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Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis.
J Exp Bot. 2016 10; 67(18):5363-5380.JE

Abstract

A plant cuticle forms a hydrophobic layer covering plant organs, and plays an important role in plant development and protection from environmental stresses. We examined epicuticular structure, composition, and a MYB-based regulatory network in two Australian wheat cultivars, RAC875 and Kukri, with contrasting cuticle appearance (glaucousness) and drought tolerance. Metabolomics and microscopic analyses of epicuticular waxes revealed that the content of β-diketones was the major compositional and structural difference between RAC875 and Kukri. The content of β-diketones remained the same while those of alkanes and primary alcohols were increased by drought in both cultivars, suggesting that the interplay of all components rather than a single one defines the difference in drought tolerance between cultivars. Six wheat genes encoding MYB transcription factors (TFs) were cloned; four of them were regulated in flag leaves of both cultivars by rapid dehydration and/or slowly developing cyclic drought. The involvement of selected MYB TFs in the regulation of cuticle biosynthesis was confirmed by a transient expression assay in wheat cell culture, using the promoters of wheat genes encoding cuticle biosynthesis-related enzymes and the SHINE1 (SHN1) TF. Two functional MYB-responsive elements, specifically recognized by TaMYB74 but not by other MYB TFs, were localized in the TdSHN1 promoter. Protein structural determinants underlying the binding specificity of TaMYB74 for functional DNA cis-elements were defined, using 3D protein molecular modelling. A scheme, linking drought-induced expression of the investigated TFs with downstream genes that participate in the synthesis of cuticle components, is proposed.

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Authors+Show Affiliations

Bi H
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Luang S
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Li Y
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Bazanova N
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Morran S
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Song Z
W.M.Keck Metabolomics Research Laboratory, Iowa State University, Ames, IA 50011, USA.
Perera MA
W.M.Keck Metabolomics Research Laboratory, Iowa State University, Ames, IA 50011, USA.
Hrmova M
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia maria.hrmova@adelaide.edu.au.
Borisjuk N
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.
Lopato S
Australian Centre for Plant Functional Genomics, School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, South Australia 5064, Australia.

MeSH

DehydrationGene Expression Regulation, PlantMicroscopy, Electron, ScanningPlant LeavesPlant ProteinsTranscription FactorsTriticumWaxes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27489236

Citation

Bi, Huihui, et al. "Identification and Characterization of Wheat Drought-responsive MYB Transcription Factors Involved in the Regulation of Cuticle Biosynthesis." Journal of Experimental Botany, vol. 67, no. 18, 2016, pp. 5363-5380.
Bi H, Luang S, Li Y, et al. Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. J Exp Bot. 2016;67(18):5363-5380.
Bi, H., Luang, S., Li, Y., Bazanova, N., Morran, S., Song, Z., Perera, M. A., Hrmova, M., Borisjuk, N., & Lopato, S. (2016). Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. Journal of Experimental Botany, 67(18), 5363-5380.
Bi H, et al. Identification and Characterization of Wheat Drought-responsive MYB Transcription Factors Involved in the Regulation of Cuticle Biosynthesis. J Exp Bot. 2016;67(18):5363-5380. PubMed PMID: 27489236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. AU - Bi,Huihui, AU - Luang,Sukanya, AU - Li,Yuan, AU - Bazanova,Natalia, AU - Morran,Sarah, AU - Song,Zhihong, AU - Perera,M Ann, AU - Hrmova,Maria, AU - Borisjuk,Nikolai, AU - Lopato,Sergiy, Y1 - 2016/08/03/ PY - 2016/8/5/pubmed PY - 2017/11/9/medline PY - 2016/8/5/entrez KW - Abiotic stress KW - MYB and SHINE1 transcription factors KW - cuticle KW - drought KW - molecular model KW - water deficit KW - wax KW - wheat. KW - β-diketone SP - 5363 EP - 5380 JF - Journal of experimental botany JO - J Exp Bot VL - 67 IS - 18 N2 - A plant cuticle forms a hydrophobic layer covering plant organs, and plays an important role in plant development and protection from environmental stresses. We examined epicuticular structure, composition, and a MYB-based regulatory network in two Australian wheat cultivars, RAC875 and Kukri, with contrasting cuticle appearance (glaucousness) and drought tolerance. Metabolomics and microscopic analyses of epicuticular waxes revealed that the content of β-diketones was the major compositional and structural difference between RAC875 and Kukri. The content of β-diketones remained the same while those of alkanes and primary alcohols were increased by drought in both cultivars, suggesting that the interplay of all components rather than a single one defines the difference in drought tolerance between cultivars. Six wheat genes encoding MYB transcription factors (TFs) were cloned; four of them were regulated in flag leaves of both cultivars by rapid dehydration and/or slowly developing cyclic drought. The involvement of selected MYB TFs in the regulation of cuticle biosynthesis was confirmed by a transient expression assay in wheat cell culture, using the promoters of wheat genes encoding cuticle biosynthesis-related enzymes and the SHINE1 (SHN1) TF. Two functional MYB-responsive elements, specifically recognized by TaMYB74 but not by other MYB TFs, were localized in the TdSHN1 promoter. Protein structural determinants underlying the binding specificity of TaMYB74 for functional DNA cis-elements were defined, using 3D protein molecular modelling. A scheme, linking drought-induced expression of the investigated TFs with downstream genes that participate in the synthesis of cuticle components, is proposed. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/27489236/Identification_and_characterization_of_wheat_drought_responsive_MYB_transcription_factors_involved_in_the_regulation_of_cuticle_biosynthesis_ DB - PRIME DP - Unbound Medicine ER -