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Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants.
Int J Mol Sci. 2016 Aug 12; 17(8)IJ

Abstract

The Hessian fly, Mayetiola destructor, is a destructive pest of wheat worldwide and mainly controlled by deploying resistant cultivars. In this study, we investigated the genes that were expressed differentially between larvae in resistant plants and those in susceptible plants through RNA sequencing on the Illumina platform. Informative genes were 11,832, 14,861, 15,708, and 15,071 for the comparisons between larvae in resistant versus susceptible plants for 0.5, 1, 3, and 5 days, respectively, after larvae had reached the feeding site. The transcript abundance corresponding to 5401, 6902, 8457, and 5202 of the informative genes exhibited significant differences (p ≤ 0.05) in the respective paired comparisons. Overall, genes involved in nutrient metabolism, RNA and protein synthesis exhibited lower transcript abundance in larvae from resistant plants, indicating that resistant plants inhibited nutrient metabolism and protein production in larvae. Interestingly, the numbers of cytochrome P450 genes with higher transcript abundance in larvae from resistant plants were comparable to, or higher than those with lower transcript abundance, indicating that toxic chemicals from resistant plants may have played important roles in Hessian fly larval death. Our study also identified several families of genes encoding secreted salivary gland proteins (SSGPs) that were expressed at early stage of 1(st) instar larvae and with more genes with higher transcript abundance in larvae from resistant plants. Those SSGPs are candidate effectors with important roles in plant manipulation.

Authors+Show Affiliations

Hard Winter Wheat Genetics Research Unit, USDA-ARS, 4008 Throckmorton, Kansas State University, Manhattan, KS 66506, USA. mchen@ksu.edu. Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. mchen@ksu.edu.Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA. liu3zhen@ksu.edu.Department of Statistics, Kansas State University, Manhattan, KS 66506, USA. hwang@ksu.edu.Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. xycheng@ksu.edu.International Center for Agricultural Research in the Dry Area, Rabat 10106, Morocco. M.BOHSSINI@cgiar.org.Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. jwhitwor@k-state.edu.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27529231

Citation

Chen, Ming-Shun, et al. "Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants." International Journal of Molecular Sciences, vol. 17, no. 8, 2016.
Chen MS, Liu S, Wang H, et al. Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants. Int J Mol Sci. 2016;17(8).
Chen, M. S., Liu, S., Wang, H., Cheng, X., El Bouhssini, M., & Whitworth, R. J. (2016). Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants. International Journal of Molecular Sciences, 17(8). https://doi.org/10.3390/ijms17081324
Chen MS, et al. Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants. Int J Mol Sci. 2016 Aug 12;17(8) PubMed PMID: 27529231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants. AU - Chen,Ming-Shun, AU - Liu,Sanzhen, AU - Wang,Haiyan, AU - Cheng,Xiaoyan, AU - El Bouhssini,Mustapha, AU - Whitworth,R Jeff, Y1 - 2016/08/12/ PY - 2016/06/17/received PY - 2016/07/27/revised PY - 2016/08/05/accepted PY - 2016/8/17/entrez PY - 2016/8/17/pubmed PY - 2017/4/18/medline KW - RNA-sequencing KW - compatible interaction KW - cytochrome P450 KW - effectors KW - gall midge KW - incompatible interaction KW - plant resistance KW - salivary glands JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 8 N2 - The Hessian fly, Mayetiola destructor, is a destructive pest of wheat worldwide and mainly controlled by deploying resistant cultivars. In this study, we investigated the genes that were expressed differentially between larvae in resistant plants and those in susceptible plants through RNA sequencing on the Illumina platform. Informative genes were 11,832, 14,861, 15,708, and 15,071 for the comparisons between larvae in resistant versus susceptible plants for 0.5, 1, 3, and 5 days, respectively, after larvae had reached the feeding site. The transcript abundance corresponding to 5401, 6902, 8457, and 5202 of the informative genes exhibited significant differences (p ≤ 0.05) in the respective paired comparisons. Overall, genes involved in nutrient metabolism, RNA and protein synthesis exhibited lower transcript abundance in larvae from resistant plants, indicating that resistant plants inhibited nutrient metabolism and protein production in larvae. Interestingly, the numbers of cytochrome P450 genes with higher transcript abundance in larvae from resistant plants were comparable to, or higher than those with lower transcript abundance, indicating that toxic chemicals from resistant plants may have played important roles in Hessian fly larval death. Our study also identified several families of genes encoding secreted salivary gland proteins (SSGPs) that were expressed at early stage of 1(st) instar larvae and with more genes with higher transcript abundance in larvae from resistant plants. Those SSGPs are candidate effectors with important roles in plant manipulation. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27529231/Genes_Expressed_Differentially_in_Hessian_Fly_Larvae_Feeding_in_Resistant_and_Susceptible_Plants_ L2 - https://www.mdpi.com/resolver?pii=ijms17081324 DB - PRIME DP - Unbound Medicine ER -