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Broad Screening and Identification of Novel Psychoactive Substances in Plasma by High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching.
J Anal Toxicol. 2016 Sep; 40(7):519-28.JA

Abstract

Drug abuse is today a growing global problem. Often the consumers are not aware about the type of substances they are using and the correlated risks. In recent years, new psychoactive substances (NPS) appeared in the illicit market. The presence of NPS, such as synthetic cathinones, cannabinoids and phenethylamines, which are known to be pharmacologically and toxicologically hazardous, has been frequently reported. The aim of this study was the development of a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for a broad screening of NPS in plasma. Data acquisition was in MS/MS and full-scan modes and the method was validated for 25 NPS belonging to different chemical classes. Quantitative results have been obtained for these analytes with limits of quantification ranging from 0.03 to 0.4 ng/mL. The method was proven to be suitable for the screening of additional substances; to this aim, a post-run library matching was conducted for every sample with an in-house database containing over 300 NPS and known metabolites. The library may be constantly expanded with new drugs, in order to obtain a broad screening of NPS in biological matrices.

Authors+Show Affiliations

Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy camilla.montesano@uniroma1.it.Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.Department of Scientific Investigation (RIS), Carabinieri, Viale di Tor di Quinto 119, 00191 Rome, Italy.Department of Scientific Investigation (RIS), Carabinieri, Viale di Tor di Quinto 119, 00191 Rome, Italy.Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via C. Lerici 1, 64023 Mosciano Sant'Angelo, TE, Italy.Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via C. Lerici 1, 64023 Mosciano Sant'Angelo, TE, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27466313

Citation

Montesano, Camilla, et al. "Broad Screening and Identification of Novel Psychoactive Substances in Plasma By High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching." Journal of Analytical Toxicology, vol. 40, no. 7, 2016, pp. 519-28.
Montesano C, Vannutelli G, Gregori A, et al. Broad Screening and Identification of Novel Psychoactive Substances in Plasma by High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching. J Anal Toxicol. 2016;40(7):519-28.
Montesano, C., Vannutelli, G., Gregori, A., Ripani, L., Compagnone, D., Curini, R., & Sergi, M. (2016). Broad Screening and Identification of Novel Psychoactive Substances in Plasma by High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching. Journal of Analytical Toxicology, 40(7), 519-28. https://doi.org/10.1093/jat/bkw043
Montesano C, et al. Broad Screening and Identification of Novel Psychoactive Substances in Plasma By High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching. J Anal Toxicol. 2016;40(7):519-28. PubMed PMID: 27466313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Broad Screening and Identification of Novel Psychoactive Substances in Plasma by High-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Post-run Library Matching. AU - Montesano,Camilla, AU - Vannutelli,Gabriele, AU - Gregori,Adolfo, AU - Ripani,Luigi, AU - Compagnone,Dario, AU - Curini,Roberta, AU - Sergi,Manuel, Y1 - 2016/07/27/ PY - 2016/04/25/received PY - 2016/03/26/accepted PY - 2016/7/29/entrez PY - 2016/7/29/pubmed PY - 2018/1/13/medline SP - 519 EP - 28 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 40 IS - 7 N2 - Drug abuse is today a growing global problem. Often the consumers are not aware about the type of substances they are using and the correlated risks. In recent years, new psychoactive substances (NPS) appeared in the illicit market. The presence of NPS, such as synthetic cathinones, cannabinoids and phenethylamines, which are known to be pharmacologically and toxicologically hazardous, has been frequently reported. The aim of this study was the development of a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) method for a broad screening of NPS in plasma. Data acquisition was in MS/MS and full-scan modes and the method was validated for 25 NPS belonging to different chemical classes. Quantitative results have been obtained for these analytes with limits of quantification ranging from 0.03 to 0.4 ng/mL. The method was proven to be suitable for the screening of additional substances; to this aim, a post-run library matching was conducted for every sample with an in-house database containing over 300 NPS and known metabolites. The library may be constantly expanded with new drugs, in order to obtain a broad screening of NPS in biological matrices. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/27466313/Broad_Screening_and_Identification_of_Novel_Psychoactive_Substances_in_Plasma_by_High_Performance_Liquid_Chromatography_High_Resolution_Mass_Spectrometry_and_Post_run_Library_Matching_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/jat/bkw043 DB - PRIME DP - Unbound Medicine ER -