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Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda.
BMC Microbiol 2016; 16(1):178BM

Abstract

BACKGROUND

Enterotoxigenic E. coli (ETEC) significantly contribute to diarrhea in piglets and weaners. The smallholder pig producers in Uganda identified diarrhea as one of the major problems especially in piglets. The aim of this study was to; i) characterize the virulence factors of E. coli strains isolated from diarrheic and non-diarrheic suckling piglets and weaners from smallholder herds in northern and eastern Uganda and ii) identify and describe the post-mortem picture of ETEC infection in severely diarrheic piglets. Rectal swab samples were collected from 83 piglets and weaners in 20 herds and isolated E. coli were characterized by PCR, serotyping and hemolysis.

RESULTS

The E. coli strains carried genes for the heat stable toxins STa, STb and EAST1 and adhesins F4 and AIDA-I. The genes for the heat labile toxin LT and adhesins F5, F6, F18 and F41 were not detected in any of the E. coli isolates. Where the serogroup could be identified, E. coli isolates from the same diarrheic pig belonged to the same serogroup. The prevalence of EAST1, STb, Stx2e, STa, AIDA-I, and F4 in the E. coli isolates from suckling piglets and weaners (diarrheic and non-diarrheic combined) was 29, 26.5, 2.4, 1.2, 16, and 8.4 %, respectively. However the prevalence of F4 and AIDA-I in E. coli from diarrheic suckling piglets alone was 22.2 and 20 %, respectively. There was no significant difference in the prevalence of the individual virulence factors in E. coli from the diarrheic and non-diarrheic pigs (p > 0.05). The main ETEC strains isolated from diarrheic and non-diarrheic pigs included F4/STb/EAST1 (7.2 %), F4/STb (1.2 %), AIDA/STb/EAST1 (8 %) and AIDA/STb (8 %). At post-mortem, two diarrheic suckling piglets carrying ETEC showed intact intestinal villi, enterocytes and brush border but with a layer of cells attached to the brush border, suggestive of ETEC infections.

CONCLUSION

This study has shown that the F4 fimbriae is the most predominant in E. coli from diarrheic piglets in the study area and therefore an F4-based vaccine should be considered one of the preventive measures for controlling ETEC infections in the piglets in northern and eastern Uganda.

Authors+Show Affiliations

College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda. ikwap@covab.mak.ac.ug.Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7070, SE-750 07, Uppsala, Sweden.Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, P.O. Box 7070, SE-750 07, Uppsala, Sweden.College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.National Veterinary Institute, Uppsala, 751 89, Sweden.National Veterinary Institute, Uppsala, 751 89, Sweden.College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, P. O. Box 7062, Kampala, Uganda.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27496201

Citation

Ikwap, Kokas, et al. "Prevalence of Adhesin and Toxin Genes in E. Coli Strains Isolated From Diarrheic and Non-diarrheic Pigs From Smallholder Herds in Northern and Eastern Uganda." BMC Microbiology, vol. 16, no. 1, 2016, p. 178.
Ikwap K, Larsson J, Jacobson M, et al. Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda. BMC Microbiol. 2016;16(1):178.
Ikwap, K., Larsson, J., Jacobson, M., Owiny, D. O., Nasinyama, G. W., Nabukenya, I., ... Erume, J. (2016). Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda. BMC Microbiology, 16(1), p. 178. doi:10.1186/s12866-016-0796-2.
Ikwap K, et al. Prevalence of Adhesin and Toxin Genes in E. Coli Strains Isolated From Diarrheic and Non-diarrheic Pigs From Smallholder Herds in Northern and Eastern Uganda. BMC Microbiol. 2016 08 5;16(1):178. PubMed PMID: 27496201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of adhesin and toxin genes in E. coli strains isolated from diarrheic and non-diarrheic pigs from smallholder herds in northern and eastern Uganda. AU - Ikwap,Kokas, AU - Larsson,Jenny, AU - Jacobson,Magdalena, AU - Owiny,David Okello, AU - Nasinyama,George William, AU - Nabukenya,Immaculate, AU - Mattsson,Sigbrit, AU - Aspan,Anna, AU - Erume,Joseph, Y1 - 2016/08/05/ PY - 2016/03/26/received PY - 2016/08/01/accepted PY - 2016/8/7/entrez PY - 2016/8/9/pubmed PY - 2017/6/1/medline KW - AIDA-I KW - Escherichia coli KW - F4 KW - Hemolytic KW - Piglets SP - 178 EP - 178 JF - BMC microbiology JO - BMC Microbiol. VL - 16 IS - 1 N2 - BACKGROUND: Enterotoxigenic E. coli (ETEC) significantly contribute to diarrhea in piglets and weaners. The smallholder pig producers in Uganda identified diarrhea as one of the major problems especially in piglets. The aim of this study was to; i) characterize the virulence factors of E. coli strains isolated from diarrheic and non-diarrheic suckling piglets and weaners from smallholder herds in northern and eastern Uganda and ii) identify and describe the post-mortem picture of ETEC infection in severely diarrheic piglets. Rectal swab samples were collected from 83 piglets and weaners in 20 herds and isolated E. coli were characterized by PCR, serotyping and hemolysis. RESULTS: The E. coli strains carried genes for the heat stable toxins STa, STb and EAST1 and adhesins F4 and AIDA-I. The genes for the heat labile toxin LT and adhesins F5, F6, F18 and F41 were not detected in any of the E. coli isolates. Where the serogroup could be identified, E. coli isolates from the same diarrheic pig belonged to the same serogroup. The prevalence of EAST1, STb, Stx2e, STa, AIDA-I, and F4 in the E. coli isolates from suckling piglets and weaners (diarrheic and non-diarrheic combined) was 29, 26.5, 2.4, 1.2, 16, and 8.4 %, respectively. However the prevalence of F4 and AIDA-I in E. coli from diarrheic suckling piglets alone was 22.2 and 20 %, respectively. There was no significant difference in the prevalence of the individual virulence factors in E. coli from the diarrheic and non-diarrheic pigs (p > 0.05). The main ETEC strains isolated from diarrheic and non-diarrheic pigs included F4/STb/EAST1 (7.2 %), F4/STb (1.2 %), AIDA/STb/EAST1 (8 %) and AIDA/STb (8 %). At post-mortem, two diarrheic suckling piglets carrying ETEC showed intact intestinal villi, enterocytes and brush border but with a layer of cells attached to the brush border, suggestive of ETEC infections. CONCLUSION: This study has shown that the F4 fimbriae is the most predominant in E. coli from diarrheic piglets in the study area and therefore an F4-based vaccine should be considered one of the preventive measures for controlling ETEC infections in the piglets in northern and eastern Uganda. SN - 1471-2180 UR - https://www.unboundmedicine.com/medline/citation/27496201/Prevalence_of_adhesin_and_toxin_genes_in_E__coli_strains_isolated_from_diarrheic_and_non_diarrheic_pigs_from_smallholder_herds_in_northern_and_eastern_Uganda_ L2 - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0796-2 DB - PRIME DP - Unbound Medicine ER -