Tags

Type your tag names separated by a space and hit enter

A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria.
Elife. 2016 07 29; 5E

Abstract

Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens.

Authors+Show Affiliations

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Taian, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27472897

Citation

Yuan, Meng, et al. "A Host Basal Transcription Factor Is a Key Component for Infection of Rice By TALE-carrying Bacteria." ELife, vol. 5, 2016.
Yuan M, Ke Y, Huang R, et al. A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria. Elife. 2016;5.
Yuan, M., Ke, Y., Huang, R., Ma, L., Yang, Z., Chu, Z., Xiao, J., Li, X., & Wang, S. (2016). A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria. ELife, 5. https://doi.org/10.7554/eLife.19605
Yuan M, et al. A Host Basal Transcription Factor Is a Key Component for Infection of Rice By TALE-carrying Bacteria. Elife. 2016 07 29;5 PubMed PMID: 27472897.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A host basal transcription factor is a key component for infection of rice by TALE-carrying bacteria. AU - Yuan,Meng, AU - Ke,Yinggen, AU - Huang,Renyan, AU - Ma,Ling, AU - Yang,Zeyu, AU - Chu,Zhaohui, AU - Xiao,Jinghua, AU - Li,Xianghua, AU - Wang,Shiping, Y1 - 2016/07/29/ PY - 2016/07/13/received PY - 2016/07/21/accepted PY - 2016/7/30/entrez PY - 2016/7/30/pubmed PY - 2017/11/29/medline KW - Xanthomonas KW - plant biology KW - rice KW - transcription factor IIA JF - eLife JO - Elife VL - 5 N2 - Transcription activator-like effectors (TALEs) are sequence-specific DNA binding proteins found in a range of plant pathogenic bacteria, where they play important roles in host-pathogen interactions. However, it has been unclear how TALEs, after they have been injected into the host cells, activate transcription of host genes required for infection success. Here, we show that the basal transcription factor IIA gamma subunit TFIIAγ5 from rice is a key component for infection by the TALE-carrying bacterium Xanthomonas oryzae pv. oryzae, the causal agent for bacterial blight. Direct interaction of several TALEs with TFIIAγ5 is required for activation of disease susceptibility genes. Conversely, reduced expression of the TFIIAγ5 host gene limits the induction of susceptibility genes and thus decreases bacterial blight symptoms. Suppression or mutation of TFIIAγ5 can also reduce bacterial streak, another devastating disease of rice caused by TALE-carrying X. oryzae pv. oryzicola. These results have important implications for formulating a widely applicable strategy with which to improve resistance of plants to TALE-carrying pathogens. SN - 2050-084X UR - https://www.unboundmedicine.com/medline/citation/27472897/A_host_basal_transcription_factor_is_a_key_component_for_infection_of_rice_by_TALE_carrying_bacteria_ L2 - https://doi.org/10.7554/eLife.19605 DB - PRIME DP - Unbound Medicine ER -