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Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain.
Acta Parasitol. 2016 Sep 01; 61(3):650-3.AP

Abstract

In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of V. vermiformis strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of Vermamoeba. After a week, V. vermiformis amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of Vermamoeba vermiformis in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

27447234

Citation

Reyes-Batlle, María, et al. "Isolation of Thermotolerant Vermamoeba Vermiformis Strains From Water Sources in Lanzarote Island, Canary Islands, Spain." Acta Parasitologica, vol. 61, no. 3, 2016, pp. 650-3.
Reyes-Batlle M, Wagner C, Zamora-Herrera J, et al. Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain. Acta Parasitol. 2016;61(3):650-3.
Reyes-Batlle, M., Wagner, C., Zamora-Herrera, J., Vargas-Mesa, A., Sifaoui, I., González, A. C., López-Arencibia, A., Valladares, B., Martínez-Carretero, E., Piñero, J. E., & Lorenzo-Morales, J. (2016). Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain. Acta Parasitologica, 61(3), 650-3. https://doi.org/10.1515/ap-2016-0088
Reyes-Batlle M, et al. Isolation of Thermotolerant Vermamoeba Vermiformis Strains From Water Sources in Lanzarote Island, Canary Islands, Spain. Acta Parasitol. 2016 Sep 1;61(3):650-3. PubMed PMID: 27447234.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of thermotolerant Vermamoeba vermiformis strains from water sources in Lanzarote Island, Canary Islands, Spain. AU - Reyes-Batlle,María, AU - Wagner,Carolina, AU - Zamora-Herrera,Jonadab, AU - Vargas-Mesa,Alejandro, AU - Sifaoui,Ines, AU - González,Ana C, AU - López-Arencibia,Atteneri, AU - Valladares,Basilio, AU - Martínez-Carretero,Enrique, AU - Piñero,José E, AU - Lorenzo-Morales,Jacob, PY - 2015/02/16/received PY - 2016/03/17/accepted PY - 2016/7/23/entrez PY - 2016/7/23/pubmed PY - 2017/4/27/medline SP - 650 EP - 3 JF - Acta parasitologica JO - Acta Parasitol VL - 61 IS - 3 N2 - In this study, twenty water samples were collected in the island of Lanzarote, Canary Islands, Spain in order to check for the presence of V. vermiformis strains in these samples. Water samples were cultured on 2% Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of Vermamoeba. After a week, V. vermiformis amoebae were observed in 2 of the 20 processed samples (10%) incubated at room temperature and 37°C. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing in order to confirm the identity of the isolated amoebic strains. To the best of our knowledge, this is the first report on the presence of FLA in environmental sources in Lanzarote Island and the first report of Vermamoeba vermiformis in water sources in this island. Furthermore, the two strains isolated in this study were collected in recreational areas with close contact with humans and thus awareness should be raised. SN - 1896-1851 UR - https://www.unboundmedicine.com/medline/citation/27447234/Isolation_of_thermotolerant_Vermamoeba_vermiformis_strains_from_water_sources_in_Lanzarote_Island_Canary_Islands_Spain_ L2 - https://link.springer.com/article/10.1515/ap-2016-0088 DB - PRIME DP - Unbound Medicine ER -