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Properties of silver nanoparticles influencing their uptake in and toxicity to the earthworm Lumbricus rubellus following exposure in soil.
Environ Pollut. 2016 Nov; 218:870-878.EP

Abstract

Physicochemical properties of nanoparticles influence their environmental fate and toxicity, and studies investigating this are vital for a holistic approach towards a comprehensive and adequate environmental risk assessment. In this study, we investigated the effects of size, surface coating (charge) of silver nanoparticles (AgNPs) - a most commonly-used nanoparticle-type, on the bioaccumulation in, and toxicity (survival, growth, cocoon production) to the earthworm Lumbricus rubellus. AgNPs were synthesized in three sizes: 20, 35 and 50 nm. Surface-coating with bovine serum albumin (AgNP_BSA), chitosan (AgNP_Chit), or polyvinylpyrrolidone (AgNP_PVP) produced negative, positive and neutral particles respectively. In a 28-day sub-chronic reproduction toxicity test, earthworms were exposed to these AgNPs in soil (0-250 mg Ag/kg soil DW). Earthworms were also exposed to AgNO3 at concentrations below known EC50. Total Ag tissue concentration indicated uptake by earthworms was generally highest for the AgNP_BSA especially at the lower exposure concentration ranges, and seems to reach a plateau level between 50 and 100 mg Ag/kg soil DW. Reproduction was impaired at high concentrations of all AgNPs tested, with AgNP_BSA particles being the most toxic. The EC50 for the 20 nm AgNP_BSA was 66.8 mg Ag/kg soil, with exposure to <60 mg Ag/kg soil already showing a decrease in the cocoon production. Thus, based on reproductive toxicity, the particles ranked: AgNP_BSA (negative) > AgNP_PVP (neutral) > Chitosan (positive). Size had an influence on uptake and toxicity of the AgNP_PVP, but not for AgNP_BSA nor AgNP_Chit. This study provides essential information on the role of physicochemical properties of AgNPs in influencing uptake by a terrestrial organism L. rubellus under environmentally relevant conditions. It also provides evidence of the influence of surface coating (charge) and the limited effect of size in the range of 20-50 nm, in driving uptake and toxicity of the AgNPs tested.

Authors+Show Affiliations

Division of Toxicology, Wageningen University and Research Centre, Tuinlaan 5, 6703 HE, Wageningen, The Netherlands; Alterra, Wageningen University and Research Centre, Droevendaalsesteeg 3, 6708 PB, Wageningen, The Netherlands. Electronic address: drsmakama@gmail.com.Institut Català de Nanociència i Nanotecnologia (ICN2), Campus de la Universitat Autònoma de Barcelona (Campus UAB), 08193, Bellaterra, Barcelona, Spain.RIKILT- Inst. of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB, Wageningen, The Netherlands.Alterra, Wageningen University and Research Centre, Droevendaalsesteeg 3, 6708 PB, Wageningen, The Netherlands.RIKILT- Inst. of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708 WB, Wageningen, The Netherlands.Institut Català de Nanociència i Nanotecnologia (ICN2), Campus de la Universitat Autònoma de Barcelona (Campus UAB), 08193, Bellaterra, Barcelona, Spain; Vall d'Hebron Institut de Recerca (VHIR), Edificio Mediterránea, Hospital Vall d'Hebron, Passeig de la Vall d'Hebron, 119-129, 08035, Barcelona, Spain; Institut Català de Recerca i Estudis Avançats (ICREA), Passeig Lluís Companys, 23, 08010, Barcelona, Spain.Division of Toxicology, Wageningen University and Research Centre, Tuinlaan 5, 6703 HE, Wageningen, The Netherlands. Electronic address: nico.vandenbrink@wur.nl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27524251

