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l-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise.
Appl Physiol Nutr Metab. 2016 Aug; 41(8):842-849.AP

Abstract

In this study we investigated the chronic effects of oral l-glutamine and l-alanine supplementation, either in their free or dipeptide form, on glutamine-glutathione (GLN-GSH) axis and cytoprotection mediated by HSP-27 in rats submitted to resistance exercise (RE). Forty Wistar rats were distributed into 5 groups: sedentary; trained (CTRL); and trained supplemented with l-alanyl-l-glutamine, l-glutamine and l-alanine in their free form (GLN+ALA), or free l-alanine (ALA). All trained animals were submitted to a 6-week ladder-climbing protocol. Supplementations were offered in a 4% drinking water solution for 21 days prior to euthanasia. Plasma glutamine, creatine kinase (CK), myoglobin (MYO), and erythrocyte concentration of reduced GSH and glutathione disulfide (GSSG) were measured. In tibialis anterior skeletal muscle, GLN-GSH axis, thiobarbituric acid reactive substances (TBARS), and the expression of heat shock factor 1 (HSF-1), 27-kDa heat shock protein (HSP-27), and glutamine synthetase were determined. In CRTL animals, high-intensity RE reduced muscle glutamine levels and increased GSSG/GSH rate and TBARS, as well as augmented plasma CK and MYO levels. Conversely, l-glutamine-supplemented animals showed an increase in plasma and muscle levels of glutamine, with a reduction in GSSG/GSH rate, TBARS, and CK. Free l-alanine administration increased plasma glutamine concentration and lowered muscle TBARS. HSF-1 and HSP-27 were high in all supplemented groups when compared with CTRL (p < 0.05). The results presented herein demonstrate that l-glutamine supplemented with l-alanine, in both a free or dipeptide form, improve the GLN-GSH axis and promote cytoprotective effects in rats submitted to high-intensity RE training.

Authors+Show Affiliations

a Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, CEP 05508-000, Brazil.a Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, CEP 05508-000, Brazil.a Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, CEP 05508-000, Brazil.b Graduate Program of Physical Education and Health, Catholic University of Brasília, Brasília, CEP 71966-700, Brazil.c Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo (USP), São Paulo, CEP 05508-000, Brazil.a Department of Food Science and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, CEP 05508-000, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27447686

Citation

Leite, Jaqueline Santos Moreira, et al. "L-glutamine and L-alanine Supplementation Increase Glutamine-glutathione Axis and Muscle HSP-27 in Rats Trained Using a Progressive High-intensity Resistance Exercise." Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, vol. 41, no. 8, 2016, pp. 842-849.
Leite JS, Raizel R, Hypólito TM, et al. L-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise. Appl Physiol Nutr Metab. 2016;41(8):842-849.
Leite, J. S., Raizel, R., Hypólito, T. M., Rosa, T. D., Cruzat, V. F., & Tirapegui, J. (2016). L-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, 41(8), 842-849.
Leite JS, et al. L-glutamine and L-alanine Supplementation Increase Glutamine-glutathione Axis and Muscle HSP-27 in Rats Trained Using a Progressive High-intensity Resistance Exercise. Appl Physiol Nutr Metab. 2016;41(8):842-849. PubMed PMID: 27447686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - l-glutamine and l-alanine supplementation increase glutamine-glutathione axis and muscle HSP-27 in rats trained using a progressive high-intensity resistance exercise. AU - Leite,Jaqueline Santos Moreira, AU - Raizel,Raquel, AU - Hypólito,Thaís Menezes, AU - Rosa,Thiago Dos Santos, AU - Cruzat,Vinicius Fernandes, AU - Tirapegui,Julio, Y1 - 2016/03/28/ PY - 2016/7/23/pubmed PY - 2017/2/14/medline PY - 2016/7/23/entrez KW - acides aminés KW - amino acids KW - anaerobic exercise KW - cytoprotection KW - exercice anaérobie KW - glutathion KW - glutathione KW - heat shock response KW - l-alanyl-l-glutamine KW - réponse au choc thermique SP - 842 EP - 849 JF - Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme JO - Appl Physiol Nutr Metab VL - 41 IS - 8 N2 - In this study we investigated the chronic effects of oral l-glutamine and l-alanine supplementation, either in their free or dipeptide form, on glutamine-glutathione (GLN-GSH) axis and cytoprotection mediated by HSP-27 in rats submitted to resistance exercise (RE). Forty Wistar rats were distributed into 5 groups: sedentary; trained (CTRL); and trained supplemented with l-alanyl-l-glutamine, l-glutamine and l-alanine in their free form (GLN+ALA), or free l-alanine (ALA). All trained animals were submitted to a 6-week ladder-climbing protocol. Supplementations were offered in a 4% drinking water solution for 21 days prior to euthanasia. Plasma glutamine, creatine kinase (CK), myoglobin (MYO), and erythrocyte concentration of reduced GSH and glutathione disulfide (GSSG) were measured. In tibialis anterior skeletal muscle, GLN-GSH axis, thiobarbituric acid reactive substances (TBARS), and the expression of heat shock factor 1 (HSF-1), 27-kDa heat shock protein (HSP-27), and glutamine synthetase were determined. In CRTL animals, high-intensity RE reduced muscle glutamine levels and increased GSSG/GSH rate and TBARS, as well as augmented plasma CK and MYO levels. Conversely, l-glutamine-supplemented animals showed an increase in plasma and muscle levels of glutamine, with a reduction in GSSG/GSH rate, TBARS, and CK. Free l-alanine administration increased plasma glutamine concentration and lowered muscle TBARS. HSF-1 and HSP-27 were high in all supplemented groups when compared with CTRL (p < 0.05). The results presented herein demonstrate that l-glutamine supplemented with l-alanine, in both a free or dipeptide form, improve the GLN-GSH axis and promote cytoprotective effects in rats submitted to high-intensity RE training. SN - 1715-5320 UR - https://www.unboundmedicine.com/medline/citation/27447686/l_glutamine_and_l_alanine_supplementation_increase_glutamine_glutathione_axis_and_muscle_HSP_27_in_rats_trained_using_a_progressive_high_intensity_resistance_exercise_ L2 - https://cdnsciencepub.com/doi/10.1139/apnm-2016-0049?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -