Tags

Type your tag names separated by a space and hit enter

Protective effect of ellagic acid against cyclosporine A-induced histopathological, ultrastructural changes, oxidative stress, and cytogenotoxicity in albino rats.

Abstract

Cyclosporine A (CsA) is an immunosuppressor agent, which is most frequently used in transplant surgeries and in the treatment of autoimmune diseases. This study was undertaken to investigate the protective effects of ellagic acid (EA) against CsA-induced testicular histopathology and ultrastructure changes, oxidative stress, and cytogenotoxicity in male albino rats. Rats were divided into six groups; the first group was used as a control, the second group received a subcutaneous injection of slightly alkaline solution, the third group received olive oil orally, the fourth group was injected subcutaneously with EA at a dose of 10 mg/kg b. wt./day, the fifth group was treated with CsA as oral solution at a dose of 15 mg/kg b. wt for 30 days, and the sixth group was treated with CsA simultaneously with EA. Treatment with EA simultaneously with CsA resulted in significant protection. The positive control animals taking CsA alone showed marked histopathological, ultrastructure, and genetic manifestations accompanied by an elevated content of lipid peroxidation and marked reduction of catalase (CAT), peroxidase (Px) activity, and glutathione concentration in the homogenate of testis tissues. The toxic side effects in testis and bone marrow tissues were greatly ablated with a significant reduction in lipid peroxidation level and elevation in CAT and Px activities and glutathione concentration when using EA. Thus, EA may be used in combination with CsA to improve the histopathological, oxidative stress, and cytogenotoxicity parameters of testicular toxicity induced by CsA due to its antioxidant effects.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    a Department of Zoology, Faculty of Science , Beni-Suef University , Beni-Suef , Egypt.

    ,

    a Department of Zoology, Faculty of Science , Beni-Suef University , Beni-Suef , Egypt.

    ,

    a Department of Zoology, Faculty of Science , Beni-Suef University , Beni-Suef , Egypt.

    a Department of Zoology, Faculty of Science , Beni-Suef University , Beni-Suef , Egypt.

    Source

    MeSH

    Animals
    Antioxidants
    Cyclosporine
    Ellagic Acid
    Immunosuppressive Agents
    Male
    Microscopy, Electron, Transmission
    Oxidative Stress
    Rats
    Testis

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    27430433

    Citation

    Abdul-Hamid, Manal, et al. "Protective Effect of Ellagic Acid Against Cyclosporine A-induced Histopathological, Ultrastructural Changes, Oxidative Stress, and Cytogenotoxicity in Albino Rats." Ultrastructural Pathology, vol. 40, no. 4, 2016, pp. 205-21.
    Abdul-Hamid M, Abdella EM, Galaly SR, et al. Protective effect of ellagic acid against cyclosporine A-induced histopathological, ultrastructural changes, oxidative stress, and cytogenotoxicity in albino rats. Ultrastruct Pathol. 2016;40(4):205-21.
    Abdul-Hamid, M., Abdella, E. M., Galaly, S. R., & Ahmed, R. H. (2016). Protective effect of ellagic acid against cyclosporine A-induced histopathological, ultrastructural changes, oxidative stress, and cytogenotoxicity in albino rats. Ultrastructural Pathology, 40(4), pp. 205-21. doi:10.1080/01913123.2016.1203854.
    Abdul-Hamid M, et al. Protective Effect of Ellagic Acid Against Cyclosporine A-induced Histopathological, Ultrastructural Changes, Oxidative Stress, and Cytogenotoxicity in Albino Rats. Ultrastruct Pathol. 2016;40(4):205-21. PubMed PMID: 27430433.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Protective effect of ellagic acid against cyclosporine A-induced histopathological, ultrastructural changes, oxidative stress, and cytogenotoxicity in albino rats. AU - Abdul-Hamid,Manal, AU - Abdella,Ehab M, AU - Galaly,Sanaa R, AU - Ahmed,Rania H, Y1 - 2016/07/18/ PY - 2016/7/20/entrez PY - 2016/7/20/pubmed PY - 2017/3/28/medline KW - Cyclosporine A KW - chromosomal aberrations KW - ellagic acid KW - histopathology and ultrastructure KW - oxidative stress SP - 205 EP - 21 JF - Ultrastructural pathology JO - Ultrastruct Pathol VL - 40 IS - 4 N2 - Cyclosporine A (CsA) is an immunosuppressor agent, which is most frequently used in transplant surgeries and in the treatment of autoimmune diseases. This study was undertaken to investigate the protective effects of ellagic acid (EA) against CsA-induced testicular histopathology and ultrastructure changes, oxidative stress, and cytogenotoxicity in male albino rats. Rats were divided into six groups; the first group was used as a control, the second group received a subcutaneous injection of slightly alkaline solution, the third group received olive oil orally, the fourth group was injected subcutaneously with EA at a dose of 10 mg/kg b. wt./day, the fifth group was treated with CsA as oral solution at a dose of 15 mg/kg b. wt for 30 days, and the sixth group was treated with CsA simultaneously with EA. Treatment with EA simultaneously with CsA resulted in significant protection. The positive control animals taking CsA alone showed marked histopathological, ultrastructure, and genetic manifestations accompanied by an elevated content of lipid peroxidation and marked reduction of catalase (CAT), peroxidase (Px) activity, and glutathione concentration in the homogenate of testis tissues. The toxic side effects in testis and bone marrow tissues were greatly ablated with a significant reduction in lipid peroxidation level and elevation in CAT and Px activities and glutathione concentration when using EA. Thus, EA may be used in combination with CsA to improve the histopathological, oxidative stress, and cytogenotoxicity parameters of testicular toxicity induced by CsA due to its antioxidant effects. SN - 1521-0758 UR - https://www.unboundmedicine.com/medline/citation/27430433/Protective_effect_of_ellagic_acid_against_cyclosporine_A_induced_histopathological_ultrastructural_changes_oxidative_stress_and_cytogenotoxicity_in_albino_rats_ L2 - http://www.tandfonline.com/doi/full/10.1080/01913123.2016.1203854 DB - PRIME DP - Unbound Medicine ER -