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Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model.
Int J Antimicrob Agents. 2016 Sep; 48(3):292-7.IJ

Abstract

Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM.

Authors+Show Affiliations

Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú Km 1, Araraquara, São Paulo CEP: 14801-902, Brazil.Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú Km 1, Araraquara, São Paulo CEP: 14801-902, Brazil.Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú Km 1, Araraquara, São Paulo CEP: 14801-902, Brazil.Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú Km 1, Araraquara, São Paulo CEP: 14801-902, Brazil.Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Rodovia Araraquara-Jaú Km 1, Araraquara, São Paulo CEP: 14801-902, Brazil. Electronic address: gianninimj@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27444116

Citation

de Lacorte Singulani, Junya, et al. "Evaluation of the Efficacy of Antifungal Drugs Against Paracoccidioides Brasiliensis and Paracoccidioides Lutzii in a Galleria Mellonella Model." International Journal of Antimicrobial Agents, vol. 48, no. 3, 2016, pp. 292-7.
de Lacorte Singulani J, Scorzoni L, de Paula E Silva AC, et al. Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model. Int J Antimicrob Agents. 2016;48(3):292-7.
de Lacorte Singulani, J., Scorzoni, L., de Paula E Silva, A. C., Fusco-Almeida, A. M., & Mendes-Giannini, M. J. (2016). Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model. International Journal of Antimicrobial Agents, 48(3), 292-7. https://doi.org/10.1016/j.ijantimicag.2016.05.012
de Lacorte Singulani J, et al. Evaluation of the Efficacy of Antifungal Drugs Against Paracoccidioides Brasiliensis and Paracoccidioides Lutzii in a Galleria Mellonella Model. Int J Antimicrob Agents. 2016;48(3):292-7. PubMed PMID: 27444116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model. AU - de Lacorte Singulani,Junya, AU - Scorzoni,Liliana, AU - de Paula E Silva,Ana Carolina Alves, AU - Fusco-Almeida,Ana Marisa, AU - Mendes-Giannini,Maria José Soares, Y1 - 2016/07/05/ PY - 2016/01/21/received PY - 2016/05/17/revised PY - 2016/05/22/accepted PY - 2016/7/23/entrez PY - 2016/7/23/pubmed PY - 2017/3/7/medline KW - Alternative animal model KW - Antifungals KW - Efficacy KW - Paracoccidioidomycosis SP - 292 EP - 7 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 48 IS - 3 N2 - Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/27444116/Evaluation_of_the_efficacy_of_antifungal_drugs_against_Paracoccidioides_brasiliensis_and_Paracoccidioides_lutzii_in_a_Galleria_mellonella_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(16)30150-9 DB - PRIME DP - Unbound Medicine ER -