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Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities.
Carbohydr Polym. 2016 Nov 05; 152:605-614.CP

Abstract

The present study investigates biological activities of sulfated polysaccharides (SPs) isolated from smooth hound by precipitation with cetylpyridinium chloride (SP1) or ethanol (SP2). SP1 showed the highest amounts of sulfated groups (10.2%) and proteins (7.84%) and high molecular weight sugars. Infrared spectroscopic analysis showed typical peaks of sulfated polysaccharides, particularly for the SP1 that was characterized by the presence of O=S=O groups and acetyl groups. Interestingly, SPs displayed important angiotensin I converting enzyme (ACE) inhibitory (IC50=1.04 and 0.75mg/ml for SP1 and SP2, respectively), antibacterial (Gram+ and Gram-) and antioxidant activities (reducing power, metal chelating activity, β-carotene bleaching inhibition and DNA nicking assay). Moreover, SPs fractionation by DEAE-cellulose column chromatography showed one peak during the buffer elution phase and three major fractions during the linear gradient of NaCl. The overall data suggested that SPs could be used as natural antioxidant, antimicrobial and anti-ACE ingredient to formulate functional foods.

Authors+Show Affiliations

Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia. Electronic address: abd.ola1502@gmail.com.Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27516310

Citation

Abdelhedi, Ola, et al. "Sulfated Polysaccharides From Common Smooth Hound: Extraction and Assessment of anti-ACE, Antioxidant and Antibacterial Activities." Carbohydrate Polymers, vol. 152, 2016, pp. 605-614.
Abdelhedi O, Nasri R, Souissi N, et al. Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities. Carbohydr Polym. 2016;152:605-614.
Abdelhedi, O., Nasri, R., Souissi, N., Nasri, M., & Jridi, M. (2016). Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities. Carbohydrate Polymers, 152, 605-614. https://doi.org/10.1016/j.carbpol.2016.07.048
Abdelhedi O, et al. Sulfated Polysaccharides From Common Smooth Hound: Extraction and Assessment of anti-ACE, Antioxidant and Antibacterial Activities. Carbohydr Polym. 2016 Nov 5;152:605-614. PubMed PMID: 27516310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sulfated polysaccharides from common smooth hound: Extraction and assessment of anti-ACE, antioxidant and antibacterial activities. AU - Abdelhedi,Ola, AU - Nasri,Rim, AU - Souissi,Nabil, AU - Nasri,Moncef, AU - Jridi,Mourad, Y1 - 2016/07/15/ PY - 2016/05/24/received PY - 2016/07/12/revised PY - 2016/07/13/accepted PY - 2016/8/13/entrez PY - 2016/8/16/pubmed PY - 2018/1/19/medline KW - Anti-ACE activity KW - Antioxidant and antibacterial activities KW - Infrared spectroscopic analysis KW - Molecular weight distribution KW - Purification and monosaccharide composition KW - Smooth hound sulfated polysaccharides SP - 605 EP - 614 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 152 N2 - The present study investigates biological activities of sulfated polysaccharides (SPs) isolated from smooth hound by precipitation with cetylpyridinium chloride (SP1) or ethanol (SP2). SP1 showed the highest amounts of sulfated groups (10.2%) and proteins (7.84%) and high molecular weight sugars. Infrared spectroscopic analysis showed typical peaks of sulfated polysaccharides, particularly for the SP1 that was characterized by the presence of O=S=O groups and acetyl groups. Interestingly, SPs displayed important angiotensin I converting enzyme (ACE) inhibitory (IC50=1.04 and 0.75mg/ml for SP1 and SP2, respectively), antibacterial (Gram+ and Gram-) and antioxidant activities (reducing power, metal chelating activity, β-carotene bleaching inhibition and DNA nicking assay). Moreover, SPs fractionation by DEAE-cellulose column chromatography showed one peak during the buffer elution phase and three major fractions during the linear gradient of NaCl. The overall data suggested that SPs could be used as natural antioxidant, antimicrobial and anti-ACE ingredient to formulate functional foods. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/27516310/Sulfated_polysaccharides_from_common_smooth_hound:_Extraction_and_assessment_of_anti_ACE_antioxidant_and_antibacterial_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0144-8617(16)30837-2 DB - PRIME DP - Unbound Medicine ER -