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Transferring Desirable Genes from Agropyron cristatum 7P Chromosome into Common Wheat.
PLoS One. 2016; 11(7):e0159577.Plos

Abstract

Wheat-Agropyron cristatum 7P disomic addition line Ⅱ-5-1, derived from the distant hybridization between A. cristatum (2n = 4x = 28, PPPP) and the common wheat cv. Fukuhokomugi (Fukuho), displays numerous desirable agronomic traits, including enhanced thousand-grain weight, smaller flag leaf, and enhanced tolerance to drought. In order to transfer these traits into common wheat, Ⅱ-5-1 was induced by 60Co-γ ray, leading to the creation of 18 translocation lines and three deletion lines. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) indicated that multiple wheat chromosomes were involved in the translocation events, including chromosome 2A, 3A, 5A, 7A, 3B, 5B, 7B, 3D and 7D. A. cristatum 7P chromosome was divided into 15 chromosomal bins with fifty-five sequence-tagged site (STS) markers specific to A. cristatum 7P chromosome. Seven and eight chromosomal bins were located on 7PS and 7PL, respectively. The above-mentioned translocation and deletion lines each contained different, yet overlapping 7P chromosomal fragments, covering the entire A. cristatum 7P chromosome. Three translocation lines (7PT-13, 7PT-14 and 7PT-17) and three deletion lines (del-1, del-2 and del-3), which contained the common chromosomal bins 7PS1-3, displayed higher thousand-grain weigh than Fukuho, suggesting that potential genes conferring high thousand-grain weigh might be located on these chromosomal bins. Therefore, wheat-A. cristatum 7P translocation lines with elite traits will be useful as novel germplasms for wheat genetic improvement.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27459347

Citation

Lu, Mingjie, et al. "Transferring Desirable Genes From Agropyron Cristatum 7P Chromosome Into Common Wheat." PloS One, vol. 11, no. 7, 2016, pp. e0159577.
Lu M, Lu Y, Li H, et al. Transferring Desirable Genes from Agropyron cristatum 7P Chromosome into Common Wheat. PLoS One. 2016;11(7):e0159577.
Lu, M., Lu, Y., Li, H., Pan, C., Guo, Y., Zhang, J., Yang, X., Li, X., Liu, W., & Li, L. (2016). Transferring Desirable Genes from Agropyron cristatum 7P Chromosome into Common Wheat. PloS One, 11(7), e0159577. https://doi.org/10.1371/journal.pone.0159577
Lu M, et al. Transferring Desirable Genes From Agropyron Cristatum 7P Chromosome Into Common Wheat. PLoS One. 2016;11(7):e0159577. PubMed PMID: 27459347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transferring Desirable Genes from Agropyron cristatum 7P Chromosome into Common Wheat. AU - Lu,Mingjie, AU - Lu,Yuqing, AU - Li,Huanhuan, AU - Pan,Cuili, AU - Guo,Yong, AU - Zhang,Jinpeng, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Liu,Weihua, AU - Li,Lihui, Y1 - 2016/07/26/ PY - 2016/04/14/received PY - 2016/07/04/accepted PY - 2016/7/27/entrez PY - 2016/7/28/pubmed PY - 2017/7/25/medline SP - e0159577 EP - e0159577 JF - PloS one JO - PLoS One VL - 11 IS - 7 N2 - Wheat-Agropyron cristatum 7P disomic addition line Ⅱ-5-1, derived from the distant hybridization between A. cristatum (2n = 4x = 28, PPPP) and the common wheat cv. Fukuhokomugi (Fukuho), displays numerous desirable agronomic traits, including enhanced thousand-grain weight, smaller flag leaf, and enhanced tolerance to drought. In order to transfer these traits into common wheat, Ⅱ-5-1 was induced by 60Co-γ ray, leading to the creation of 18 translocation lines and three deletion lines. Genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH) indicated that multiple wheat chromosomes were involved in the translocation events, including chromosome 2A, 3A, 5A, 7A, 3B, 5B, 7B, 3D and 7D. A. cristatum 7P chromosome was divided into 15 chromosomal bins with fifty-five sequence-tagged site (STS) markers specific to A. cristatum 7P chromosome. Seven and eight chromosomal bins were located on 7PS and 7PL, respectively. The above-mentioned translocation and deletion lines each contained different, yet overlapping 7P chromosomal fragments, covering the entire A. cristatum 7P chromosome. Three translocation lines (7PT-13, 7PT-14 and 7PT-17) and three deletion lines (del-1, del-2 and del-3), which contained the common chromosomal bins 7PS1-3, displayed higher thousand-grain weigh than Fukuho, suggesting that potential genes conferring high thousand-grain weigh might be located on these chromosomal bins. Therefore, wheat-A. cristatum 7P translocation lines with elite traits will be useful as novel germplasms for wheat genetic improvement. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27459347/Transferring_Desirable_Genes_from_Agropyron_cristatum_7P_Chromosome_into_Common_Wheat_ L2 - https://dx.plos.org/10.1371/journal.pone.0159577 DB - PRIME DP - Unbound Medicine ER -