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Discovery of midgut genes for the RNA interference control of corn rootworm.
Sci Rep. 2016 07 28; 6:30542.SR

Abstract

RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based insect feeding assays using double-stranded RNA (dsRNA) targeting dvssj1 and dvssj2 demonstrate targeted mRNA suppression, larval growth inhibition, and mortality. In RNAi treated WCR, injury to the midgut was manifested by "blebbing" of the midgut epithelium into the gut lumen. Ultrastructural examination of midgut epithelial cells revealed apoptosis and regenerative activities. Transgenic plants expressing dsRNA targeting dvssj1 show insecticidal activity and significant plant protection from WCR damage. The data indicate that dvssj1 and dvssj2 are effective gene targets for the control of WCR using RNAi technology, by apparent suppression of production of their respective smooth septate junction membrane proteins located within the intestinal lining, leading to growth inhibition and mortality.

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Authors+Show Affiliations

Hu X
DuPont Pioneer, Johnston, IA, USA.
Richtman NM
DuPont Pioneer, Johnston, IA, USA.
Zhao JZ
DuPont Pioneer, Johnston, IA, USA.
Duncan KE
DuPont Pioneer, Wilmington, DE, USA.
Niu X
DuPont Pioneer, Johnston, IA, USA.
Procyk LA
DuPont Pioneer, Johnston, IA, USA.
Oneal MA
DuPont Pioneer, Johnston, IA, USA.
Kernodle BM
DuPont Pioneer, Johnston, IA, USA.
Steimel JP
DuPont Pioneer, Johnston, IA, USA.
Crane VC
DuPont Pioneer, Johnston, IA, USA.
Sandahl G
DuPont Pioneer, Johnston, IA, USA.
Ritland JL
DuPont Pioneer, Johnston, IA, USA.
Howard RJ
DuPont Pioneer, Wilmington, DE, USA.
Presnail JK
DuPont Pioneer, Johnston, IA, USA.
Lu AL
DuPont Pioneer, Johnston, IA, USA.
Wu G
DuPont Pioneer, Johnston, IA, USA.

MeSH

AnimalsColeopteraGastrointestinal TractGene Expression RegulationInsect ProteinsLarvaPest Control, BiologicalPlant RootsPlants, Genetically ModifiedRNA InterferenceRNA, Double-StrandedZea mays

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27464714

Citation

Hu, Xu, et al. "Discovery of Midgut Genes for the RNA Interference Control of Corn Rootworm." Scientific Reports, vol. 6, 2016, p. 30542.
Hu X, Richtman NM, Zhao JZ, et al. Discovery of midgut genes for the RNA interference control of corn rootworm. Sci Rep. 2016;6:30542.
Hu, X., Richtman, N. M., Zhao, J. Z., Duncan, K. E., Niu, X., Procyk, L. A., Oneal, M. A., Kernodle, B. M., Steimel, J. P., Crane, V. C., Sandahl, G., Ritland, J. L., Howard, R. J., Presnail, J. K., Lu, A. L., & Wu, G. (2016). Discovery of midgut genes for the RNA interference control of corn rootworm. Scientific Reports, 6, 30542. https://doi.org/10.1038/srep30542
Hu X, et al. Discovery of Midgut Genes for the RNA Interference Control of Corn Rootworm. Sci Rep. 2016 07 28;6:30542. PubMed PMID: 27464714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discovery of midgut genes for the RNA interference control of corn rootworm. AU - Hu,Xu, AU - Richtman,Nina M, AU - Zhao,Jian-Zhou, AU - Duncan,Keith E, AU - Niu,Xiping, AU - Procyk,Lisa A, AU - Oneal,Meghan A, AU - Kernodle,Bliss M, AU - Steimel,Joseph P, AU - Crane,Virginia C, AU - Sandahl,Gary, AU - Ritland,Julie L, AU - Howard,Richard J, AU - Presnail,James K, AU - Lu,Albert L, AU - Wu,Gusui, Y1 - 2016/07/28/ PY - 2016/04/07/received PY - 2016/07/06/accepted PY - 2016/7/29/entrez PY - 2016/7/29/pubmed PY - 2018/4/18/medline SP - 30542 EP - 30542 JF - Scientific reports JO - Sci Rep VL - 6 N2 - RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based insect feeding assays using double-stranded RNA (dsRNA) targeting dvssj1 and dvssj2 demonstrate targeted mRNA suppression, larval growth inhibition, and mortality. In RNAi treated WCR, injury to the midgut was manifested by "blebbing" of the midgut epithelium into the gut lumen. Ultrastructural examination of midgut epithelial cells revealed apoptosis and regenerative activities. Transgenic plants expressing dsRNA targeting dvssj1 show insecticidal activity and significant plant protection from WCR damage. The data indicate that dvssj1 and dvssj2 are effective gene targets for the control of WCR using RNAi technology, by apparent suppression of production of their respective smooth septate junction membrane proteins located within the intestinal lining, leading to growth inhibition and mortality. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27464714/Discovery_of_midgut_genes_for_the_RNA_interference_control_of_corn_rootworm_ DB - PRIME DP - Unbound Medicine ER -