Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus fructicosus) Residues.
Molecules. 2016 Jul 21; 21(7)M

Abstract

Blackberry processing generates up to 20% of residues composed mainly of peel, seeds and pulp that are abundant in flavonoids. The objective of this study was to optimize the ultrasound conditions, in a closed system, for antioxidants extraction, using the response surface methodology. Blackberry (Rubus fructicosus) residues were analyzed for total phenolics, total anthocyanins, and antioxidant activity by ABTS and DPPH. The selected independent variables were ultrasound amplitude (X₁: 80%-90%) and extraction time (X₂: 10-15 min), and results were compared with conventional extraction methods. The optimal conditions for antioxidants extraction were 91% amplitude for 15 min. The results for total phenolic content and anthocyanins and antioxidant activity by ABTS and DPPH were of 1201.23 mg gallic acid equivalent (GAE)/100 g dry weight basis (dw); 379.12 mg/100 g·dw; 6318.98 µmol Trolox equivalent (TE)/100 g·dw and 9617.22 µmol TE/100 g·dw, respectively. Compared to solvent extraction methods (water and ethanol), ultrasound achieved higher extraction of all compounds except for anthocyanins. The results obtained demonstrated that ultrasound is an alternative to improve extraction yield of antioxidants from fruit residues such as blackberry.

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Authors+Show Affiliations

Zafra-Rojas QY

Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Rancho Universitario, Tulancingo de Bravo, Hidalgo 43600, Mexico. zafhry@hotmail.com.

Cruz-Cansino NS

Centro de Investigación Interdisciplinario, Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Actopan-Tilcuautla s/n. Ex hacienda La Concepción, San Agustín Tlaxiaca, Hidalgo 42160, Mexico. ncruz@uaeh.edu.mx.

Quintero-Lira A

Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Rancho Universitario, Tulancingo de Bravo, Hidalgo 43600, Mexico. auroraql@yahoo.com.mx.

Gómez-Aldapa CA

Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Centro Universitario, Carretera Pachuca-Tulancingo Km 4.5, Mineral de la Reforma, Hidalgo 42183, Mexico. cgomeza@uaeh.edu.mx.

Alanís-García E

Cervantes-Elizarrarás A

Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Rancho Universitario, Tulancingo de Bravo, Hidalgo 43600, Mexico. elizarraras.alice@yahoo.com.mx.

Güemes-Vera N

Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Rancho Universitario, Tulancingo de Bravo, Hidalgo 43600, Mexico. njgv2002@yahoo.com.mx.

Ramírez-Moreno E

MeSH

AnthocyaninsAntioxidantsChemical FractionationPhenolsPlant ExtractsReproducibility of ResultsRubusUltrasonic Waves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27455210

Citation

Zafra-Rojas, Quinatzin Y., et al. "Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus Fructicosus) Residues." Molecules (Basel, Switzerland), vol. 21, no. 7, 2016.

Zafra-Rojas QY, Cruz-Cansino NS, Quintero-Lira A, et al. Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus fructicosus) Residues. Molecules. 2016;21(7).

Zafra-Rojas, Q. Y., Cruz-Cansino, N. S., Quintero-Lira, A., Gómez-Aldapa, C. A., Alanís-García, E., Cervantes-Elizarrarás, A., Güemes-Vera, N., & Ramírez-Moreno, E. (2016). Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus fructicosus) Residues. Molecules (Basel, Switzerland), 21(7). https://doi.org/10.3390/molecules21070950

Zafra-Rojas QY, et al. Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus Fructicosus) Residues. Molecules. 2016 Jul 21;21(7) PubMed PMID: 27455210.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus fructicosus) Residues. AU - Zafra-Rojas,Quinatzin Y, AU - Cruz-Cansino,Nelly S, AU - Quintero-Lira,Aurora, AU - Gómez-Aldapa,Carlos A, AU - Alanís-García,Ernesto, AU - Cervantes-Elizarrarás,Alicia, AU - Güemes-Vera,Norma, AU - Ramírez-Moreno,Esther, Y1 - 2016/07/21/ PY - 2016/06/02/received PY - 2016/07/18/revised PY - 2016/07/19/accepted PY - 2016/7/26/entrez PY - 2016/7/28/pubmed PY - 2017/4/19/medline KW - antioxidants KW - blackberry KW - extraction KW - residues KW - ultrasound JF - Molecules (Basel, Switzerland) JO - Molecules VL - 21 IS - 7 N2 - Blackberry processing generates up to 20% of residues composed mainly of peel, seeds and pulp that are abundant in flavonoids. The objective of this study was to optimize the ultrasound conditions, in a closed system, for antioxidants extraction, using the response surface methodology. Blackberry (Rubus fructicosus) residues were analyzed for total phenolics, total anthocyanins, and antioxidant activity by ABTS and DPPH. The selected independent variables were ultrasound amplitude (X₁: 80%-90%) and extraction time (X₂: 10-15 min), and results were compared with conventional extraction methods. The optimal conditions for antioxidants extraction were 91% amplitude for 15 min. The results for total phenolic content and anthocyanins and antioxidant activity by ABTS and DPPH were of 1201.23 mg gallic acid equivalent (GAE)/100 g dry weight basis (dw); 379.12 mg/100 g·dw; 6318.98 µmol Trolox equivalent (TE)/100 g·dw and 9617.22 µmol TE/100 g·dw, respectively. Compared to solvent extraction methods (water and ethanol), ultrasound achieved higher extraction of all compounds except for anthocyanins. The results obtained demonstrated that ultrasound is an alternative to improve extraction yield of antioxidants from fruit residues such as blackberry. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/27455210/Application_of_Ultrasound_in_a_Closed_System:_Optimum_Condition_for_Antioxidants_Extraction_of_Blackberry__Rubus_fructicosus__Residues_ DB - PRIME DP - Unbound Medicine ER -

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Application of Ultrasound in a Closed System: Optimum Condition for Antioxidants Extraction of Blackberry (Rubus fructicosus) Residues.Molecules. 2016 Jul 21; 21(7)M