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Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus.
Indian J Pathol Microbiol. 2016 Jul-Sep; 59(3):314-7.IJ

Abstract

AIMS

Staphylococcus aureus is a common pathogen causing a wide range of infections ranging from mild skin and soft tissue infections to severe, life-threatening infections. Accuracy in the detection of methicillin resistance is important to avoid treatment failures. The aim of this study was to compare the results of phenotypic and genotypic test methods to detect methicillin resistance and also to determine the antimicrobial susceptibilities.

MATERIALS AND METHODS

Two hundred and forty-two S. aureus strains isolated from skin and soft tissue samples were analyzed for methicillin resistance using oxacillin and cefoxitin disk diffusion (DD), oxacillin screen agar test, cefoxitin E-test, and mecA gene polymerase chain reaction (PCR).

RESULTS

77 of 242 S. aureus isolates were mecA positive. Oxacillin, cefoxitin DD, oxacillin screen agar test and cefoxitin E-test exhibited sensitivities as 98.7%, 98.7%, 100%, 100%, and specificities as 96.9%, 97.5%, 96.9%, 97.5%, respectively.

CONCLUSION

Results of oxacillin screen agar and cefoxitin DD test were in concordance with mecA gene PCR. Thus, it is determined that especially cefoxitin test can be an alternative to PCR in routine.

Authors+Show Affiliations

Department of Clinical Microbiology, Ahi Evran University Research and Training Hospital, Kirsehir; Department of Communicable Diseases Control Programs, Public Health Institution, National Microbiology Reference Laboratories, Ankara, Turkey.Department of Clinical Microbiology, Ministry of Health, Dıskapı Research and Training Hospital, Ankara, Turkey.Department of Clinical Microbiology, Ankara Research and Training Hospital, Ankara, Turkey.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

27510667

Citation

Demir, Tulin, et al. "Comparative Analysis of Phenotypic and Genotypic Detection of Methicillin Resistance Among Staphylococcus Aureus." Indian Journal of Pathology & Microbiology, vol. 59, no. 3, 2016, pp. 314-7.
Demir T, Coplu N, Esen B. Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus. Indian J Pathol Microbiol. 2016;59(3):314-7.
Demir, T., Coplu, N., & Esen, B. (2016). Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus. Indian Journal of Pathology & Microbiology, 59(3), 314-7. https://doi.org/10.4103/0377-4929.188103
Demir T, Coplu N, Esen B. Comparative Analysis of Phenotypic and Genotypic Detection of Methicillin Resistance Among Staphylococcus Aureus. Indian J Pathol Microbiol. 2016;59(3):314-7. PubMed PMID: 27510667.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparative analysis of phenotypic and genotypic detection of methicillin resistance among Staphylococcus aureus. AU - Demir,Tulin, AU - Coplu,Nilay, AU - Esen,Berrin, PY - 2016/8/12/entrez PY - 2016/8/12/pubmed PY - 2017/3/14/medline SP - 314 EP - 7 JF - Indian journal of pathology & microbiology JO - Indian J Pathol Microbiol VL - 59 IS - 3 N2 - AIMS: Staphylococcus aureus is a common pathogen causing a wide range of infections ranging from mild skin and soft tissue infections to severe, life-threatening infections. Accuracy in the detection of methicillin resistance is important to avoid treatment failures. The aim of this study was to compare the results of phenotypic and genotypic test methods to detect methicillin resistance and also to determine the antimicrobial susceptibilities. MATERIALS AND METHODS: Two hundred and forty-two S. aureus strains isolated from skin and soft tissue samples were analyzed for methicillin resistance using oxacillin and cefoxitin disk diffusion (DD), oxacillin screen agar test, cefoxitin E-test, and mecA gene polymerase chain reaction (PCR). RESULTS: 77 of 242 S. aureus isolates were mecA positive. Oxacillin, cefoxitin DD, oxacillin screen agar test and cefoxitin E-test exhibited sensitivities as 98.7%, 98.7%, 100%, 100%, and specificities as 96.9%, 97.5%, 96.9%, 97.5%, respectively. CONCLUSION: Results of oxacillin screen agar and cefoxitin DD test were in concordance with mecA gene PCR. Thus, it is determined that especially cefoxitin test can be an alternative to PCR in routine. SN - 0974-5130 UR - https://www.unboundmedicine.com/medline/citation/27510667/Comparative_analysis_of_phenotypic_and_genotypic_detection_of_methicillin_resistance_among_Staphylococcus_aureus_ L2 - http://www.ijpmonline.org/article.asp?issn=0377-4929;year=2016;volume=59;issue=3;spage=314;epage=317;aulast=Demir DB - PRIME DP - Unbound Medicine ER -