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Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism.
JPEN J Parenter Enteral Nutr. 2017 11; 41(8):1293-1300.JJ

Abstract

BACKGROUND

Exogenous glucagon-like peptide 2 (GLP-2) stimulates intestinal adaptation after resection in animal models of pediatric short bowel syndrome (SBS). It is unknown whether the molecular mechanisms of such GLP-2 effects are similar to those of postresection spontaneous adaptation. Using preterm pigs as a model, we hypothesized that GLP-2 treatment would change the intestinal proteome within the first week after resection, relative to individuals not resected or resected without GLP-2 treatment.

MATERIALS AND METHODS

Two-day-old preterm pigs were subjected to resection of 50% distal small intestine and fed total parenteral nutrition without (SBS) or with GLP-2 infusion (3.5 µg/kg/h, SBS+GLP-2) for 5 days. The proteome of the remnant proximal intestine was compared among the SBS, SBS+GLP-2, and unresected pigs, through gel-based proteomics.

RESULTS

Thirty-two proteins with differential expression were identified. Ten of these proteins were affected by the resection alone (ie, SBS vs unresected pigs). Five of these resection-responsive proteins and another 22 proteins were affected by GLP-2 infusion (ie, SBS+GLP-2 vs SBS or unresected pigs). Resection alone mainly affected cellular structural proteins, while the added GLP-2 treatment affected proteins involved in protein processing and the metabolism of protein, carbohydrate, and sulphur.

CONCLUSION

In the first days following resection, proteins affected by resection plus GLP-2 treatment differed markedly from those affected by the spontaneous intestinal adaptation following resection alone. Whether more long-term GLP-2 treatment may affect the intestinal proteome following intestinal resection remains unknown.

Authors+Show Affiliations

1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark. 2 School of Biological Sciences, The University of Hong Kong, Hong Kong, China.1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark. Global Research, Novo Nordisk, Måløv, Denmark.1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.2 School of Biological Sciences, The University of Hong Kong, Hong Kong, China.1 Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27503937

Citation

Jiang, Pingping, et al. "Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs By Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism." JPEN. Journal of Parenteral and Enteral Nutrition, vol. 41, no. 8, 2017, pp. 1293-1300.
Jiang P, Vegge A, Thymann T, et al. Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN J Parenter Enteral Nutr. 2017;41(8):1293-1300.
Jiang, P., Vegge, A., Thymann, T., Wan, J. M., & Sangild, P. T. (2017). Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN. Journal of Parenteral and Enteral Nutrition, 41(8), 1293-1300. https://doi.org/10.1177/0148607116662971
Jiang P, et al. Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs By Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. JPEN J Parenter Enteral Nutr. 2017;41(8):1293-1300. PubMed PMID: 27503937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucagon-Like Peptide 2 Stimulates Postresection Intestinal Adaptation in Preterm Pigs by Affecting Proteins Related to Protein, Carbohydrate, and Sulphur Metabolism. AU - Jiang,Pingping, AU - Vegge,Andreas, AU - Thymann,Thomas, AU - Wan,Jennifer Man-Fan, AU - Sangild,Per Torp, Y1 - 2016/08/08/ PY - 2016/8/10/pubmed PY - 2018/7/20/medline PY - 2016/8/10/entrez KW - GLP-2 KW - intestinal adaptation KW - preterm pig KW - proteomics KW - research and diseases KW - short bowel syndrome SP - 1293 EP - 1300 JF - JPEN. Journal of parenteral and enteral nutrition JO - JPEN J Parenter Enteral Nutr VL - 41 IS - 8 N2 - BACKGROUND: Exogenous glucagon-like peptide 2 (GLP-2) stimulates intestinal adaptation after resection in animal models of pediatric short bowel syndrome (SBS). It is unknown whether the molecular mechanisms of such GLP-2 effects are similar to those of postresection spontaneous adaptation. Using preterm pigs as a model, we hypothesized that GLP-2 treatment would change the intestinal proteome within the first week after resection, relative to individuals not resected or resected without GLP-2 treatment. MATERIALS AND METHODS: Two-day-old preterm pigs were subjected to resection of 50% distal small intestine and fed total parenteral nutrition without (SBS) or with GLP-2 infusion (3.5 µg/kg/h, SBS+GLP-2) for 5 days. The proteome of the remnant proximal intestine was compared among the SBS, SBS+GLP-2, and unresected pigs, through gel-based proteomics. RESULTS: Thirty-two proteins with differential expression were identified. Ten of these proteins were affected by the resection alone (ie, SBS vs unresected pigs). Five of these resection-responsive proteins and another 22 proteins were affected by GLP-2 infusion (ie, SBS+GLP-2 vs SBS or unresected pigs). Resection alone mainly affected cellular structural proteins, while the added GLP-2 treatment affected proteins involved in protein processing and the metabolism of protein, carbohydrate, and sulphur. CONCLUSION: In the first days following resection, proteins affected by resection plus GLP-2 treatment differed markedly from those affected by the spontaneous intestinal adaptation following resection alone. Whether more long-term GLP-2 treatment may affect the intestinal proteome following intestinal resection remains unknown. SN - 1941-2444 UR - https://www.unboundmedicine.com/medline/citation/27503937/Glucagon_Like_Peptide_2_Stimulates_Postresection_Intestinal_Adaptation_in_Preterm_Pigs_by_Affecting_Proteins_Related_to_Protein_Carbohydrate_and_Sulphur_Metabolism_ DB - PRIME DP - Unbound Medicine ER -