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A novel screening method for 64 new psychoactive substances and 5 amphetamines in blood by LC-MS/MS and application to real cases.
J Pharm Biomed Anal. 2016 Sep 10; 129:441-449.JP

Abstract

Identification and quantification of new psychoactive substances (NPS), both in biological and non-biological samples, represent a hard challenge for forensic toxicologists. NPS are increasingly emerging on illegal drug market. Many cases of co-consumption of NPS and other substances have also been reported. Hence, the development of analytical methods aiming at the detection of a broad-spectrum of compounds (NPS and "traditional" drugs) could be helpful. In this paper, a fully validated screening method in blood for the simultaneous detection of 69 substances, including 64 NPS (28 synthetic cannabinoids, 19 synthetic cathinones, 5 phenethylamines, 3 indanes, 2 piperazines, 2 tryptamines, 2 phencyclidine, methoxetamine, ketamine and its metabolite) and 5 amphetamines (amphetamine, methamphetamine, MDMA, MDA, 3,4-methylenedioxy-N-ethylamphetamine - MDEA-) by a dynamic multiple reaction monitoring analysis through liquid chromatography - tandem mass spectrometry (LC-MS/MS) is described. This method is very fast, easy to perform and cheap as it only requires the deproteinization of 200μL of blood sample with acetonitrile. The chromatographic separation is achieved with a C18 column. The analysis is very sensitive, with limits of quantification ranging from 0.1 to 0.5ng/mL. The method is linear from 1 to 100ng/mL and the coefficient of determination (R(2)) was always above 0.9900. Precision and accuracy were acceptable at any quality control level and recovery efficiency range was 72-110%. Matrix effects did not negatively affect the analytical sensitivity. This method was successfully applied to three real cases, allowing identification and quantification of: mephedrone and methamphetamine (post-mortem); ketamine, MDMA and MDA (post-mortem); AB-FUBINACA (ante-mortem).

Authors+Show Affiliations

Department of Health Science, University of Florence, Italy.Unit of Forensic Toxicology (UoFT) - Department of Anatomical Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Italy. Electronic address: fra.busardo@libero.it.Department of Health Science, University of Florence, Italy.Unit of Forensic Toxicology (UoFT) - Department of Anatomical Histological, Forensic and Orthopaedic Sciences, Sapienza University of Rome, Italy.Department of Health Science, University of Florence, Italy.Department of Health Science, University of Florence, Italy.Department of Health Science, University of Florence, Italy.Department of Health Science, University of Florence, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27490334

Citation

Vaiano, Fabio, et al. "A Novel Screening Method for 64 New Psychoactive Substances and 5 Amphetamines in Blood By LC-MS/MS and Application to Real Cases." Journal of Pharmaceutical and Biomedical Analysis, vol. 129, 2016, pp. 441-449.
Vaiano F, Busardò FP, Palumbo D, et al. A novel screening method for 64 new psychoactive substances and 5 amphetamines in blood by LC-MS/MS and application to real cases. J Pharm Biomed Anal. 2016;129:441-449.
Vaiano, F., Busardò, F. P., Palumbo, D., Kyriakou, C., Fioravanti, A., Catalani, V., Mari, F., & Bertol, E. (2016). A novel screening method for 64 new psychoactive substances and 5 amphetamines in blood by LC-MS/MS and application to real cases. Journal of Pharmaceutical and Biomedical Analysis, 129, 441-449. https://doi.org/10.1016/j.jpba.2016.07.009
Vaiano F, et al. A Novel Screening Method for 64 New Psychoactive Substances and 5 Amphetamines in Blood By LC-MS/MS and Application to Real Cases. J Pharm Biomed Anal. 2016 Sep 10;129:441-449. PubMed PMID: 27490334.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel screening method for 64 new psychoactive substances and 5 amphetamines in blood by LC-MS/MS and application to real cases. AU - Vaiano,Fabio, AU - Busardò,Francesco P, AU - Palumbo,Diego, AU - Kyriakou,Chrystalla, AU - Fioravanti,Alessia, AU - Catalani,Valeria, AU - Mari,Francesco, AU - Bertol,Elisabetta, Y1 - 2016/07/07/ PY - 2016/05/13/received PY - 2016/07/03/revised PY - 2016/07/07/accepted PY - 2016/8/5/entrez PY - 2016/8/5/pubmed PY - 2017/5/4/medline KW - Amphetamines KW - Blood screening KW - LC–MS/MS KW - NPS KW - NPS intoxication cases SP - 441 EP - 449 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 129 N2 - Identification and quantification of new psychoactive substances (NPS), both in biological and non-biological samples, represent a hard challenge for forensic toxicologists. NPS are increasingly emerging on illegal drug market. Many cases of co-consumption of NPS and other substances have also been reported. Hence, the development of analytical methods aiming at the detection of a broad-spectrum of compounds (NPS and "traditional" drugs) could be helpful. In this paper, a fully validated screening method in blood for the simultaneous detection of 69 substances, including 64 NPS (28 synthetic cannabinoids, 19 synthetic cathinones, 5 phenethylamines, 3 indanes, 2 piperazines, 2 tryptamines, 2 phencyclidine, methoxetamine, ketamine and its metabolite) and 5 amphetamines (amphetamine, methamphetamine, MDMA, MDA, 3,4-methylenedioxy-N-ethylamphetamine - MDEA-) by a dynamic multiple reaction monitoring analysis through liquid chromatography - tandem mass spectrometry (LC-MS/MS) is described. This method is very fast, easy to perform and cheap as it only requires the deproteinization of 200μL of blood sample with acetonitrile. The chromatographic separation is achieved with a C18 column. The analysis is very sensitive, with limits of quantification ranging from 0.1 to 0.5ng/mL. The method is linear from 1 to 100ng/mL and the coefficient of determination (R(2)) was always above 0.9900. Precision and accuracy were acceptable at any quality control level and recovery efficiency range was 72-110%. Matrix effects did not negatively affect the analytical sensitivity. This method was successfully applied to three real cases, allowing identification and quantification of: mephedrone and methamphetamine (post-mortem); ketamine, MDMA and MDA (post-mortem); AB-FUBINACA (ante-mortem). SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/27490334/A_novel_screening_method_for_64_new_psychoactive_substances_and_5_amphetamines_in_blood_by_LC_MS/MS_and_application_to_real_cases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(16)30381-8 DB - PRIME DP - Unbound Medicine ER -