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Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery.
Biomed Pharmacother 2016; 82:393-8BP

Abstract

At present, various scolicidal agents have been used for inactivation of protoscoleces during hydatid cyst surgery, however, they are associated with serious adverse side effects including sclerosing colangititis (biliary tract fibrosis), liver necrosis and methaemoglobinaemia. This investigation was designed to evaluate the chemical composition and in vitro scolicidal effects of Pistacia vera (var. Fandoghi) essential oil against protoscoleces of hydatid cysts and also its toxicity in mice model. The components of the P. vera essential oil were identified by gas chromatography/mass spectroscopy (GC/MS) analysis. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (25-200μl/mL) were used for 5-30min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). In addition, forty male NIH mice were used to determine the acute and sub-acute toxicity of P. vera essential oil for 2 and 14 days, respectively. The main components of P. vera essential oil were limonene (26.21%), α-pinene (18.07%), α-thujene (9.31%) and α-terpinolene (9.28%). Findings of the present study demonstrated that the P. vera essential oil at the concentrations of 100 and 200μl/mL killed 100% protoscoleces after 10 and 5min of exposure, respectively. The LD50 values of intraperitoneal injection of the P. vera essential oil was 2.69ml/kg body weight, and the maximum nonfatal doses were 1.94ml/kg body weight. No significant difference (P>0.05) was observed in the clinical chemistry and hematological parameters following oral administrations of P. vera essential oil at the doses 0.1, 0.2, and 0.4ml/kg for 14 days. The obtained findings demonstrated new chemical composition and promising scolicidal activity of the P. vera with no significant toxicity which might be used as a natural scolicidal agent in hydatid cyst surgery.

Authors+Show Affiliations

Department of Parasitology and Mycology, School of Medicine, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Department of Parasitology and Mycology, School of Medicine, Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman 76169-14111, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman 76169-14111, Iran.Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman 76169-14111, Iran. Electronic address: majid.fasihi@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27470377

Citation

Mahmoudvand, Hossein, et al. "Chemical Composition, Efficacy and Safety of Pistacia Vera (var. Fandoghi) to Inactivate Protoscoleces During Hydatid Cyst Surgery." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 82, 2016, pp. 393-8.
Mahmoudvand H, Kheirandish F, Dezaki ES, et al. Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. Biomed Pharmacother. 2016;82:393-8.
Mahmoudvand, H., Kheirandish, F., Dezaki, E. S., Shamsaddini, S., & Harandi, M. F. (2016). Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 82, pp. 393-8. doi:10.1016/j.biopha.2016.05.012.
Mahmoudvand H, et al. Chemical Composition, Efficacy and Safety of Pistacia Vera (var. Fandoghi) to Inactivate Protoscoleces During Hydatid Cyst Surgery. Biomed Pharmacother. 2016;82:393-8. PubMed PMID: 27470377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. AU - Mahmoudvand,Hossein, AU - Kheirandish,Farnaz, AU - Dezaki,Ebrahim Saedi, AU - Shamsaddini,Saeedeh, AU - Harandi,Majid Fasihi, Y1 - 2016/05/31/ PY - 2016/04/17/received PY - 2016/05/05/revised PY - 2016/05/09/accepted PY - 2016/7/30/entrez PY - 2016/7/30/pubmed PY - 2017/2/1/medline KW - Cystic echinococcosis KW - Hydatid cyst KW - Pistacia KW - Scolicidal KW - Toxicity SP - 393 EP - 8 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 82 N2 - At present, various scolicidal agents have been used for inactivation of protoscoleces during hydatid cyst surgery, however, they are associated with serious adverse side effects including sclerosing colangititis (biliary tract fibrosis), liver necrosis and methaemoglobinaemia. This investigation was designed to evaluate the chemical composition and in vitro scolicidal effects of Pistacia vera (var. Fandoghi) essential oil against protoscoleces of hydatid cysts and also its toxicity in mice model. The components of the P. vera essential oil were identified by gas chromatography/mass spectroscopy (GC/MS) analysis. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (25-200μl/mL) were used for 5-30min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). In addition, forty male NIH mice were used to determine the acute and sub-acute toxicity of P. vera essential oil for 2 and 14 days, respectively. The main components of P. vera essential oil were limonene (26.21%), α-pinene (18.07%), α-thujene (9.31%) and α-terpinolene (9.28%). Findings of the present study demonstrated that the P. vera essential oil at the concentrations of 100 and 200μl/mL killed 100% protoscoleces after 10 and 5min of exposure, respectively. The LD50 values of intraperitoneal injection of the P. vera essential oil was 2.69ml/kg body weight, and the maximum nonfatal doses were 1.94ml/kg body weight. No significant difference (P>0.05) was observed in the clinical chemistry and hematological parameters following oral administrations of P. vera essential oil at the doses 0.1, 0.2, and 0.4ml/kg for 14 days. The obtained findings demonstrated new chemical composition and promising scolicidal activity of the P. vera with no significant toxicity which might be used as a natural scolicidal agent in hydatid cyst surgery. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/27470377/Chemical_composition_efficacy_and_safety_of_Pistacia_vera__var__Fandoghi__to_inactivate_protoscoleces_during_hydatid_cyst_surgery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(16)30502-9 DB - PRIME DP - Unbound Medicine ER -