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Dissecting the polysaccharide-rich grape cell wall matrix using recombinant pectinases during winemaking.
Carbohydr Polym. 2016 Nov 05; 152:510-519.CP

Abstract

The effectiveness of enzyme-mediated-maceration in red winemaking relies on the use of an optimum combination of specific enzymes. A lack of information on the relevant enzyme activities and the corresponding polysaccharide-rich berry cell wall structure is a major limitation. This study used different combinations of purified recombinant pectinases with cell wall profiling tools to follow the deconstruction process during winemaking. Multivariate data analysis of the glycan microarray (CoMPP) and gas chromatography (GC) results revealed that pectin lyase performed almost as effectively in de-pectination as certain commercial enzyme mixtures. Surprisingly the combination of endo-polygalacturonase and pectin-methyl-esterase only unraveled the cell walls without de-pectination. Datasets from the various combinations used confirmed pectin-rich and xyloglucan-rich layers within the grape pomace. These data support a proposed grape cell wall model which can serve as a foundation to evaluate testable hypotheses in future studies aimed at developing tailor-made enzymes for winemaking scenarios.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Gao Y
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South Africa.
Fangel JU
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, DK-1001, Denmark.
Willats WGT
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, DK-1001, Denmark.
Vivier MA
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South Africa.
Moore JP
Institute for Wine Biotechnology, Department of Viticulture and Oenology, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South Africa. Electronic address: moorejp@sun.ac.za.

MeSH

Cell WallFruitPolygalacturonasePolysaccharidesRecombinant ProteinsVitisWine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27516299

Citation

Gao, Yu, et al. "Dissecting the Polysaccharide-rich Grape Cell Wall Matrix Using Recombinant Pectinases During Winemaking." Carbohydrate Polymers, vol. 152, 2016, pp. 510-519.
Gao Y, Fangel JU, Willats WGT, et al. Dissecting the polysaccharide-rich grape cell wall matrix using recombinant pectinases during winemaking. Carbohydr Polym. 2016;152:510-519.
Gao, Y., Fangel, J. U., Willats, W. G. T., Vivier, M. A., & Moore, J. P. (2016). Dissecting the polysaccharide-rich grape cell wall matrix using recombinant pectinases during winemaking. Carbohydrate Polymers, 152, 510-519. https://doi.org/10.1016/j.carbpol.2016.05.115
Gao Y, et al. Dissecting the Polysaccharide-rich Grape Cell Wall Matrix Using Recombinant Pectinases During Winemaking. Carbohydr Polym. 2016 Nov 5;152:510-519. PubMed PMID: 27516299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dissecting the polysaccharide-rich grape cell wall matrix using recombinant pectinases during winemaking. AU - Gao,Yu, AU - Fangel,Jonatan U, AU - Willats,William G T, AU - Vivier,Melané A, AU - Moore,John P, Y1 - 2016/06/13/ PY - 2016/04/11/received PY - 2016/05/29/revised PY - 2016/05/31/accepted PY - 2016/8/13/entrez PY - 2016/8/16/pubmed PY - 2018/1/19/medline KW - Cell wall model KW - Grape pomace KW - Multivariate data analysis KW - Pectin KW - Recombinant pectinases KW - Winemaking SP - 510 EP - 519 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 152 N2 - The effectiveness of enzyme-mediated-maceration in red winemaking relies on the use of an optimum combination of specific enzymes. A lack of information on the relevant enzyme activities and the corresponding polysaccharide-rich berry cell wall structure is a major limitation. This study used different combinations of purified recombinant pectinases with cell wall profiling tools to follow the deconstruction process during winemaking. Multivariate data analysis of the glycan microarray (CoMPP) and gas chromatography (GC) results revealed that pectin lyase performed almost as effectively in de-pectination as certain commercial enzyme mixtures. Surprisingly the combination of endo-polygalacturonase and pectin-methyl-esterase only unraveled the cell walls without de-pectination. Datasets from the various combinations used confirmed pectin-rich and xyloglucan-rich layers within the grape pomace. These data support a proposed grape cell wall model which can serve as a foundation to evaluate testable hypotheses in future studies aimed at developing tailor-made enzymes for winemaking scenarios. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/27516299/Dissecting_the_polysaccharide_rich_grape_cell_wall_matrix_using_recombinant_pectinases_during_winemaking_ DB - PRIME DP - Unbound Medicine ER -