Tags

Type your tag names separated by a space and hit enter

Microarray-based annotation of the gut transcriptome of the migratory locust, Locusta migratoria.
Insect Mol Biol. 2016 12; 25(6):745-756.IM

Abstract

The migratory locust, Locusta migratoria, is a serious agricultural pest and important insect model in the study of insect digestion and feeding behaviour. The gut is one of the primary interfaces between the insect and its environment. Nevertheless, knowledge on the gut transcriptome of L. migratoria is still very limited. Here, 48 802 expressed sequence tags were extracted from publicly available databases and their expression in larval gut and/or brain tissue was determined using microarray hybridization. Our data show 2765 transcripts predominantly or exclusively expressed in the gut. Many transcripts had putative functions closely related to the physiological functions of the gut as a muscular digestive organ and as the first barrier against microorganisms and a wide range of toxins. By means of a ranking procedure based on the relative signal intensity, we estimated 15% of the transcripts to show high expression levels, the highest belonging to diverse digestive enzymes and muscle-related proteins. We also found evidence for very high expression of an allergen protein, which could have important implications, as locusts form a traditional food source in various parts of the world, and were also recently added to the list of insects fit for human consumption in Europe. Interestingly, many highly expressed sequences have as yet unknown functions. Taken together, the present data provide significant insight into locust larval gut physiology, and will be valuable for future studies on the insect gut.

Authors+Show Affiliations

Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Leuven, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Leuven, Belgium.Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Antwerpen, Belgium. Zebrafishlab, Veterinary Physiology and Biochemistry Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.Systemic Physiological and Ecotoxicological Research (SPHERE), Department of Biology, University of Antwerp, Antwerpen, Belgium. Zebrafishlab, Veterinary Physiology and Biochemistry Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium.Department of Animal Physiology and Neurobiology, Zoological Institute KU Leuven, Leuven, Belgium.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27479692

Citation

Spit, J, et al. "Microarray-based Annotation of the Gut Transcriptome of the Migratory Locust, Locusta Migratoria." Insect Molecular Biology, vol. 25, no. 6, 2016, pp. 745-756.
Spit J, Badisco L, Vergauwen L, et al. Microarray-based annotation of the gut transcriptome of the migratory locust, Locusta migratoria. Insect Mol Biol. 2016;25(6):745-756.
Spit, J., Badisco, L., Vergauwen, L., Knapen, D., & Vanden Broeck, J. (2016). Microarray-based annotation of the gut transcriptome of the migratory locust, Locusta migratoria. Insect Molecular Biology, 25(6), 745-756. https://doi.org/10.1111/imb.12258
Spit J, et al. Microarray-based Annotation of the Gut Transcriptome of the Migratory Locust, Locusta Migratoria. Insect Mol Biol. 2016;25(6):745-756. PubMed PMID: 27479692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microarray-based annotation of the gut transcriptome of the migratory locust, Locusta migratoria. AU - Spit,J, AU - Badisco,L, AU - Vergauwen,L, AU - Knapen,D, AU - Vanden Broeck,J, Y1 - 2016/08/01/ PY - 2016/8/2/pubmed PY - 2017/9/7/medline PY - 2016/8/2/entrez KW - allergen KW - digestion KW - gut KW - insect KW - locust KW - transcriptome SP - 745 EP - 756 JF - Insect molecular biology JO - Insect Mol Biol VL - 25 IS - 6 N2 - The migratory locust, Locusta migratoria, is a serious agricultural pest and important insect model in the study of insect digestion and feeding behaviour. The gut is one of the primary interfaces between the insect and its environment. Nevertheless, knowledge on the gut transcriptome of L. migratoria is still very limited. Here, 48 802 expressed sequence tags were extracted from publicly available databases and their expression in larval gut and/or brain tissue was determined using microarray hybridization. Our data show 2765 transcripts predominantly or exclusively expressed in the gut. Many transcripts had putative functions closely related to the physiological functions of the gut as a muscular digestive organ and as the first barrier against microorganisms and a wide range of toxins. By means of a ranking procedure based on the relative signal intensity, we estimated 15% of the transcripts to show high expression levels, the highest belonging to diverse digestive enzymes and muscle-related proteins. We also found evidence for very high expression of an allergen protein, which could have important implications, as locusts form a traditional food source in various parts of the world, and were also recently added to the list of insects fit for human consumption in Europe. Interestingly, many highly expressed sequences have as yet unknown functions. Taken together, the present data provide significant insight into locust larval gut physiology, and will be valuable for future studies on the insect gut. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/27479692/Microarray_based_annotation_of_the_gut_transcriptome_of_the_migratory_locust_Locusta_migratoria_ DB - PRIME DP - Unbound Medicine ER -