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Protective Effect of Ginsenoside Rg1 on Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway.
Biol Pharm Bull. 2016; 39(8):1284-92.BP

Abstract

Idiopathic pulmonary fibrosis (IPF) is a progressive disease with poor prognosis and high mortality rate. Panax Notoginseng Saponins (PNS), extracted from Panax Notoginseng as a traditional Asian medicine, displayed a significant anti-fibrosis effect in liver and lung. However, whether Ginsenoside Rg1 (Rg1), an important and active ingredient of PNS, exerts anti-fibrotic activity on IPF still remain unclear. In this study, we investigated the effect of Rg1 on bleomycin-induced pulmonary fibrosis in rats. Bleomycin (5 mg/kg body weight) was intratracheally administrated to male rats. Rg1 (18, 36 and 72 mg/kg) was orally administered on the next day after bleomycin. Lungs were harvested at day 7 and 28 for the further experiments. Histological analysis revealed that bleomycin successfully induced pulmonary fibrosis, and that Rg1 restored the histological alteration of bleomycin-induced pulmonary fibrosis (PF), significantly decreased lung coefficient, scores of alveolitis, scores of PF as well as contents of alpha smooth muscle actin (α-SMA) and hydroxyproline (Hyp) in a dose-dependent manner in PF rats. Moreover, Rg1 increased the expression levels of Caveolin-1 (Cav-1) mRNA and protein, lowered the expression of transforming growth factor-β1 (TGF-β1) mRNA and protein in the lung tissues of PF rats. These data suggest that Rg1 exhibits protective effect against bleomycin-induced PF in rats, which is potentially associated with the down-regulation of TGF-β1 and up-regulation of Cav-1.

Authors+Show Affiliations

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27476938

Citation

Zhan, Heqin, et al. "Protective Effect of Ginsenoside Rg1 On Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway." Biological & Pharmaceutical Bulletin, vol. 39, no. 8, 2016, pp. 1284-92.
Zhan H, Huang F, Ma W, et al. Protective Effect of Ginsenoside Rg1 on Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway. Biol Pharm Bull. 2016;39(8):1284-92.
Zhan, H., Huang, F., Ma, W., Zhao, Z., Zhang, H., & Zhang, C. (2016). Protective Effect of Ginsenoside Rg1 on Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway. Biological & Pharmaceutical Bulletin, 39(8), 1284-92. https://doi.org/10.1248/bpb.b16-00046
Zhan H, et al. Protective Effect of Ginsenoside Rg1 On Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway. Biol Pharm Bull. 2016;39(8):1284-92. PubMed PMID: 27476938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective Effect of Ginsenoside Rg1 on Bleomycin-Induced Pulmonary Fibrosis in Rats: Involvement of Caveolin-1 and TGF-β1 Signal Pathway. AU - Zhan,Heqin, AU - Huang,Feng, AU - Ma,Wenzhuo, AU - Zhao,Zhenghang, AU - Zhang,Haifang, AU - Zhang,Chong, PY - 2016/8/2/entrez PY - 2016/8/2/pubmed PY - 2017/1/28/medline SP - 1284 EP - 92 JF - Biological & pharmaceutical bulletin JO - Biol Pharm Bull VL - 39 IS - 8 N2 - Idiopathic pulmonary fibrosis (IPF) is a progressive disease with poor prognosis and high mortality rate. Panax Notoginseng Saponins (PNS), extracted from Panax Notoginseng as a traditional Asian medicine, displayed a significant anti-fibrosis effect in liver and lung. However, whether Ginsenoside Rg1 (Rg1), an important and active ingredient of PNS, exerts anti-fibrotic activity on IPF still remain unclear. In this study, we investigated the effect of Rg1 on bleomycin-induced pulmonary fibrosis in rats. Bleomycin (5 mg/kg body weight) was intratracheally administrated to male rats. Rg1 (18, 36 and 72 mg/kg) was orally administered on the next day after bleomycin. Lungs were harvested at day 7 and 28 for the further experiments. Histological analysis revealed that bleomycin successfully induced pulmonary fibrosis, and that Rg1 restored the histological alteration of bleomycin-induced pulmonary fibrosis (PF), significantly decreased lung coefficient, scores of alveolitis, scores of PF as well as contents of alpha smooth muscle actin (α-SMA) and hydroxyproline (Hyp) in a dose-dependent manner in PF rats. Moreover, Rg1 increased the expression levels of Caveolin-1 (Cav-1) mRNA and protein, lowered the expression of transforming growth factor-β1 (TGF-β1) mRNA and protein in the lung tissues of PF rats. These data suggest that Rg1 exhibits protective effect against bleomycin-induced PF in rats, which is potentially associated with the down-regulation of TGF-β1 and up-regulation of Cav-1. SN - 1347-5215 UR - https://www.unboundmedicine.com/medline/citation/27476938/Protective_Effect_of_Ginsenoside_Rg1_on_Bleomycin_Induced_Pulmonary_Fibrosis_in_Rats:_Involvement_of_Caveolin_1_and_TGF_β1_Signal_Pathway_ L2 - https://dx.doi.org/10.1248/bpb.b16-00046 DB - PRIME DP - Unbound Medicine ER -