Vine nitrogen status and volatile thiols and their precursors from plot to transcriptome level.
BMC Plant Biol. 2016 08 08; 16(1):173.BP

Abstract

BACKGROUND

Volatile thiols largely contribute to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless, non-volatile precursors found in the berries and the must. The present study investigates the effects of vine nitrogen (N) status on 3SH and 4MSP content in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) in the berries and the must. This is paralleled by a RNA-seq analysis of gene expression in the berries. The impact of N supply on the expression of the glutathione-S-transferase 3 and 4 (VviGST3 and VviGST4) and the γ-glutamyltranspeptidase (VviGGT), considered as key genes in their biosynthesis, was also evaluated.

RESULTS

N supply (N100 treatment) increased the 3SH content in wine while no effect was noticed on 4MSP level. Furthermore, N supply increased Glut-3SH levels in grape berries at late berry ripening stages, and this effect was highly significant in must at harvest. No significant effect of N addition was noticed on Cys-3SH concentration. The transcript abundance of the glutathione-S-transferases VviGST3 and VviGST4 and the γ-glutamyltranspeptidase (VviGGT), were similar between the control and the N100 treatment. New candidate genes which might be implicated in the biosynthetic pathway of 3SH precursors were identified by whole transcriptome shotgun sequencing (RNA-seq).

CONCLUSIONS

High vine N status has a positive effect on 3SH content in wine through an increase of Glut-3SH levels in grape berries and must. Candidate GSTs and glutathione-S-conjugates type transporters involved in this stimulation were identified by RNA-seq analysis.

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Authors+Show Affiliations

Helwi P

Univ. de Bordeaux, Institut des Sciences de la Vigne et du Vin (ISVV), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), UMR 1287, 33140, Villenave d'Ornon, France. Bordeaux Sciences Agro, Institut des Sciences de la Vigne et du Vin (ISVV), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), UMR 1287, 33140, Villenave d'Ornon, France. INRA, Institut des Sciences de la Vigne et du Vin (ISVV), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), UMR 1287, 33140, Villenave d'Ornon, France.

Guillaumie S

Thibon C

Univ. de Bordeaux, Institut des Sciences de la Vigne et du Vin (ISVV), Unité de recherche Œnologie, EA 4577, 33140, Villenave d'Ornon, France. INRA, Institut des Sciences de la Vigne et du Vin (ISVV), USC 1366 Œnologie, 33140, Villenave d'Ornon, France.

Keime C

Univ. de Strasbourg, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IBGMC), Institut National de la Santé et de la Recherche Médicale U 964, Centre National de Recherche Scientifique UMR 7104, 67404, Illkirch, France.

Bordeaux Sciences Agro, Institut des Sciences de la Vigne et du Vin (ISVV), Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), UMR 1287, 33140, Villenave d'Ornon, France. vanleeuwen@agro-bordeaux.fr.

MeSH

FermentationFruitGlutathioneNitrogenPlant ProteinsSaccharomyces cerevisiaeSulfhydryl CompoundsTranscriptomeVitisWine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27498539

Citation

Helwi, Pierre, et al. "Vine Nitrogen Status and Volatile Thiols and Their Precursors From Plot to Transcriptome Level." BMC Plant Biology, vol. 16, no. 1, 2016, p. 173.

Helwi P, Guillaumie S, Thibon C, et al. Vine nitrogen status and volatile thiols and their precursors from plot to transcriptome level. BMC Plant Biol. 2016;16(1):173.

Helwi, P., Guillaumie, S., Thibon, C., Keime, C., Habran, A., Hilbert, G., Gomes, E., Darriet, P., Delrot, S., & van Leeuwen, C. (2016). Vine nitrogen status and volatile thiols and their precursors from plot to transcriptome level. BMC Plant Biology, 16(1), 173. https://doi.org/10.1186/s12870-016-0836-y

Helwi P, et al. Vine Nitrogen Status and Volatile Thiols and Their Precursors From Plot to Transcriptome Level. BMC Plant Biol. 2016 08 8;16(1):173. PubMed PMID: 27498539.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Vine nitrogen status and volatile thiols and their precursors from plot to transcriptome level. AU - Helwi,Pierre, AU - Guillaumie,Sabine, AU - Thibon,Cécile, AU - Keime,Céline, AU - Habran,Aude, AU - Hilbert,Ghislaine, AU - Gomes,Eric, AU - Darriet,Philippe, AU - Delrot,Serge, AU - van Leeuwen,Cornelis, Y1 - 2016/08/08/ PY - 2016/03/04/received PY - 2016/06/20/accepted PY - 2016/8/9/entrez PY - 2016/8/9/pubmed PY - 2017/8/19/medline KW - 3-sulfanylhexan-1-ol KW - 4-methyl-4-sulfanylpentan-2-one KW - Cysteinylated precursors KW - GGT KW - GST KW - Glutathionylated precursors KW - Nitrogen KW - Vitis vinifera KW - Volatile thiols SP - 173 EP - 173 JF - BMC plant biology JO - BMC Plant Biol VL - 16 IS - 1 N2 - BACKGROUND: Volatile thiols largely contribute to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless, non-volatile precursors found in the berries and the must. The present study investigates the effects of vine nitrogen (N) status on 3SH and 4MSP content in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) in the berries and the must. This is paralleled by a RNA-seq analysis of gene expression in the berries. The impact of N supply on the expression of the glutathione-S-transferase 3 and 4 (VviGST3 and VviGST4) and the γ-glutamyltranspeptidase (VviGGT), considered as key genes in their biosynthesis, was also evaluated. RESULTS: N supply (N100 treatment) increased the 3SH content in wine while no effect was noticed on 4MSP level. Furthermore, N supply increased Glut-3SH levels in grape berries at late berry ripening stages, and this effect was highly significant in must at harvest. No significant effect of N addition was noticed on Cys-3SH concentration. The transcript abundance of the glutathione-S-transferases VviGST3 and VviGST4 and the γ-glutamyltranspeptidase (VviGGT), were similar between the control and the N100 treatment. New candidate genes which might be implicated in the biosynthetic pathway of 3SH precursors were identified by whole transcriptome shotgun sequencing (RNA-seq). CONCLUSIONS: High vine N status has a positive effect on 3SH content in wine through an increase of Glut-3SH levels in grape berries and must. Candidate GSTs and glutathione-S-conjugates type transporters involved in this stimulation were identified by RNA-seq analysis. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/27498539/Vine_nitrogen_status_and_volatile_thiols_and_their_precursors_from_plot_to_transcriptome_level_ DB - PRIME DP - Unbound Medicine ER -

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Vine nitrogen status and volatile thiols and their precursors from plot to transcriptome level.BMC Plant Biol. 2016 08 08; 16(1):173.BP