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Genotoxicity assessment and oxidative stress responses in freshwater African catfish Clarias gariepinus exposed to fenthion formulations.
Drug Chem Toxicol. 2017 Jul; 40(3):273-280.DC

Abstract

Fenthion is one of the most widely used organophosphate insecticides for the control of many varieties of pests in Nigeria. The genotoxic effect of the pesticide was evaluated in the blood erythrocytes of Clarias gariepinus using the micronucleus (MN) test. The oxidative stress parameters were also studied in the liver and gill tissues. Fish were exposed to 2.0, 4.0, and 8.0 mgL-1 of fenthion and sampling was done on days 1, 7, 14, 21 and after 7-day recovery. Micronuclei induction was highest (7.55) on day 14 at all concentrations in the peripheral blood cells. Oxidative stress was evidenced by increased lipid peroxidation (LPO). Maximum LPO values of 62.47% and 71.17% were observed in the gill and liver tissues respectively in C. gariepinus exposed to 8.0 mgL-1 concentration of fenthion. There were alterations in the values of reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) during the exposure and recovery periods. The 7-day recovery period was not adequate to eliminate fenthion-induced changes as LPO, CAT, and GR activity remain elevated. However, MN frequency and activity of SOD, GSH, and GPx (except at 8.0 mgL-1) recovered. The present findings give further credence on the integrated use of MN test and oxidative stress parameters in risk assessment of pollutants in aquatic ecosystem.

Authors+Show Affiliations

a Molecular Biology and Biotechnology Division, Department of Zoology and Environmental Biology, University of Nigeria Nsukka , Nigeria.b Department of Biological Science , Federal University Lokoja , Nigeria.c Department of Biology , Microbiology and Biotechnology, Federal University Ndufu-Alike , Ikwo , Nigeria.d Department of Crop Science and Land Scape Management , Ebonyi State University Abakaliki , Nigeria.e Department of Vocational Teacher Education , University of Nigeria Nsukka , Nigeria , and.f Department of Plant Science and Biotechnology , University of Nigeria Nsukka , Nigeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27460958

Citation

Nwani, Christopher Didigwu, et al. "Genotoxicity Assessment and Oxidative Stress Responses in Freshwater African Catfish Clarias Gariepinus Exposed to Fenthion Formulations." Drug and Chemical Toxicology, vol. 40, no. 3, 2017, pp. 273-280.
Nwani CD, Somdare PO, Ogueji EO, et al. Genotoxicity assessment and oxidative stress responses in freshwater African catfish Clarias gariepinus exposed to fenthion formulations. Drug Chem Toxicol. 2017;40(3):273-280.
Nwani, C. D., Somdare, P. O., Ogueji, E. O., Nwani, J. C., Ukonze, J. A., & Nwadinigwe, A. O. (2017). Genotoxicity assessment and oxidative stress responses in freshwater African catfish Clarias gariepinus exposed to fenthion formulations. Drug and Chemical Toxicology, 40(3), 273-280. https://doi.org/10.1080/01480545.2016.1209772
Nwani CD, et al. Genotoxicity Assessment and Oxidative Stress Responses in Freshwater African Catfish Clarias Gariepinus Exposed to Fenthion Formulations. Drug Chem Toxicol. 2017;40(3):273-280. PubMed PMID: 27460958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity assessment and oxidative stress responses in freshwater African catfish Clarias gariepinus exposed to fenthion formulations. AU - Nwani,Christopher Didigwu, AU - Somdare,Peace Onas, AU - Ogueji,Emmanuel Okechukwu, AU - Nwani,Jacinta Chinedu, AU - Ukonze,Juliana Adimonye, AU - Nwadinigwe,Alfreda O, Y1 - 2016/07/26/ PY - 2016/7/28/pubmed PY - 2017/9/7/medline PY - 2016/7/28/entrez KW - Clarias gariepinus KW - Fenthion KW - lipid peroxidation KW - micronuclei KW - oxidative stress SP - 273 EP - 280 JF - Drug and chemical toxicology JO - Drug Chem Toxicol VL - 40 IS - 3 N2 - Fenthion is one of the most widely used organophosphate insecticides for the control of many varieties of pests in Nigeria. The genotoxic effect of the pesticide was evaluated in the blood erythrocytes of Clarias gariepinus using the micronucleus (MN) test. The oxidative stress parameters were also studied in the liver and gill tissues. Fish were exposed to 2.0, 4.0, and 8.0 mgL-1 of fenthion and sampling was done on days 1, 7, 14, 21 and after 7-day recovery. Micronuclei induction was highest (7.55) on day 14 at all concentrations in the peripheral blood cells. Oxidative stress was evidenced by increased lipid peroxidation (LPO). Maximum LPO values of 62.47% and 71.17% were observed in the gill and liver tissues respectively in C. gariepinus exposed to 8.0 mgL-1 concentration of fenthion. There were alterations in the values of reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) during the exposure and recovery periods. The 7-day recovery period was not adequate to eliminate fenthion-induced changes as LPO, CAT, and GR activity remain elevated. However, MN frequency and activity of SOD, GSH, and GPx (except at 8.0 mgL-1) recovered. The present findings give further credence on the integrated use of MN test and oxidative stress parameters in risk assessment of pollutants in aquatic ecosystem. SN - 1525-6014 UR - https://www.unboundmedicine.com/medline/citation/27460958/Genotoxicity_assessment_and_oxidative_stress_responses_in_freshwater_African_catfish_Clarias_gariepinus_exposed_to_fenthion_formulations_ L2 - http://www.tandfonline.com/doi/full/10.1080/01480545.2016.1209772 DB - PRIME DP - Unbound Medicine ER -