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The effect of conjugated linoleic acid supplements on oxidative and antioxidative status of dairy cows.
J Dairy Sci. 2016 Oct; 99(10):8090-8102.JD

Abstract

Dairy cows develop frequently negative energy balance around parturition and in early lactation, resulting in excessive mobilization of body fat and subsequently in increased risk of ketosis and other diseases. Dietary conjugated linoleic acid (CLA) supplements are used in dairy cows mainly for their depressing effect on milk fat content, but are also proposed to have antioxidative properties. As negative energy balance is associated with oxidative stress, which is also assumed to contribute to disease development, the present study was conducted to examine effects of CLA on oxidative and antioxidative status of lactating dairy cows. German Holstein cows (primiparous n=13, multiparous n=32) were divided into 3 dietary treatment groups receiving 100g/d of control fat supplement, containing 87% stearic acid (CON; n=14), 50g/d of control fat supplement and 50g/d of CLA supplement (CLA 50; n=15), or 100g/d of CLA supplement (CLA 100; n=16). The CLA supplement was lipid-encapsulated and contained 12% of trans-10,cis-12 CLA and cis-9,trans-11 CLA each. Supplementation took place between d1 and 182 postpartum; d 182 until 252 postpartum served as a depletion period. Blood was sampled at d -21, 1, 21, 70, 105, 140, 182, 224, and 252 relative to calving. The antioxidative status was determined using the ferric-reducing ability of plasma, α-tocopherol, α-tocopherol-to-cholesterol mass ratio, and retinol. For determination of oxidative status concentrations of hydroperoxides, thiobarbituric acid-reactive substances (TBARS), N'-formylkynurenine, and bityrosine were measured. Mixed models of fixed and random effects with repeated measures were used to evaluate period 1 (d -21 to 140) and 2 (d182-252) separately. Cows showed increased oxidative stress and lipid peroxidation during the periparturient period in terms of increased serum concentrations of hydroperoxides and TBARS, which decreased throughout lactation. During period 1, the supplemented cows had lower TBARS concentrations, which was not detectable in period 2. The other determined parameters were not affected by CLA supplementation. The obtained results show that dietary CLA supplementation in the chosen dosage, formulation, and application period had a marginal antioxidative effect in terms of lipid peroxidation in lactating dairy cows.

Authors+Show Affiliations

Clinic for Cattle, University of Veterinary Medicine, Foundation, 30173, Hannover, Germany. Electronic address: nina.hanschke@tiho-hannover.de.University of Life Sciences, 20-033, Lublin, Poland.Clinic for Cattle, University of Veterinary Medicine, Foundation, 30173, Hannover, Germany.Clinic for Cattle, University of Veterinary Medicine, Foundation, 30173, Hannover, Germany.Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 38116, Braunschweig, Germany.Institute of Biological Chemistry and Nutrition, University of Hohenheim, 70599 Stuttgart, Germany.Institute of Animal Nutrition, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 38116, Braunschweig, Germany.Clinic for Cattle, University of Veterinary Medicine, Foundation, 30173, Hannover, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27497903

Citation

Hanschke, N, et al. "The Effect of Conjugated Linoleic Acid Supplements On Oxidative and Antioxidative Status of Dairy Cows." Journal of Dairy Science, vol. 99, no. 10, 2016, pp. 8090-8102.
Hanschke N, Kankofer M, Ruda L, et al. The effect of conjugated linoleic acid supplements on oxidative and antioxidative status of dairy cows. J Dairy Sci. 2016;99(10):8090-8102.
Hanschke, N., Kankofer, M., Ruda, L., Höltershinken, M., Meyer, U., Frank, J., Dänicke, S., & Rehage, J. (2016). The effect of conjugated linoleic acid supplements on oxidative and antioxidative status of dairy cows. Journal of Dairy Science, 99(10), 8090-8102. https://doi.org/10.3168/jds.2015-10685
Hanschke N, et al. The Effect of Conjugated Linoleic Acid Supplements On Oxidative and Antioxidative Status of Dairy Cows. J Dairy Sci. 2016;99(10):8090-8102. PubMed PMID: 27497903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of conjugated linoleic acid supplements on oxidative and antioxidative status of dairy cows. AU - Hanschke,N, AU - Kankofer,M, AU - Ruda,L, AU - Höltershinken,M, AU - Meyer,U, AU - Frank,J, AU - Dänicke,S, AU - Rehage,J, Y1 - 2016/08/04/ PY - 2015/11/26/received PY - 2016/06/24/accepted PY - 2016/8/8/entrez PY - 2016/8/9/pubmed PY - 2017/5/26/medline KW - antioxidant KW - conjugated linoleic acid KW - dairy cow KW - oxidative stress SP - 8090 EP - 8102 JF - Journal of dairy science JO - J Dairy Sci VL - 99 IS - 10 N2 - Dairy cows develop frequently negative energy balance around parturition and in early lactation, resulting in excessive mobilization of body fat and subsequently in increased risk of ketosis and other diseases. Dietary conjugated linoleic acid (CLA) supplements are used in dairy cows mainly for their depressing effect on milk fat content, but are also proposed to have antioxidative properties. As negative energy balance is associated with oxidative stress, which is also assumed to contribute to disease development, the present study was conducted to examine effects of CLA on oxidative and antioxidative status of lactating dairy cows. German Holstein cows (primiparous n=13, multiparous n=32) were divided into 3 dietary treatment groups receiving 100g/d of control fat supplement, containing 87% stearic acid (CON; n=14), 50g/d of control fat supplement and 50g/d of CLA supplement (CLA 50; n=15), or 100g/d of CLA supplement (CLA 100; n=16). The CLA supplement was lipid-encapsulated and contained 12% of trans-10,cis-12 CLA and cis-9,trans-11 CLA each. Supplementation took place between d1 and 182 postpartum; d 182 until 252 postpartum served as a depletion period. Blood was sampled at d -21, 1, 21, 70, 105, 140, 182, 224, and 252 relative to calving. The antioxidative status was determined using the ferric-reducing ability of plasma, α-tocopherol, α-tocopherol-to-cholesterol mass ratio, and retinol. For determination of oxidative status concentrations of hydroperoxides, thiobarbituric acid-reactive substances (TBARS), N'-formylkynurenine, and bityrosine were measured. Mixed models of fixed and random effects with repeated measures were used to evaluate period 1 (d -21 to 140) and 2 (d182-252) separately. Cows showed increased oxidative stress and lipid peroxidation during the periparturient period in terms of increased serum concentrations of hydroperoxides and TBARS, which decreased throughout lactation. During period 1, the supplemented cows had lower TBARS concentrations, which was not detectable in period 2. The other determined parameters were not affected by CLA supplementation. The obtained results show that dietary CLA supplementation in the chosen dosage, formulation, and application period had a marginal antioxidative effect in terms of lipid peroxidation in lactating dairy cows. SN - 1525-3198 UR - https://www.unboundmedicine.com/medline/citation/27497903/The_effect_of_conjugated_linoleic_acid_supplements_on_oxidative_and_antioxidative_status_of_dairy_cows_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(16)30485-4 DB - PRIME DP - Unbound Medicine ER -