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Expression induction of P450 genes by imidacloprid in Nilaparvata lugens: A genome-scale analysis.
Pestic Biochem Physiol. 2016 Sep; 132:59-64.PB

Abstract

The overexpression of P450 monooxygenase genes is a main mechanism for the resistance to imidacloprid, a representative neonicotinoid insecticide, in Nilaparvata lugens (brown planthopper, BPH). However, only two P450 genes (CYP6AY1 and CYP6ER1), among fifty-four P450 genes identified from BPH genome database, have been reported to play important roles in imidacloprid resistance until now. In this study, after the confirmation of important roles of P450s in imidacloprid resistance by the synergism analysis, the expression induction by imidacloprid was determined for all P450 genes. In the susceptible (Sus) strain, eight P450 genes in Clade4, eight in Clade3 and two in Clade2 were up-regulated by imidacloprid, among which three genes (CYP6CS1, CYP6CW1 and CYP6ER1, all in Clade3) were increased to above 4.0-fold and eight genes to above 2.0-fold. In contrast, no P450 genes were induced in Mito clade. Eight genes induced to above 2.0-fold were selected to determine their expression and induced levels in Huzhou population, in which piperonyl butoxide showed the biggest effects on imidacloprid toxicity among eight field populations. The expression levels of seven P450 genes were higher in Huzhou population than that in Sus strain, with the biggest differences for CYP6CS1 (9.8-fold), CYP6ER1 (7.7-fold) and CYP6AY1 (5.1-fold). The induction levels for all tested genes were bigger in Sus strain than that in Huzhou population except CYP425B1. Screening the induction of P450 genes by imidacloprid in the genome-scale will provide an overall view on the possible metabolic factors in the resistance to neonicotinoid insecticides. The further work, such as the functional study of recombinant proteins, will be performed to validate the roles of these P450s in imidacloprid resistance.

Authors+Show Affiliations

Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: liuzewen@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27521914

Citation

Zhang, Jianhua, et al. "Expression Induction of P450 Genes By Imidacloprid in Nilaparvata Lugens: a Genome-scale Analysis." Pesticide Biochemistry and Physiology, vol. 132, 2016, pp. 59-64.
Zhang J, Zhang Y, Wang Y, et al. Expression induction of P450 genes by imidacloprid in Nilaparvata lugens: A genome-scale analysis. Pestic Biochem Physiol. 2016;132:59-64.
Zhang, J., Zhang, Y., Wang, Y., Yang, Y., Cang, X., & Liu, Z. (2016). Expression induction of P450 genes by imidacloprid in Nilaparvata lugens: A genome-scale analysis. Pesticide Biochemistry and Physiology, 132, 59-64. https://doi.org/10.1016/j.pestbp.2015.10.016
Zhang J, et al. Expression Induction of P450 Genes By Imidacloprid in Nilaparvata Lugens: a Genome-scale Analysis. Pestic Biochem Physiol. 2016;132:59-64. PubMed PMID: 27521914.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression induction of P450 genes by imidacloprid in Nilaparvata lugens: A genome-scale analysis. AU - Zhang,Jianhua, AU - Zhang,Yixi, AU - Wang,Yunchao, AU - Yang,Yuanxue, AU - Cang,Xinzhu, AU - Liu,Zewen, Y1 - 2015/10/21/ PY - 2015/07/18/received PY - 2015/09/25/revised PY - 2015/10/19/accepted PY - 2016/8/14/entrez PY - 2016/8/16/pubmed PY - 2017/3/16/medline KW - Expression induction KW - Imidacloprid KW - Insecticide resistance KW - Nilaparvata lugens KW - P450 genes SP - 59 EP - 64 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 132 N2 - The overexpression of P450 monooxygenase genes is a main mechanism for the resistance to imidacloprid, a representative neonicotinoid insecticide, in Nilaparvata lugens (brown planthopper, BPH). However, only two P450 genes (CYP6AY1 and CYP6ER1), among fifty-four P450 genes identified from BPH genome database, have been reported to play important roles in imidacloprid resistance until now. In this study, after the confirmation of important roles of P450s in imidacloprid resistance by the synergism analysis, the expression induction by imidacloprid was determined for all P450 genes. In the susceptible (Sus) strain, eight P450 genes in Clade4, eight in Clade3 and two in Clade2 were up-regulated by imidacloprid, among which three genes (CYP6CS1, CYP6CW1 and CYP6ER1, all in Clade3) were increased to above 4.0-fold and eight genes to above 2.0-fold. In contrast, no P450 genes were induced in Mito clade. Eight genes induced to above 2.0-fold were selected to determine their expression and induced levels in Huzhou population, in which piperonyl butoxide showed the biggest effects on imidacloprid toxicity among eight field populations. The expression levels of seven P450 genes were higher in Huzhou population than that in Sus strain, with the biggest differences for CYP6CS1 (9.8-fold), CYP6ER1 (7.7-fold) and CYP6AY1 (5.1-fold). The induction levels for all tested genes were bigger in Sus strain than that in Huzhou population except CYP425B1. Screening the induction of P450 genes by imidacloprid in the genome-scale will provide an overall view on the possible metabolic factors in the resistance to neonicotinoid insecticides. The further work, such as the functional study of recombinant proteins, will be performed to validate the roles of these P450s in imidacloprid resistance. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/27521914/Expression_induction_of_P450_genes_by_imidacloprid_in_Nilaparvata_lugens:_A_genome_scale_analysis_ DB - PRIME DP - Unbound Medicine ER -