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Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively.
Biosens Bioelectron. 2017 Jan 15; 87:101-107.BB

Abstract

A viable and simple method for preparing porous graphene network using silver nanoparticles (AgNPs) etching was proposed, and a sensitive biosensor was constructed based on the porous graphene (PGN) and horseradish peroxidase (HRP) to measure the release of H2O2 from living cells. Owing to the large surface area and versatile porous structure, the use of nanoporous materials can significantly improve the analysis performance of the biosensor by loading large amounts of enzyme and accelerating diffusion rate. Meanwhile, the constructed electrode exhibited excellent electrochemical performance toward H2O2 with a determination limit as low as 0.0267nM and wide linear range of 7 orders of magnitude, which was superior to other H2O2 electrochemical sensors. Thus, this novel biosensor can detect the H2O2 release from living cells not only under normal physiological conditions (10-8-10-7M) but also in emergency state with the increased concentration (~10-4M). This work provides tremendous potential for real-time tracking of the secretion of H2O2 in different types of physiological and pathological investigations.

Authors+Show Affiliations

Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: liuxh@nwnu.edu.cn.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: luxq@nwnu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27522483

Citation

Liu, Yidan, et al. "Horseradish Peroxidase Supported On Porous Graphene as a Novel Sensing Platform for Detection of Hydrogen Peroxide in Living Cells Sensitively." Biosensors & Bioelectronics, vol. 87, 2017, pp. 101-107.
Liu Y, Liu X, Guo Z, et al. Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively. Biosens Bioelectron. 2017;87:101-107.
Liu, Y., Liu, X., Guo, Z., Hu, Z., Xue, Z., & Lu, X. (2017). Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively. Biosensors & Bioelectronics, 87, 101-107. https://doi.org/10.1016/j.bios.2016.08.015
Liu Y, et al. Horseradish Peroxidase Supported On Porous Graphene as a Novel Sensing Platform for Detection of Hydrogen Peroxide in Living Cells Sensitively. Biosens Bioelectron. 2017 Jan 15;87:101-107. PubMed PMID: 27522483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Horseradish peroxidase supported on porous graphene as a novel sensing platform for detection of hydrogen peroxide in living cells sensitively. AU - Liu,Yidan, AU - Liu,Xiuhui, AU - Guo,Zhipan, AU - Hu,Zhongai, AU - Xue,Zhonghua, AU - Lu,Xiaoquan, Y1 - 2016/08/05/ PY - 2016/06/10/received PY - 2016/07/23/revised PY - 2016/08/05/accepted PY - 2016/8/16/pubmed PY - 2017/2/16/medline PY - 2016/8/15/entrez KW - Cell tracking KW - Electrochemical biosensor KW - Horseradish peroxidase KW - Hydrogen peroxide detection KW - Porous graphene SP - 101 EP - 107 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 87 N2 - A viable and simple method for preparing porous graphene network using silver nanoparticles (AgNPs) etching was proposed, and a sensitive biosensor was constructed based on the porous graphene (PGN) and horseradish peroxidase (HRP) to measure the release of H2O2 from living cells. Owing to the large surface area and versatile porous structure, the use of nanoporous materials can significantly improve the analysis performance of the biosensor by loading large amounts of enzyme and accelerating diffusion rate. Meanwhile, the constructed electrode exhibited excellent electrochemical performance toward H2O2 with a determination limit as low as 0.0267nM and wide linear range of 7 orders of magnitude, which was superior to other H2O2 electrochemical sensors. Thus, this novel biosensor can detect the H2O2 release from living cells not only under normal physiological conditions (10-8-10-7M) but also in emergency state with the increased concentration (~10-4M). This work provides tremendous potential for real-time tracking of the secretion of H2O2 in different types of physiological and pathological investigations. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/27522483/Horseradish_peroxidase_supported_on_porous_graphene_as_a_novel_sensing_platform_for_detection_of_hydrogen_peroxide_in_living_cells_sensitively_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(16)30774-6 DB - PRIME DP - Unbound Medicine ER -