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Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves.

Abstract

BACKGROUND

Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities.

METHODS

The leaves were collected, powdered and extracted with methanol. The extract was then concentrated and successively fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were investigated for free radical scavenging, thrombolytic and membrane stabilizing activities.

RESULTS

In case of 1,1 diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging assays, the crude methanol extract of the leaves showed the highest free radical scavenging activity among the tested materials including standard ascorbic acid (p = 0.0000). Besides, this extract was also found significantly rich (p = 0.0000) in phenolics and flavonoids compared to other organic fractions. In thrombolytic study, the petroleum ether fraction exhibited significantly stronger thrombolysis (p = 0.024) than other leaf extractives but was weaker than the standard streptokinase. In membrane stabilizing assay, the activity of chloroform fraction was similar to that of standard acetylsalicylic acid (p = 1.000) in hypotonic solution induced hemolysis. However, membrane stabilization activity of this chloroform fraction was found significantly stronger than that of the standard (p = 0.0000) in heat induced hemolysis.

CONCLUSION

This study has revealed the medicinal capabilities of different organic fractions of C. citrinus displaying free radical scavenging, thrombolysis and membrane stabilizing antiinflammatory potentials. Further bioactivity guided isolation is required to obtain pharmacologically secondary metabolites.

Authors+Show Affiliations

Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, 1000, Bangladesh.Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, 1000, Bangladesh. msr@du.ac.bd.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27460997

Citation

Ahmed, Farhana, and Mohammad Sharifur Rahman. "Preliminary Assessment of Free Radical Scavenging, Thrombolytic and Membrane Stabilizing Capabilities of Organic Fractions of Callistemon Citrinus (Curtis.) Skeels Leaves." BMC Complementary and Alternative Medicine, vol. 16, 2016, p. 247.
Ahmed F, Rahman MS. Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves. BMC Complement Altern Med. 2016;16:247.
Ahmed, F., & Rahman, M. S. (2016). Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves. BMC Complementary and Alternative Medicine, 16, p. 247. doi:10.1186/s12906-016-1239-1.
Ahmed F, Rahman MS. Preliminary Assessment of Free Radical Scavenging, Thrombolytic and Membrane Stabilizing Capabilities of Organic Fractions of Callistemon Citrinus (Curtis.) Skeels Leaves. BMC Complement Altern Med. 2016 Jul 26;16:247. PubMed PMID: 27460997.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves. AU - Ahmed,Farhana, AU - Rahman,Mohammad Sharifur, Y1 - 2016/07/26/ PY - 2015/11/28/received PY - 2016/07/23/accepted PY - 2016/7/28/entrez PY - 2016/7/28/pubmed PY - 2017/2/10/medline KW - Antiinflammatory KW - Antioxidant KW - Callistemon citrinus KW - Free radical scavenging KW - Herbal KW - Membrane stabilization KW - Myrtaceae KW - Thrombolytic SP - 247 EP - 247 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 N2 - BACKGROUND: Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities. METHODS: The leaves were collected, powdered and extracted with methanol. The extract was then concentrated and successively fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were investigated for free radical scavenging, thrombolytic and membrane stabilizing activities. RESULTS: In case of 1,1 diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging assays, the crude methanol extract of the leaves showed the highest free radical scavenging activity among the tested materials including standard ascorbic acid (p = 0.0000). Besides, this extract was also found significantly rich (p = 0.0000) in phenolics and flavonoids compared to other organic fractions. In thrombolytic study, the petroleum ether fraction exhibited significantly stronger thrombolysis (p = 0.024) than other leaf extractives but was weaker than the standard streptokinase. In membrane stabilizing assay, the activity of chloroform fraction was similar to that of standard acetylsalicylic acid (p = 1.000) in hypotonic solution induced hemolysis. However, membrane stabilization activity of this chloroform fraction was found significantly stronger than that of the standard (p = 0.0000) in heat induced hemolysis. CONCLUSION: This study has revealed the medicinal capabilities of different organic fractions of C. citrinus displaying free radical scavenging, thrombolysis and membrane stabilizing antiinflammatory potentials. Further bioactivity guided isolation is required to obtain pharmacologically secondary metabolites. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27460997/Preliminary_assessment_of_free_radical_scavenging_thrombolytic_and_membrane_stabilizing_capabilities_of_organic_fractions_of_Callistemon_citrinus__Curtis___skeels_leaves_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1239-1 DB - PRIME DP - Unbound Medicine ER -