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Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay.
Sci Rep. 2016 07 27; 6:30577.SR

Abstract

A novel ternary composite of hemin-graphene-Au nanorods (H-RGO-Au NRs) with high electrocatalytic activity was synthesized by a simple method. And this ternary composite was firstly used in construction of electrochemiluminescence (ECL) immunosensor due to its double-quenching effect of quantum dots (QDs). Based on the high electrocatalytic activity of ternary complexes for the reduction of H2O2 which acted as the coreactant of QDs-based ECL, as a result, the ECL intensity of QDs decreased. Besides, due to the ECL resonance energy transfer (ECL-RET) strategy between the large amount of Au nanorods (Au NRs) on the ternary composite surface and the CdS:Eu QDs, the ECL intensity of QDs was further quenched. Based on the double-quenching effect, a novel ultrasensitive ECL immunoassay method for detection of carcinoembryonic antigen (CEA) which is used as a model biomarker analyte was proposed. The designed immunoassay method showed a linear range from 0.01 pg mL(-1) to 1.0 ng mL(-1) with a detection limit of 0.01 pg mL(-1). The method showing low detection limit, good stability and acceptable fabrication reproducibility, provided a new approach for ECL immunoassay sensing and significant prospect for practical application.

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Authors+Show Affiliations

Liu J
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
Cui M
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China. Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Shandong Normal University, Jinan 250014, P. R. China.
Zhou H
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.
Zhang S
Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, P. R. China.

MeSH

Biosensing TechniquesCadmium CompoundsCarcinoembryonic AntigenElectrochemical TechniquesEuropiumGraphiteHeminHumansImmunoassayLimit of DetectionLuminescent MeasurementsMicroscopy, Electron, TransmissionNanotubesQuantum DotsReproducibility of ResultsSelenium Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27460868

Citation

Liu, Jing, et al. "Efficient Double-quenching of Electrochemiluminescence From CdS:Eu QDs By hemin-graphene-Au Nanorods Ternary Composite for Ultrasensitive Immunoassay." Scientific Reports, vol. 6, 2016, p. 30577.
Liu J, Cui M, Zhou H, et al. Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay. Sci Rep. 2016;6:30577.
Liu, J., Cui, M., Zhou, H., & Zhang, S. (2016). Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay. Scientific Reports, 6, 30577. https://doi.org/10.1038/srep30577
Liu J, et al. Efficient Double-quenching of Electrochemiluminescence From CdS:Eu QDs By hemin-graphene-Au Nanorods Ternary Composite for Ultrasensitive Immunoassay. Sci Rep. 2016 07 27;6:30577. PubMed PMID: 27460868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient double-quenching of electrochemiluminescence from CdS:Eu QDs by hemin-graphene-Au nanorods ternary composite for ultrasensitive immunoassay. AU - Liu,Jing, AU - Cui,Meirong, AU - Zhou,Hong, AU - Zhang,Shusheng, Y1 - 2016/07/27/ PY - 2016/04/19/received PY - 2016/07/04/accepted PY - 2016/7/28/entrez PY - 2016/7/28/pubmed PY - 2018/4/18/medline SP - 30577 EP - 30577 JF - Scientific reports JO - Sci Rep VL - 6 N2 - A novel ternary composite of hemin-graphene-Au nanorods (H-RGO-Au NRs) with high electrocatalytic activity was synthesized by a simple method. And this ternary composite was firstly used in construction of electrochemiluminescence (ECL) immunosensor due to its double-quenching effect of quantum dots (QDs). Based on the high electrocatalytic activity of ternary complexes for the reduction of H2O2 which acted as the coreactant of QDs-based ECL, as a result, the ECL intensity of QDs decreased. Besides, due to the ECL resonance energy transfer (ECL-RET) strategy between the large amount of Au nanorods (Au NRs) on the ternary composite surface and the CdS:Eu QDs, the ECL intensity of QDs was further quenched. Based on the double-quenching effect, a novel ultrasensitive ECL immunoassay method for detection of carcinoembryonic antigen (CEA) which is used as a model biomarker analyte was proposed. The designed immunoassay method showed a linear range from 0.01 pg mL(-1) to 1.0 ng mL(-1) with a detection limit of 0.01 pg mL(-1). The method showing low detection limit, good stability and acceptable fabrication reproducibility, provided a new approach for ECL immunoassay sensing and significant prospect for practical application. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27460868/Efficient_double_quenching_of_electrochemiluminescence_from_CdS:Eu_QDs_by_hemin_graphene_Au_nanorods_ternary_composite_for_ultrasensitive_immunoassay_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓5 ↑35]

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