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Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes.
. 2016 10 01; 311(4):R649-R657.

Abstract

The family Channichthyidae or "icefishes" (suborder Notothenioidei) represents the only vertebrates lacking hemoglobin (Hb) as adults. Several icefish species also do not express cardiac myoglobin (Mb). We address how levels of proteins involved in iron (Fe) processing (transport, sequestration, and export) vary among white- and red-blooded notothenioids, and whether absence of Hb and/or Mb in channichthyids is accompanied by expansion of contents of Fe-binding proteins to protect against unchaperoned Fe. Levels of transferrin (Tf), ferritin (Ft), ceruloplasmin (Cp), and non-heme Fe were quantified in plasma, serum, and/or nonhematopoietic tissues (cardiac ventricle, skeletal muscle, and liver) from species of white-blooded (Chaenocephalus aceratus, Champsocephalus gunnari, Chionodraco rastrospinosus, Pseudochaenichthys georgianus) (the first two species not expressing Mb) and red-blooded (Notothenia coriiceps, Gobionotothen gibberifrons) notothenioids. We also measured levels of ascorbate (Asc), a mediator of Fe uptake. While plasma concentrations of Tf and tissue levels of Asc are similar among species, concentrations of plasma Asc are lower in white-blooded species. Concentrations of Ft and non-heme Fe and activities of Cp are also generally reduced in icefishes compared with red-blooded notothenioids. The presence of cardiac Mb in some icefish species does not appear to influence levels of proteins involved in Fe processing. To address further the question of Fe sequestration within a physiological context, we account for well-characterized differences in blood volume and heart mass among white- and red-blooded notothenioids. We report that total contents of plasma Tf are greater, while ventricle non-heme Fe is at least at parity in white- vs. red-blooded species.

Authors+Show Affiliations

Department of Biological Sciences, Ohio University, Athens, Ohio; and.Institute of Arctic Biology, University of Alaska, Fairbanks, Alaska.Department of Biological Sciences, Ohio University, Athens, Ohio; and crockett@ohio.edu.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

27465736

Citation

Kuhn, Donald E., et al. "Expansion of Capacities for Iron Transport and Sequestration Reflects Plasma Volumes and Heart Mass Among White-blooded Notothenioid Fishes." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 311, no. 4, 2016, pp. R649-R657.
Kuhn DE, O'Brien KM, Crockett EL. Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes. Am J Physiol Regul Integr Comp Physiol. 2016;311(4):R649-R657.
Kuhn, D. E., O'Brien, K. M., & Crockett, E. L. (2016). Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 311(4), R649-R657. https://doi.org/10.1152/ajpregu.00188.2016
Kuhn DE, O'Brien KM, Crockett EL. Expansion of Capacities for Iron Transport and Sequestration Reflects Plasma Volumes and Heart Mass Among White-blooded Notothenioid Fishes. Am J Physiol Regul Integr Comp Physiol. 2016 10 1;311(4):R649-R657. PubMed PMID: 27465736.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of capacities for iron transport and sequestration reflects plasma volumes and heart mass among white-blooded notothenioid fishes. AU - Kuhn,Donald E, AU - O'Brien,Kristin M, AU - Crockett,Elizabeth L, Y1 - 2016/07/27/ PY - 2016/05/06/received PY - 2016/07/25/accepted PY - 2016/7/29/pubmed PY - 2017/6/27/medline PY - 2016/7/29/entrez KW - Antarctic notothenioid fishes KW - ceruloplasmin KW - ferritin KW - iron processing KW - transferrin SP - R649 EP - R657 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am. J. Physiol. Regul. Integr. Comp. Physiol. VL - 311 IS - 4 N2 - The family Channichthyidae or "icefishes" (suborder Notothenioidei) represents the only vertebrates lacking hemoglobin (Hb) as adults. Several icefish species also do not express cardiac myoglobin (Mb). We address how levels of proteins involved in iron (Fe) processing (transport, sequestration, and export) vary among white- and red-blooded notothenioids, and whether absence of Hb and/or Mb in channichthyids is accompanied by expansion of contents of Fe-binding proteins to protect against unchaperoned Fe. Levels of transferrin (Tf), ferritin (Ft), ceruloplasmin (Cp), and non-heme Fe were quantified in plasma, serum, and/or nonhematopoietic tissues (cardiac ventricle, skeletal muscle, and liver) from species of white-blooded (Chaenocephalus aceratus, Champsocephalus gunnari, Chionodraco rastrospinosus, Pseudochaenichthys georgianus) (the first two species not expressing Mb) and red-blooded (Notothenia coriiceps, Gobionotothen gibberifrons) notothenioids. We also measured levels of ascorbate (Asc), a mediator of Fe uptake. While plasma concentrations of Tf and tissue levels of Asc are similar among species, concentrations of plasma Asc are lower in white-blooded species. Concentrations of Ft and non-heme Fe and activities of Cp are also generally reduced in icefishes compared with red-blooded notothenioids. The presence of cardiac Mb in some icefish species does not appear to influence levels of proteins involved in Fe processing. To address further the question of Fe sequestration within a physiological context, we account for well-characterized differences in blood volume and heart mass among white- and red-blooded notothenioids. We report that total contents of plasma Tf are greater, while ventricle non-heme Fe is at least at parity in white- vs. red-blooded species. SN - 1522-1490 UR - https://www.unboundmedicine.com/medline/citation/27465736/Expansion_of_capacities_for_iron_transport_and_sequestration_reflects_plasma_volumes_and_heart_mass_among_white_blooded_notothenioid_fishes_ L2 - https://journals.physiology.org/doi/10.1152/ajpregu.00188.2016?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -