Tags

Type your tag names separated by a space and hit enter

Melatonin and nitric oxide modulate glutathione content and glutathione reductase activity in sunflower seedling cotyledons accompanying salt stress.
Nitric Oxide. 2016 09 30; 59:42-53.NO

Abstract

The present findings demonstrate significant modulation of total glutathione content, reduced glutathione (GSH) content, oxidized glutathione (GSSG) content, GSH/GSSG ratio and glutathione reductase (GR; EC 1.6.4.2) activity in dark-grown seedling cotyledons in response to salt-stress (120 mM NaCl) in sunflower (Helianthus annuus L.) seedlings. A differential spatial distribution of GR activity (monitored by confocal laser scanning microscopic (CLSM) imaging) is also evident. Melatonin and nitric oxide (NO) differentially ameliorate salt stress effect by modulating GR activity and GSH content in seedling cotyledons. Total glutathione content (GSH + GSSG) exhibit a seedling age-dependent increase in the cotyledons, more so in salt-stressed conditions and when subjected to melatonin treatment. Seedlings raised in presence of 15 μM of melatonin exhibit significant increase in GR activity in cotyledon homogenates (10,000 g supernatant) coinciding with significant increase in GSH content. GSSG content and GSH/GSSG ratio also increased due to melatonin treatment. A correlation is thus evident in NaCl-sensitized modulation of GSH content and GR activity by melatonin. GSH content is down regulated by NO provided as 250 μM of sodium nitroprusside (SNP) although total glutathione content remained in similar range. A reversal of response (enhanced total glutathione accumulation) by NO scavenger (cPTIO) highlights the critical role of NO in modulating glutathione homeostasis. SNP lowers the activity of hydroxyindole-O-methyltransferase (HIOMT) - a regulatory enzyme in melatonin biosynthesis in control seedlings whereas its activity is upregulated in salt-stressed seedling cotyledons. Melatonin content of seedling cotyledons is also modulated by NO. NO and melatonin thus seem to modulate GR activity and GSH content during seedling growth under salt stress.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Kaur H
Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi, Delhi, 110007, India. Electronic address: harmeet.ahden@gmail.com.
Bhatla SC
Laboratory of Plant Physiology and Biochemistry, Department of Botany, University of Delhi, Delhi, 110007, India. Electronic address: bhatlasc@gmail.com.

MeSH

Acetylserotonin O-MethyltransferaseBenzoatesCotyledonGlutathioneGlutathione ReductaseHelianthusImidazolesMelatoninNitric OxideNitric Oxide DonorsNitroprussideReactive Oxygen SpeciesSalinitySeedlingsStress, PhysiologicalUp-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27432590

Citation

Kaur, Harmeet, and Satish C. Bhatla. "Melatonin and Nitric Oxide Modulate Glutathione Content and Glutathione Reductase Activity in Sunflower Seedling Cotyledons Accompanying Salt Stress." Nitric Oxide : Biology and Chemistry, vol. 59, 2016, pp. 42-53.
Kaur H, Bhatla SC. Melatonin and nitric oxide modulate glutathione content and glutathione reductase activity in sunflower seedling cotyledons accompanying salt stress. Nitric Oxide. 2016;59:42-53.
Kaur, H., & Bhatla, S. C. (2016). Melatonin and nitric oxide modulate glutathione content and glutathione reductase activity in sunflower seedling cotyledons accompanying salt stress. Nitric Oxide : Biology and Chemistry, 59, 42-53. https://doi.org/10.1016/j.niox.2016.07.001
Kaur H, Bhatla SC. Melatonin and Nitric Oxide Modulate Glutathione Content and Glutathione Reductase Activity in Sunflower Seedling Cotyledons Accompanying Salt Stress. Nitric Oxide. 2016 09 30;59:42-53. PubMed PMID: 27432590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Melatonin and nitric oxide modulate glutathione content and glutathione reductase activity in sunflower seedling cotyledons accompanying salt stress. AU - Kaur,Harmeet, AU - Bhatla,Satish C, Y1 - 2016/07/16/ PY - 2016/02/10/received PY - 2016/07/13/revised PY - 2016/07/14/accepted PY - 2016/7/20/entrez PY - 2016/7/20/pubmed PY - 2017/12/22/medline KW - Glutathione KW - Glutathione reductase KW - Melatonin KW - Nitric oxide KW - Salt stress KW - Sunflower SP - 42 EP - 53 JF - Nitric oxide : biology and chemistry JO - Nitric Oxide VL - 59 N2 - The present findings demonstrate significant modulation of total glutathione content, reduced glutathione (GSH) content, oxidized glutathione (GSSG) content, GSH/GSSG ratio and glutathione reductase (GR; EC 1.6.4.2) activity in dark-grown seedling cotyledons in response to salt-stress (120 mM NaCl) in sunflower (Helianthus annuus L.) seedlings. A differential spatial distribution of GR activity (monitored by confocal laser scanning microscopic (CLSM) imaging) is also evident. Melatonin and nitric oxide (NO) differentially ameliorate salt stress effect by modulating GR activity and GSH content in seedling cotyledons. Total glutathione content (GSH + GSSG) exhibit a seedling age-dependent increase in the cotyledons, more so in salt-stressed conditions and when subjected to melatonin treatment. Seedlings raised in presence of 15 μM of melatonin exhibit significant increase in GR activity in cotyledon homogenates (10,000 g supernatant) coinciding with significant increase in GSH content. GSSG content and GSH/GSSG ratio also increased due to melatonin treatment. A correlation is thus evident in NaCl-sensitized modulation of GSH content and GR activity by melatonin. GSH content is down regulated by NO provided as 250 μM of sodium nitroprusside (SNP) although total glutathione content remained in similar range. A reversal of response (enhanced total glutathione accumulation) by NO scavenger (cPTIO) highlights the critical role of NO in modulating glutathione homeostasis. SNP lowers the activity of hydroxyindole-O-methyltransferase (HIOMT) - a regulatory enzyme in melatonin biosynthesis in control seedlings whereas its activity is upregulated in salt-stressed seedling cotyledons. Melatonin content of seedling cotyledons is also modulated by NO. NO and melatonin thus seem to modulate GR activity and GSH content during seedling growth under salt stress. SN - 1089-8611 UR - https://www.unboundmedicine.com/medline/citation/27432590/Melatonin_and_nitric_oxide_modulate_glutathione_content_and_glutathione_reductase_activity_in_sunflower_seedling_cotyledons_accompanying_salt_stress_ DB - PRIME DP - Unbound Medicine ER -