Citation

Makama, Sunday, et al. "Properties of Silver Nanoparticles Influencing Their Uptake in and Toxicity to the Earthworm Lumbricus Rubellus Following Exposure in Soil." Environmental Pollution (Barking, Essex : 1987), vol. 218, 2016, pp. 870-878.
Makama S, Piella J, Undas A, et al. Properties of silver nanoparticles influencing their uptake in and toxicity to the earthworm Lumbricus rubellus following exposure in soil. Environ Pollut. 2016;218:870-878.
Makama, S., Piella, J., Undas, A., Dimmers, W. J., Peters, R., Puntes, V. F., & van den Brink, N. W. (2016). Properties of silver nanoparticles influencing their uptake in and toxicity to the earthworm Lumbricus rubellus following exposure in soil. Environmental Pollution (Barking, Essex : 1987), 218, 870-878. https://doi.org/10.1016/j.envpol.2016.08.016
Makama S, et al. Properties of Silver Nanoparticles Influencing Their Uptake in and Toxicity to the Earthworm Lumbricus Rubellus Following Exposure in Soil. Environ Pollut. 2016;218:870-878. PubMed PMID: 27524251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Properties of silver nanoparticles influencing their uptake in and toxicity to the earthworm Lumbricus rubellus following exposure in soil. AU - Makama,Sunday, AU - Piella,Jordi, AU - Undas,Anna, AU - Dimmers,Wim J, AU - Peters,Ruud, AU - Puntes,Victor F, AU - van den Brink,Nico W, Y1 - 2016/08/11/ PY - 2016/05/17/received PY - 2016/07/25/revised PY - 2016/08/04/accepted PY - 2016/8/16/pubmed PY - 2017/1/14/medline PY - 2016/8/16/entrez KW - Cocoon production KW - Particle characterization KW - Soil organism KW - Surface coating KW - Toxicodynamics KW - Toxicokinetics SP - 870 EP - 878 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ Pollut VL - 218 N2 - Physicochemical properties of nanoparticles influence their environmental fate and toxicity, and studies investigating this are vital for a holistic approach towards a comprehensive and adequate environmental risk assessment. In this study, we investigated the effects of size, surface coating (charge) of silver nanoparticles (AgNPs) - a most commonly-used nanoparticle-type, on the bioaccumulation in, and toxicity (survival, growth, cocoon production) to the earthworm Lumbricus rubellus. AgNPs were synthesized in three sizes: 20, 35 and 50 nm. Surface-coating with bovine serum albumin (AgNP_BSA), chitosan (AgNP_Chit), or polyvinylpyrrolidone (AgNP_PVP) produced negative, positive and neutral particles respectively. In a 28-day sub-chronic reproduction toxicity test, earthworms were exposed to these AgNPs in soil (0-250 mg Ag/kg soil DW). Earthworms were also exposed to AgNO3 at concentrations below known EC50. Total Ag tissue concentration indicated uptake by earthworms was generally highest for the AgNP_BSA especially at the lower exposure concentration ranges, and seems to reach a plateau level between 50 and 100 mg Ag/kg soil DW. Reproduction was impaired at high concentrations of all AgNPs tested, with AgNP_BSA particles being the most toxic. The EC50 for the 20 nm AgNP_BSA was 66.8 mg Ag/kg soil, with exposure to <60 mg Ag/kg soil already showing a decrease in the cocoon production. Thus, based on reproductive toxicity, the particles ranked: AgNP_BSA (negative) > AgNP_PVP (neutral) > Chitosan (positive). Size had an influence on uptake and toxicity of the AgNP_PVP, but not for AgNP_BSA nor AgNP_Chit. This study provides essential information on the role of physicochemical properties of AgNPs in influencing uptake by a terrestrial organism L. rubellus under environmentally relevant conditions. It also provides evidence of the influence of surface coating (charge) and the limited effect of size in the range of 20-50 nm, in driving uptake and toxicity of the AgNPs tested. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/27524251/Properties_of_silver_nanoparticles_influencing_their_uptake_in_and_toxicity_to_the_earthworm_Lumbricus_rubellus_following_exposure_in_soil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(16)30711-4 DB - PRIME DP - Unbound Medicine ER -