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Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L.).
PLoS One. 2016; 11(7):e0159934.Plos

Abstract

Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15% total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar) were fed for 11-weeks with one of three experimental diets containing either fish oil (FO), wild-type Camelina oil (WCO) or transgenic Camelina oil (DCO) as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents.

Authors+Show Affiliations

Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom.Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.Biomar Ltd., North Shore Road, Grangemouth FK3 8UL, United Kingdom.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, United Kingdom.Institute of Aquaculture, School of Natural Sciences, University of Stirling, Stirling FK9 4LA, United Kingdom.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27454884

Citation

Betancor, Mónica B., et al. "Nutritional Evaluation of an EPA-DHA Oil From Transgenic Camelina Sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo Salar L.)." PloS One, vol. 11, no. 7, 2016, pp. e0159934.
Betancor MB, Sprague M, Sayanova O, et al. Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L.). PLoS ONE. 2016;11(7):e0159934.
Betancor, M. B., Sprague, M., Sayanova, O., Usher, S., Metochis, C., Campbell, P. J., Napier, J. A., & Tocher, D. R. (2016). Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L.). PloS One, 11(7), e0159934. https://doi.org/10.1371/journal.pone.0159934
Betancor MB, et al. Nutritional Evaluation of an EPA-DHA Oil From Transgenic Camelina Sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo Salar L.). PLoS ONE. 2016;11(7):e0159934. PubMed PMID: 27454884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nutritional Evaluation of an EPA-DHA Oil from Transgenic Camelina sativa in Feeds for Post-Smolt Atlantic Salmon (Salmo salar L.). AU - Betancor,Mónica B, AU - Sprague,Matthew, AU - Sayanova,Olga, AU - Usher,Sarah, AU - Metochis,Christoforos, AU - Campbell,Patrick J, AU - Napier,Johnathan A, AU - Tocher,Douglas R, Y1 - 2016/07/25/ PY - 2016/03/29/received PY - 2016/07/11/accepted PY - 2016/7/26/entrez PY - 2016/7/28/pubmed PY - 2017/7/25/medline SP - e0159934 EP - e0159934 JF - PloS one JO - PLoS ONE VL - 11 IS - 7 N2 - Vegetable oils (VO) are possible substitutes for fish oil in aquafeeds but their use is limited by their lack of omega-3 (n-3) long-chain polyunsaturated fatty acids (LC-PUFA). However, oilseed crops can be modified to produce n-3 LC-PUFA such as eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, representing a potential option to fill the gap between supply and demand of these important nutrients. Camelina sativa was metabolically engineered to produce a seed oil with around 15% total n-3 LC-PUFA to potentially substitute for fish oil in salmon feeds. Post-smolt Atlantic salmon (Salmo salar) were fed for 11-weeks with one of three experimental diets containing either fish oil (FO), wild-type Camelina oil (WCO) or transgenic Camelina oil (DCO) as added lipid source to evaluate fish performance, nutrient digestibility, tissue n-3 LC-PUFA, and metabolic impact determined by liver transcriptome analysis. The DCO diet did not affect any of the performance or health parameters studied and enhanced apparent digestibility of EPA and DHA compared to the WCO diet. The level of total n-3 LC-PUFA was higher in all the tissues of DCO-fed fish than in WCO-fed fish with levels in liver similar to those in fish fed FO. Endogenous LC-PUFA biosynthetic activity was observed in fish fed both the Camelina oil diets as indicated by the liver transcriptome and levels of intermediate metabolites such as docosapentaenoic acid, with data suggesting that the dietary combination of EPA and DHA inhibited desaturation and elongation activities. Expression of genes involved in phospholipid and triacylglycerol metabolism followed a similar pattern in fish fed DCO and WCO despite the difference in n-3 LC-PUFA contents. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27454884/Nutritional_Evaluation_of_an_EPA_DHA_Oil_from_Transgenic_Camelina_sativa_in_Feeds_for_Post_Smolt_Atlantic_Salmon__Salmo_salar_L___ L2 - http://dx.plos.org/10.1371/journal.pone.0159934 DB - PRIME DP - Unbound Medicine ER -