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RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi.
Future Microbiol. 2016 08; 11:1011-24.FM

Abstract

AIM

To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella.

MATERIALS & METHODS

Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress.

RESULTS

SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains.

CONCLUSION

RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2.

Authors+Show Affiliations

Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, PR China. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, Jiangsu 210093, PR China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27492279

Citation

Zhang, Haifang, et al. "RpoE Promotes Invasion and Intracellular Survival By Regulating SPI-1 and SPI-2 in Salmonella Enterica Serovar Typhi." Future Microbiology, vol. 11, 2016, pp. 1011-24.
Zhang H, Jia Y, Xie X, et al. RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi. Future Microbiol. 2016;11:1011-24.
Zhang, H., Jia, Y., Xie, X., Wang, M., Zheng, Y., Xu, S., Zhang, W., Wang, Q., Huang, X., & Du, H. (2016). RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi. Future Microbiology, 11, 1011-24. https://doi.org/10.2217/fmb.16.19
Zhang H, et al. RpoE Promotes Invasion and Intracellular Survival By Regulating SPI-1 and SPI-2 in Salmonella Enterica Serovar Typhi. Future Microbiol. 2016;11:1011-24. PubMed PMID: 27492279.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RpoE promotes invasion and intracellular survival by regulating SPI-1 and SPI-2 in Salmonella enterica serovar Typhi. AU - Zhang,Haifang, AU - Jia,Yanwei, AU - Xie,Xiaofang, AU - Wang,Min, AU - Zheng,Yi, AU - Xu,Shungao, AU - Zhang,Wei, AU - Wang,Qiang, AU - Huang,Xinxiang, AU - Du,Hong, Y1 - 2016/08/05/ PY - 2016/8/6/entrez PY - 2016/8/6/pubmed PY - 2017/7/14/medline KW - RpoE KW - SPI-1 KW - SPI-2 KW - Salmonella enterica serovar Typhi KW - SipC KW - hyperosmotic stress SP - 1011 EP - 24 JF - Future microbiology JO - Future Microbiol VL - 11 N2 - AIM: To demonstrate the role of RpoE during the later stage of hyperosmotic stress in Salmonella. MATERIALS & METHODS: Expressions of SPI-1 and SPI-2 under hyperosmotic stress for 120 min were investigated by a microarray, and the invasion and intracellular survival of wild-type and ΔrpoE strains were compared. The global differential expression of bacterial proteins between the wild-type and ΔrpoE strains was examined after 120 min of hyperosmotic stress. RESULTS: SPI-1 and SPI-2 were repressed, and the invasion and intracellular survival were defected in the ΔrpoE strain. Thirteen bacterial-associated proteins and 11 secreted proteins differed significantly between the wild-type and ΔrpoE strains. CONCLUSION: RpoE may promote invasion and intracellular survival by regulating the expression of SPI-1 and SPI-2. SN - 1746-0921 UR - https://www.unboundmedicine.com/medline/citation/27492279/RpoE_promotes_invasion_and_intracellular_survival_by_regulating_SPI_1_and_SPI_2_in_Salmonella_enterica_serovar_Typhi_ L2 - https://www.futuremedicine.com/doi/10.2217/fmb.16.19?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -