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A Stenohaline Medaka, Oryzias woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress.
Zoolog Sci. 2016 Aug; 33(4):414-25.ZS

Abstract

The present study aimed to evaluate the osmoregulatory mechanism of Daisy's medaka, O. woworae,as well as demonstrate the major factors affecting the hypo-osmoregulatory characteristics of euryhaline and stenohaline medaka. The medaka phylogenetic tree indicates that Daisy's medaka belongs to the celebensis species group. The salinity tolerance of Daisy's medaka was assessed. Our findings revealed that 20‰ (hypertonic) saltwater (SW) was lethal to Daisy's medaka. However, 62.5% of individuals survived 10‰ (isotonic) SW with pre-acclimation to 5‰ SW for one week. This transfer regime, "Experimental (Exp.) 10‰ SW", was used in the following experiments. After 10‰ SW-transfer, the plasma osmolality of Daisy's medaka significantly increased. The protein abundance and distribution of branchial Na(+), K(+)-ATPase (NKA) and Na(+), K(+), 2Cl(-) cotransporter 1 (NKCC1) were also examined after transfer to 10‰ SW for one week. Gill NKA activity increased significantly after transfer to 10‰ SW. Meanwhile, elevation of gill NKA αα-subunit protein-abundance was found in the 10‰ SW-acclimated fish. In gill cross-sections, more and larger NKA-immunoreactive (NKA-IR) cells were observed in the Exp. 10‰ SW medaka. The relative abundance of branchial NKCC1 protein increased significantly after transfer to 10‰ SW. NKCC1 was distributed in the basolateral membrane of NKA-IR cells of the Exp. 10‰ SW group. Furthermore, a higher abundance of NKCC1 protein was found in the gill homogenates of the euryhaline medaka, O. dancena, than in that of the stenohaline medaka, O. woworae.

Authors+Show Affiliations

1 Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. † JJJ, CKK, and WKY contributed equally to this paper.2 Tainan Hydraulics Laboratory, National Cheng Kung University, Tainan 709, Taiwan. † JJJ, CKK, and WKY contributed equally to this paper.1 Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. † JJJ, CKK, and WKY contributed equally to this paper.1 Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan.1 Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. 3 Department of Biological Science and Technology, China Medical University,Taichung 404, Taiwan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27498801

Citation

Juo, Jiun-Jang, et al. "A Stenohaline Medaka, Oryzias Woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress." Zoological Science, vol. 33, no. 4, 2016, pp. 414-25.
Juo JJ, Kang CK, Yang WK, et al. A Stenohaline Medaka, Oryzias woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress. Zoolog Sci. 2016;33(4):414-25.
Juo, J. J., Kang, C. K., Yang, W. K., Yang, S. Y., & Lee, T. H. (2016). A Stenohaline Medaka, Oryzias woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress. Zoological Science, 33(4), 414-25. https://doi.org/10.2108/zs150157
Juo JJ, et al. A Stenohaline Medaka, Oryzias Woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress. Zoolog Sci. 2016;33(4):414-25. PubMed PMID: 27498801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Stenohaline Medaka, Oryzias woworae, Increases Expression of Gill Na(+), K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress. AU - Juo,Jiun-Jang, AU - Kang,Chao-Kai, AU - Yang,Wen-Kai, AU - Yang,Shu-Yuan, AU - Lee,Tsung-Han, PY - 2016/8/9/entrez PY - 2016/8/9/pubmed PY - 2017/1/28/medline KW - 2Cl- cotransporter 1 KW - K+ KW - K+-ATPase KW - Na+ KW - Oryzias woworae KW - gill KW - osmotic stress KW - stenohaline SP - 414 EP - 25 JF - Zoological science JO - Zoolog Sci VL - 33 IS - 4 N2 - The present study aimed to evaluate the osmoregulatory mechanism of Daisy's medaka, O. woworae,as well as demonstrate the major factors affecting the hypo-osmoregulatory characteristics of euryhaline and stenohaline medaka. The medaka phylogenetic tree indicates that Daisy's medaka belongs to the celebensis species group. The salinity tolerance of Daisy's medaka was assessed. Our findings revealed that 20‰ (hypertonic) saltwater (SW) was lethal to Daisy's medaka. However, 62.5% of individuals survived 10‰ (isotonic) SW with pre-acclimation to 5‰ SW for one week. This transfer regime, "Experimental (Exp.) 10‰ SW", was used in the following experiments. After 10‰ SW-transfer, the plasma osmolality of Daisy's medaka significantly increased. The protein abundance and distribution of branchial Na(+), K(+)-ATPase (NKA) and Na(+), K(+), 2Cl(-) cotransporter 1 (NKCC1) were also examined after transfer to 10‰ SW for one week. Gill NKA activity increased significantly after transfer to 10‰ SW. Meanwhile, elevation of gill NKA αα-subunit protein-abundance was found in the 10‰ SW-acclimated fish. In gill cross-sections, more and larger NKA-immunoreactive (NKA-IR) cells were observed in the Exp. 10‰ SW medaka. The relative abundance of branchial NKCC1 protein increased significantly after transfer to 10‰ SW. NKCC1 was distributed in the basolateral membrane of NKA-IR cells of the Exp. 10‰ SW group. Furthermore, a higher abundance of NKCC1 protein was found in the gill homogenates of the euryhaline medaka, O. dancena, than in that of the stenohaline medaka, O. woworae. SN - 0289-0003 UR - https://www.unboundmedicine.com/medline/citation/27498801/A_Stenohaline_Medaka_Oryzias_woworae_Increases_Expression_of_Gill_Na_+__K_+__ATPase_and_Na_+__K_+__2Cl____Cotransporter_1_to_Tolerate_Osmotic_Stress_ L2 - https://doi.org/10.2108/zs150157 DB - PRIME DP - Unbound Medicine ER -