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Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii.
Genetica. 2016 Oct; 144(5):523-533.G

Abstract

Hybrid necrosis has been observed in many interspecific hybrids from crosses between tetraploid wheat and the wheat D-genome donor Aegilops tauschii. Type II necrosis is a kind of hybrid incompatibility that is specifically characterized by low-temperature induction and growth suppression. Two complementary genes, Net1 on the AB genome and Net2 on the D genome, putatively control type II necrosis in ABD triploids and synthetic hexaploid wheat. Toward map-based cloning of Net2, a fine map around the Net2 region on 2DS was constructed in this study. Using the draft genome sequence of Ae. tauschii and the physical map of the barley genome, the Net2 locus was mapped within a 0.6 cM interval between two closely linked markers. Although local chromosomal rearrangements were observed in the Net2-corresponding region between the barley/Brachypodium and Ae. tauschii genomes, the two closely linked markers were significantly associated with type II necrosis in Ae. tauschii. These results suggest that these markers will aid efficient selection of Net2 non-carrier individuals from the Ae. tauschii population and intraspecific progeny, and could help with introgression of agriculturally important genes from Ae. tauschii to common wheat.

Authors+Show Affiliations

Sakaguchi K
Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan.
Nishijima R
Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan.
Iehisa JC
Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan.
Takumi S
Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan. takumi@kobe-u.ac.jp.

MeSH

Chromosome MappingChromosomes, PlantCrosses, GeneticGenes, PlantGenetic Association StudiesGenetic LinkageGenetic MarkersGenotypePhenotypePoaceaeTetraploidyTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27502693

Citation

Sakaguchi, Kouhei, et al. "Fine Mapping and Genetic Association Analysis of Net2, the Causative D-genome Locus of Low Temperature-induced Hybrid Necrosis in Interspecific Crosses Between Tetraploid Wheat and Aegilops Tauschii." Genetica, vol. 144, no. 5, 2016, pp. 523-533.
Sakaguchi K, Nishijima R, Iehisa JC, et al. Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii. Genetica. 2016;144(5):523-533.
Sakaguchi, K., Nishijima, R., Iehisa, J. C., & Takumi, S. (2016). Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii. Genetica, 144(5), 523-533.
Sakaguchi K, et al. Fine Mapping and Genetic Association Analysis of Net2, the Causative D-genome Locus of Low Temperature-induced Hybrid Necrosis in Interspecific Crosses Between Tetraploid Wheat and Aegilops Tauschii. Genetica. 2016;144(5):523-533. PubMed PMID: 27502693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fine mapping and genetic association analysis of Net2, the causative D-genome locus of low temperature-induced hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii. AU - Sakaguchi,Kouhei, AU - Nishijima,Ryo, AU - Iehisa,Julio Cesar Masaru, AU - Takumi,Shigeo, Y1 - 2016/08/08/ PY - 2016/05/07/received PY - 2016/08/02/accepted PY - 2016/8/10/pubmed PY - 2017/2/28/medline PY - 2016/8/10/entrez KW - Allopolyploid evolution KW - Chromosome synteny KW - Hybrid incompatibility KW - Single nucleotide polymorphism KW - Wheat SP - 523 EP - 533 JF - Genetica JO - Genetica VL - 144 IS - 5 N2 - Hybrid necrosis has been observed in many interspecific hybrids from crosses between tetraploid wheat and the wheat D-genome donor Aegilops tauschii. Type II necrosis is a kind of hybrid incompatibility that is specifically characterized by low-temperature induction and growth suppression. Two complementary genes, Net1 on the AB genome and Net2 on the D genome, putatively control type II necrosis in ABD triploids and synthetic hexaploid wheat. Toward map-based cloning of Net2, a fine map around the Net2 region on 2DS was constructed in this study. Using the draft genome sequence of Ae. tauschii and the physical map of the barley genome, the Net2 locus was mapped within a 0.6 cM interval between two closely linked markers. Although local chromosomal rearrangements were observed in the Net2-corresponding region between the barley/Brachypodium and Ae. tauschii genomes, the two closely linked markers were significantly associated with type II necrosis in Ae. tauschii. These results suggest that these markers will aid efficient selection of Net2 non-carrier individuals from the Ae. tauschii population and intraspecific progeny, and could help with introgression of agriculturally important genes from Ae. tauschii to common wheat. SN - 1573-6857 UR - https://www.unboundmedicine.com/medline/citation/27502693/Fine_mapping_and_genetic_association_analysis_of_Net2_the_causative_D_genome_locus_of_low_temperature_induced_hybrid_necrosis_in_interspecific_crosses_between_tetraploid_wheat_and_Aegilops_tauschii_ DB - PRIME DP - Unbound Medicine ER -
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