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Nitric oxide alleviates silver nanoparticles (AgNps)-induced phytotoxicity in Pisum sativum seedlings.
Plant Physiol Biochem. 2017 Jan; 110:167-177.PP

Abstract

Understanding the adverse impact of nanoparticles in crop plants has emerged as one of the most interesting fields of plant research. Therefore, this study has been conducted to investigate the impact of silver nanoparticles (AgNps) on Pisium sativum seedlings. Besides this, we have also tested whether nitric oxide (NO) is capable of reducing toxicity of AgNps or not. NO has been found as one of the most fascinating molecules, capable of enhancing plant tolerance to different environmental stresses. The results of the present study showed that AgNps treatments (1000 μM and 3000 μM) significantly declined growth parameters, photosynthetic pigments and chlorophyll fluorescence of pea seedlings, which could be correlated with increased accumulation of Ag in root and shoot of pea seedlings. In contrast, addition of SNP (100 μM; a donor of NO) successfully ameliorated AgNp-induced adverse effects on these parameters as it reduced accumulation of Ag and repaired damaged tissues. Levels of oxidative stress markers (SOR, H2O2 and MDA) were enhanced while their levels significantly reduced under SNP addition. AgNps (1000 μM and 3000 μM) significantly stimulated the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) while inhibited activities of glutathione reductase (GR) and dehydroascorbate reductase (DHAR). AgNps also considerably declined the total ascorbate and glutathione contents and severely damaged leaf and root anatomical structures. On the other hand, addition of SNP further increased the level of SOD, APX, GR and DHAR and significantly increased the decreased levels of total ascorbate and glutathione contents, and repaired anatomical structures. In conclusion, this study suggests that AgNps treatments adversely decreased growth, pigments and photosynthesis due to enhanced level of Ag and oxidative stress. However, SNP addition successfully ameliorates adverse impact of AgNps on pea seedlings by regulating the Ag uptake, antioxidant system, oxidative stress and anatomical structures of root and shoot.

Authors+Show Affiliations

Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India. Electronic address: dktripathiau@gmail.com.D D Pant Interdisciplinary Research Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India.D D Pant Interdisciplinary Research Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India.Govt. Pt. R.S.T. Degree College, Bhiayathan, Surajpur, Chhattisgarh, India.Govt. Ramanuj Pratap Singhdev Post Graduate College, Baikunthpur, Koriya 497335, Chhattisgarh, India. Electronic address: vijaypratap.au@gmail.com.Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India.Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India.Nanotechnology Application Centre, University of Allahabad, Allahabad 211002, India. Electronic address: prashant2singh@gmail.com.Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi, India.Nanotechnology Application Centre, University of Allahabad, Allahabad 211002, India.D D Pant Interdisciplinary Research Laboratory, Department of Botany, University of Allahabad, Allahabad 211002, India. Electronic address: dkchauhanau@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27449300

Citation

Tripathi, Durgesh Kumar, et al. "Nitric Oxide Alleviates Silver Nanoparticles (AgNps)-induced Phytotoxicity in Pisum Sativum Seedlings." Plant Physiology and Biochemistry : PPB, vol. 110, 2017, pp. 167-177.
Tripathi DK, Singh S, Singh S, et al. Nitric oxide alleviates silver nanoparticles (AgNps)-induced phytotoxicity in Pisum sativum seedlings. Plant Physiol Biochem. 2017;110:167-177.
Tripathi, D. K., Singh, S., Singh, S., Srivastava, P. K., Singh, V. P., Singh, S., Prasad, S. M., Singh, P. K., Dubey, N. K., Pandey, A. C., & Chauhan, D. K. (2017). Nitric oxide alleviates silver nanoparticles (AgNps)-induced phytotoxicity in Pisum sativum seedlings. Plant Physiology and Biochemistry : PPB, 110, 167-177. https://doi.org/10.1016/j.plaphy.2016.06.015
Tripathi DK, et al. Nitric Oxide Alleviates Silver Nanoparticles (AgNps)-induced Phytotoxicity in Pisum Sativum Seedlings. Plant Physiol Biochem. 2017;110:167-177. PubMed PMID: 27449300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide alleviates silver nanoparticles (AgNps)-induced phytotoxicity in Pisum sativum seedlings. AU - Tripathi,Durgesh Kumar, AU - Singh,Swati, AU - Singh,Shweta, AU - Srivastava,Prabhat Kumar, AU - Singh,Vijay Pratap, AU - Singh,Samiksha, AU - Prasad,Sheo Mohan, AU - Singh,Prashant Kumar, AU - Dubey,Nawal Kishore, AU - Pandey,Avinash Chand, AU - Chauhan,Devendra Kumar, Y1 - 2016/06/15/ PY - 2016/04/05/received PY - 2016/06/12/revised PY - 2016/06/13/accepted PY - 2016/7/28/pubmed PY - 2017/4/19/medline PY - 2016/7/25/entrez KW - Anatomical structures KW - Nitric oxide KW - Oxidative stress KW - Pea seedlings KW - Silver nanoparticles SP - 167 EP - 177 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 110 N2 - Understanding the adverse impact of nanoparticles in crop plants has emerged as one of the most interesting fields of plant research. Therefore, this study has been conducted to investigate the impact of silver nanoparticles (AgNps) on Pisium sativum seedlings. Besides this, we have also tested whether nitric oxide (NO) is capable of reducing toxicity of AgNps or not. NO has been found as one of the most fascinating molecules, capable of enhancing plant tolerance to different environmental stresses. The results of the present study showed that AgNps treatments (1000 μM and 3000 μM) significantly declined growth parameters, photosynthetic pigments and chlorophyll fluorescence of pea seedlings, which could be correlated with increased accumulation of Ag in root and shoot of pea seedlings. In contrast, addition of SNP (100 μM; a donor of NO) successfully ameliorated AgNp-induced adverse effects on these parameters as it reduced accumulation of Ag and repaired damaged tissues. Levels of oxidative stress markers (SOR, H2O2 and MDA) were enhanced while their levels significantly reduced under SNP addition. AgNps (1000 μM and 3000 μM) significantly stimulated the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) while inhibited activities of glutathione reductase (GR) and dehydroascorbate reductase (DHAR). AgNps also considerably declined the total ascorbate and glutathione contents and severely damaged leaf and root anatomical structures. On the other hand, addition of SNP further increased the level of SOD, APX, GR and DHAR and significantly increased the decreased levels of total ascorbate and glutathione contents, and repaired anatomical structures. In conclusion, this study suggests that AgNps treatments adversely decreased growth, pigments and photosynthesis due to enhanced level of Ag and oxidative stress. However, SNP addition successfully ameliorates adverse impact of AgNps on pea seedlings by regulating the Ag uptake, antioxidant system, oxidative stress and anatomical structures of root and shoot. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27449300/Nitric_oxide_alleviates_silver_nanoparticles__AgNps__induced_phytotoxicity_in_Pisum_sativum_seedlings_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30236-4 DB - PRIME DP - Unbound Medicine ER -