Tags

Type your tag names separated by a space and hit enter

A triple-amplification SPR electrochemiluminescence assay for chloramphenicol based on polymer enzyme-linked nanotracers and exonuclease-assisted target recycling.
Biosens Bioelectron. 2016 Dec 15; 86:477-483.BB

Abstract

The present study aimed to explore a novel triple-amplification electrochemiluminescence (ECL) assay for detecting of chloramphenicol (CAP). This strategy was based on single-stranded DNA-binding protein (SSB) and horseradish peroxidase (HRP) enzyme-linked polymer (EnVision reagent, EV) labeled on Au nanoparticles (EV-Au-SSB) as nanotracer and exonuclease-assisted target recycling. The composite probes were prepared via immunoreactions between the CdS nanocrystal (CdS NC)-functionalized partial complementary DNA and aptamer (CdSNCs/Apt-ssDNA1) as capture probes, and EV-Au-SSB as nanotracer. When the composite probe solution co-existed with CAP and Exo I, the aptamer on the capture probes preferentially combined with CAP, and then CAP-Apt and nanotracer complex were released into the solution. Subsequently, Exo I in the solution could further digest the CAP-Apt from the 3'-end of the aptamer and release CAP, which could participate in further reaction with the probes. It was worth mentioning that EV contained a large number of HRPs on its dendritic chain. In the EV-Au-SSB, Au could enhance ECL intensity of CdS NCs by surface plasmon resonance. What's more, HRPs on EV could catalyze the reaction of H2O2, which could obviously enhance ECL intensity of CdS NCs. This study demonstrated excellent performance of the triple-amplification ECL assay, which makes this aptasensor system suitable and promising for the practical application of CAP residues in fish samples. Moreover, the assay might provide a promising avenue to develop efficient aptasensors to determine small-molecule harmful substances in environmental monitoring and food safety.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Miao YB
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Ren HX
Key Laboratory of Asymmetric Synthesis and Chirotechnology of Sichuan Province, Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.
Gan N
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: ganning@nbu.edu.cn.
Zhou Y
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: zhouyou@nbu.edu.cn.
Cao Y
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
Li T
State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China. Electronic address: litianhua@nbu.edu.cn.
Chen Y
Faculty of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210000, PR China.

MeSH

Anti-Bacterial AgentsAptamers, NucleotideBiosensing TechniquesCadmium CompoundsChloramphenicolDNA-Binding ProteinsElectrochemical TechniquesEnvironmental PollutantsGoldHorseradish PeroxidaseHydrogen PeroxideLimit of DetectionLuminescent MeasurementsNanoparticlesSulfides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27434234

Citation

Miao, Yang-Bao, et al. "A Triple-amplification SPR Electrochemiluminescence Assay for Chloramphenicol Based On Polymer Enzyme-linked Nanotracers and Exonuclease-assisted Target Recycling." Biosensors & Bioelectronics, vol. 86, 2016, pp. 477-483.
Miao YB, Ren HX, Gan N, et al. A triple-amplification SPR electrochemiluminescence assay for chloramphenicol based on polymer enzyme-linked nanotracers and exonuclease-assisted target recycling. Biosens Bioelectron. 2016;86:477-483.
Miao, Y. B., Ren, H. X., Gan, N., Zhou, Y., Cao, Y., Li, T., & Chen, Y. (2016). A triple-amplification SPR electrochemiluminescence assay for chloramphenicol based on polymer enzyme-linked nanotracers and exonuclease-assisted target recycling. Biosensors & Bioelectronics, 86, 477-483. https://doi.org/10.1016/j.bios.2016.07.007
Miao YB, et al. A Triple-amplification SPR Electrochemiluminescence Assay for Chloramphenicol Based On Polymer Enzyme-linked Nanotracers and Exonuclease-assisted Target Recycling. Biosens Bioelectron. 2016 Dec 15;86:477-483. PubMed PMID: 27434234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A triple-amplification SPR electrochemiluminescence assay for chloramphenicol based on polymer enzyme-linked nanotracers and exonuclease-assisted target recycling. AU - Miao,Yang-Bao, AU - Ren,Hong-Xia, AU - Gan,Ning, AU - Zhou,You, AU - Cao,Yuting, AU - Li,Tianhua, AU - Chen,Yinji, Y1 - 2016/07/05/ PY - 2016/04/28/received PY - 2016/06/29/revised PY - 2016/07/04/accepted PY - 2016/7/20/entrez PY - 2016/7/20/pubmed PY - 2017/2/1/medline KW - Chloramphenicol KW - Horseradish peroxidase enzyme-linked polymer KW - Nanotracer KW - Triple-amplification electrochemiluminescence assay KW - single-stranded DNA-binding protein SP - 477 EP - 483 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 86 N2 - The present study aimed to explore a novel triple-amplification electrochemiluminescence (ECL) assay for detecting of chloramphenicol (CAP). This strategy was based on single-stranded DNA-binding protein (SSB) and horseradish peroxidase (HRP) enzyme-linked polymer (EnVision reagent, EV) labeled on Au nanoparticles (EV-Au-SSB) as nanotracer and exonuclease-assisted target recycling. The composite probes were prepared via immunoreactions between the CdS nanocrystal (CdS NC)-functionalized partial complementary DNA and aptamer (CdSNCs/Apt-ssDNA1) as capture probes, and EV-Au-SSB as nanotracer. When the composite probe solution co-existed with CAP and Exo I, the aptamer on the capture probes preferentially combined with CAP, and then CAP-Apt and nanotracer complex were released into the solution. Subsequently, Exo I in the solution could further digest the CAP-Apt from the 3'-end of the aptamer and release CAP, which could participate in further reaction with the probes. It was worth mentioning that EV contained a large number of HRPs on its dendritic chain. In the EV-Au-SSB, Au could enhance ECL intensity of CdS NCs by surface plasmon resonance. What's more, HRPs on EV could catalyze the reaction of H2O2, which could obviously enhance ECL intensity of CdS NCs. This study demonstrated excellent performance of the triple-amplification ECL assay, which makes this aptasensor system suitable and promising for the practical application of CAP residues in fish samples. Moreover, the assay might provide a promising avenue to develop efficient aptasensors to determine small-molecule harmful substances in environmental monitoring and food safety. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27434234/A_triple_amplification_SPR_electrochemiluminescence_assay_for_chloramphenicol_based_on_polymer_enzyme_linked_nanotracers_and_exonuclease_assisted_target_recycling_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓2]

Related Citations

More