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Lutein attenuates oxidative stress markers and ameliorates glucose homeostasis through polyol pathway in heart and kidney of STZ-induced hyperglycemic rat model.
Eur J Nutr. 2017 Dec; 56(8):2475-2485.EJ

Abstract

PURPOSE

Lutein's role on chronic hyperglycemia-induced oxidative stress and associated glucose homeostasis in heart and kidney is limited. Purpose of the study is to investigate the effect of lutein on cardiac and renal polyol pathway enzymes and oxidative stress markers under hyperglycemia-induced oxidative stress condition using streptozotocin (STZ)-injected rat model.

METHODS

STZ-induced hyperglycemic (fasting blood glucose ≥11 mM) male Wistar rats were divided into two groups (n = 11/group). Group 1 received micellar lutein (39 nmol/day/rat) and group 2 (negative control) received micelle without lutein for 8 weeks. A separate group (no STZ injected) served as a positive control (n = 11/group). Oral glucose tolerance test (OGTT), biweekly urine glucose and activities of aldose reductase (AR) and sorbitol dehydrogenase (SDH) enzymes were assessed. Activities of antioxidant enzymes and antioxidant level were also evaluated.

RESULTS

Lutein-administered hyperglycemic rats showed better glucose tolerance as evidenced with OGTT and biweekly urine glucose when compared to negative control. Activities of AR and SDH were decreased in heart and kidney of lutein-fed hyperglycemic rats. Also, they had significantly (p < 0.05) decreased malondialdehyde levels (66, 34, and 33 %) and increased reduced glutathione level (81, 18 and 92 %) in serum, heart and kidney, respectively. Altered antioxidant enzyme activities such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase were also affected in serum, heart and kidney of lutein-fed diabetic group.

CONCLUSION

Lutein prevented cardiac and renal injury in STZ-induced hyperglycemic rats due to potential amelioration of altered activities in polyol pathway and oxidative stress markers.

Authors+Show Affiliations

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India.Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India.Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India. baskaranv@cftri.res.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27488609

Citation

Sharavana, Gurunathan, et al. "Lutein Attenuates Oxidative Stress Markers and Ameliorates Glucose Homeostasis Through Polyol Pathway in Heart and Kidney of STZ-induced Hyperglycemic Rat Model." European Journal of Nutrition, vol. 56, no. 8, 2017, pp. 2475-2485.
Sharavana G, Joseph GS, Baskaran V. Lutein attenuates oxidative stress markers and ameliorates glucose homeostasis through polyol pathway in heart and kidney of STZ-induced hyperglycemic rat model. Eur J Nutr. 2017;56(8):2475-2485.
Sharavana, G., Joseph, G. S., & Baskaran, V. (2017). Lutein attenuates oxidative stress markers and ameliorates glucose homeostasis through polyol pathway in heart and kidney of STZ-induced hyperglycemic rat model. European Journal of Nutrition, 56(8), 2475-2485. https://doi.org/10.1007/s00394-016-1283-0
Sharavana G, Joseph GS, Baskaran V. Lutein Attenuates Oxidative Stress Markers and Ameliorates Glucose Homeostasis Through Polyol Pathway in Heart and Kidney of STZ-induced Hyperglycemic Rat Model. Eur J Nutr. 2017;56(8):2475-2485. PubMed PMID: 27488609.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lutein attenuates oxidative stress markers and ameliorates glucose homeostasis through polyol pathway in heart and kidney of STZ-induced hyperglycemic rat model. AU - Sharavana,Gurunathan, AU - Joseph,G S, AU - Baskaran,Vallikannan, Y1 - 2016/08/03/ PY - 2016/04/20/received PY - 2016/07/25/accepted PY - 2016/8/5/pubmed PY - 2018/7/13/medline PY - 2016/8/5/entrez KW - Diabetes KW - Lutein KW - Nephropathy KW - Oxidative stress KW - Polyol pathway SP - 2475 EP - 2485 JF - European journal of nutrition JO - Eur J Nutr VL - 56 IS - 8 N2 - PURPOSE: Lutein's role on chronic hyperglycemia-induced oxidative stress and associated glucose homeostasis in heart and kidney is limited. Purpose of the study is to investigate the effect of lutein on cardiac and renal polyol pathway enzymes and oxidative stress markers under hyperglycemia-induced oxidative stress condition using streptozotocin (STZ)-injected rat model. METHODS: STZ-induced hyperglycemic (fasting blood glucose ≥11 mM) male Wistar rats were divided into two groups (n = 11/group). Group 1 received micellar lutein (39 nmol/day/rat) and group 2 (negative control) received micelle without lutein for 8 weeks. A separate group (no STZ injected) served as a positive control (n = 11/group). Oral glucose tolerance test (OGTT), biweekly urine glucose and activities of aldose reductase (AR) and sorbitol dehydrogenase (SDH) enzymes were assessed. Activities of antioxidant enzymes and antioxidant level were also evaluated. RESULTS: Lutein-administered hyperglycemic rats showed better glucose tolerance as evidenced with OGTT and biweekly urine glucose when compared to negative control. Activities of AR and SDH were decreased in heart and kidney of lutein-fed hyperglycemic rats. Also, they had significantly (p < 0.05) decreased malondialdehyde levels (66, 34, and 33 %) and increased reduced glutathione level (81, 18 and 92 %) in serum, heart and kidney, respectively. Altered antioxidant enzyme activities such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione transferase were also affected in serum, heart and kidney of lutein-fed diabetic group. CONCLUSION: Lutein prevented cardiac and renal injury in STZ-induced hyperglycemic rats due to potential amelioration of altered activities in polyol pathway and oxidative stress markers. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/27488609/Lutein_attenuates_oxidative_stress_markers_and_ameliorates_glucose_homeostasis_through_polyol_pathway_in_heart_and_kidney_of_STZ_induced_hyperglycemic_rat_model_ L2 - https://dx.doi.org/10.1007/s00394-016-1283-0 DB - PRIME DP - Unbound Medicine ER -