gonzalez, A. G., Cerqueira, A. M., Guth, B. E., Coutinho, C. A., Liberal, M. H., Souza, R. M., & Andrade, J. R. (2016). Serotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattle. Journal of Applied Microbiology, 121(4), 1130-43. https://doi.org/10.1111/jam.13230
Gonzalez AG, et al. Serotypes, Virulence Markers and Cell Invasion Ability of Shiga Toxin-producing Escherichia Coli Strains Isolated From Healthy Dairy Cattle. J Appl Microbiol. 2016;121(4):1130-43. PubMed PMID: 27426967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serotypes, virulence markers and cell invasion ability of Shiga toxin-producing Escherichia coli strains isolated from healthy dairy cattle. AU - Gonzalez,A G M, AU - Cerqueira,A M F, AU - Guth,B E C, AU - Coutinho,C A, AU - Liberal,M H T, AU - Souza,R M, AU - Andrade,J R C, Y1 - 2016/08/25/ PY - 2016/03/05/received PY - 2016/06/10/revised PY - 2016/06/20/accepted PY - 2016/7/19/entrez PY - 2016/7/19/pubmed PY - 2017/5/10/medline KW - Escherichia coli O157:H7 KW - Shiga toxin-producing Escherichia coli KW - cattle KW - cell invasion KW - serotypes KW - virulence markers SP - 1130 EP - 43 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 121 IS - 4 N2 - AIM: The occurrence of virulence markers, serotypes and invasive ability were investigated in Shiga toxin-producing Escherichia coli (STEC) isolated from faecal samples of healthy dairy cattle at Rio de Janeiro State, Brazil. METHODS AND RESULTS: From 1562 stx-positive faecal samples, 105 STEC strains were isolated by immuno-magnetic separation (IMS) or plating onto MacConkey agar (MC) followed by colony hybridisation. Fifty (47·6%) strains belonged to nine serotypes (O8:H19, O22:H8, O22:H16, O74:H42, O113:H21, O141:H21, O157:H7, O171:H2 and ONT:H21). The prevalent serotypes were O157:H7 (12·4%), O113:H21 (6·7%) and O8:H19 (5·7%). Virulence genes were identified by polymerase chain reaction (PCR). E-hlyA (77·1%) was the more prevalent virulence marker, followed by espP (64·8%), saa (39%), eae (24·8%) and astA (21·9%). All O157:H7 strains carried the γ (gamma) variant of the locus of enterocyte effacement (LEE) genes and the stx2c gene, while the stx1/stx2 genotype prevailed among the eae-negative strains. None of the eae-positive STEC produced the localized adherence (LA) phenotype in HEp-2 or Caco-2 cells. However, intimate attachment (judged by the fluorescent actin staining test) was detected in some eae-positive strains, both in HEp-2 (23·1%) and in Caco-2 cells (11·5%). Most strains (87·5%) showed 'peripheral association' (PA) adherence phenotype to undifferentiated Caco-2 cells. Twenty-five (92·6%) of 27 strains invaded Caco-2 cells. The highest average value of invasion (9·6%) was observed among the eae-negative bovine strains from serotypes described in human disease. CONCLUSION: Healthy dairy cattle is a reservoir of STEC carrying virulence genes and properties associated with human disease. SIGNIFICANCE AND IMPACT OF THE STUDY: Although reports of human disease associated with STEC are scarce in Brazil, the colonization of the animal reservoir by potentially pathogenic strains offers a significant risk to our population. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/27426967/Serotypes_virulence_markers_and_cell_invasion_ability_of_Shiga_toxin_producing_Escherichia_coli_strains_isolated_from_healthy_dairy_cattle_ L2 - https://doi.org/10.1111/jam.13230 DB - PRIME DP - Unbound Medicine ER -