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Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio).
Nanotoxicology. 2016 11; 10(9):1363-72.N

Abstract

Nanoparticles (NPs, 1-100 nm) can enter the environment and result in exposure to humans and other organisms leading to potential adverse health effects. The aim of the present study is to evaluate the effects of early life exposure to polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs, 50 nm), particularly with respect to vascular toxicity on zebrafish embryos and larvae (Danio rerio). Previously published data has suggested that PVP-AgNP exposure can inhibit the expression of genes within the vascular endothelial growth factor (VEGF) signaling pathway, leading to delayed and abnormal vascular development. Here, we show that early acute exposure (0-12 h post-fertilization, hpf) of embryos to PVP-AgNPs at 1 mg/L or higher results in a transient, dose-dependent induction in VEGF-related gene expression that returns to baseline levels at hatching (72 hpf). Hatching results in normoxia, negating the effects of AgNPs on vascular development. Interestingly, increased gene transcription was not followed by the production of associated proteins within the VEGF pathway, which we attribute to NP-induced stress in the endoplasmic reticulum (ER). The impaired translation may be responsible for the observed delays in vascular development at later stages, and for smaller larvae size at hatching. Silver ion (Ag(+)) concentrations were < 0.001 mg/L at all times, with no significant effects on the VEGF pathway. We propose that PVP-AgNPs temporarily delay embryonic vascular development by interfering with oxygen diffusion into the egg, leading to hypoxic conditions and ER stress.

Authors+Show Affiliations

a Department of Forestry and Natural Resources and Bindley Biosciences Center .a Department of Forestry and Natural Resources and Bindley Biosciences Center .b Department of Animal Sciences , and.c Department of Chemistry , Purdue University , West Lafayette, IN , USA.c Department of Chemistry , Purdue University , West Lafayette, IN , USA.a Department of Forestry and Natural Resources and Bindley Biosciences Center .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27499207

Citation

Gao, Jiejun, et al. "Vascular Toxicity of Silver Nanoparticles to Developing Zebrafish (Danio Rerio)." Nanotoxicology, vol. 10, no. 9, 2016, pp. 1363-72.
Gao J, Mahapatra CT, Mapes CD, et al. Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio). Nanotoxicology. 2016;10(9):1363-72.
Gao, J., Mahapatra, C. T., Mapes, C. D., Khlebnikova, M., Wei, A., & Sepúlveda, M. S. (2016). Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio). Nanotoxicology, 10(9), 1363-72. https://doi.org/10.1080/17435390.2016.1214763
Gao J, et al. Vascular Toxicity of Silver Nanoparticles to Developing Zebrafish (Danio Rerio). Nanotoxicology. 2016;10(9):1363-72. PubMed PMID: 27499207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vascular toxicity of silver nanoparticles to developing zebrafish (Danio rerio). AU - Gao,Jiejun, AU - Mahapatra,Cecon T, AU - Mapes,Christopher D, AU - Khlebnikova,Maria, AU - Wei,Alexander, AU - Sepúlveda,Marisol S, Y1 - 2016/08/08/ PY - 2016/8/9/entrez PY - 2016/8/9/pubmed PY - 2017/6/16/medline KW - Nanoparticles KW - VEGF pathway KW - vascular toxicity KW - zebrafish embryos SP - 1363 EP - 72 JF - Nanotoxicology JO - Nanotoxicology VL - 10 IS - 9 N2 - Nanoparticles (NPs, 1-100 nm) can enter the environment and result in exposure to humans and other organisms leading to potential adverse health effects. The aim of the present study is to evaluate the effects of early life exposure to polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs, 50 nm), particularly with respect to vascular toxicity on zebrafish embryos and larvae (Danio rerio). Previously published data has suggested that PVP-AgNP exposure can inhibit the expression of genes within the vascular endothelial growth factor (VEGF) signaling pathway, leading to delayed and abnormal vascular development. Here, we show that early acute exposure (0-12 h post-fertilization, hpf) of embryos to PVP-AgNPs at 1 mg/L or higher results in a transient, dose-dependent induction in VEGF-related gene expression that returns to baseline levels at hatching (72 hpf). Hatching results in normoxia, negating the effects of AgNPs on vascular development. Interestingly, increased gene transcription was not followed by the production of associated proteins within the VEGF pathway, which we attribute to NP-induced stress in the endoplasmic reticulum (ER). The impaired translation may be responsible for the observed delays in vascular development at later stages, and for smaller larvae size at hatching. Silver ion (Ag(+)) concentrations were < 0.001 mg/L at all times, with no significant effects on the VEGF pathway. We propose that PVP-AgNPs temporarily delay embryonic vascular development by interfering with oxygen diffusion into the egg, leading to hypoxic conditions and ER stress. SN - 1743-5404 UR - https://www.unboundmedicine.com/medline/citation/27499207/Vascular_toxicity_of_silver_nanoparticles_to_developing_zebrafish__Danio_rerio__ L2 - https://www.tandfonline.com/doi/full/10.1080/17435390.2016.1214763 DB - PRIME DP - Unbound Medicine ER -