Tags

Type your tag names separated by a space and hit enter

Large multiresidue analysis of pesticides in edible vegetable oils by using efficient solid-phase extraction sorbents based on quick, easy, cheap, effective, rugged and safe methodology followed by gas chromatography-tandem mass spectrometry.
J Chromatogr A. 2016 Sep 09; 1463:20-31.JC

Abstract

The aim of this research was to adapt the QuEChERS method for routine pesticide multiresidue analysis in edible vegetable oil samples using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). Several clean-up approaches were tested: (a) D-SPE with Enhanced Matrix Removal-Lipid (EMR-Lipid™); (b) D-SPE with PSA; (c) D-SPE with Z-Sep; (d) SPE with Z-Sep. Clean-up methods were evaluated in terms of fat removal from the extracts, recoveries and extraction precision for 213 pesticides in different matrices (soybean, sunflower and extra-virgin olive oil). The QuEChERS protocol with EMR-Lipid d-SPE provided the best reduction of co-extracted matrix compounds with the highest number of pesticides exhibiting mean recoveries in the 70-120% range, and the lowest relative standard deviations values (4% on average). A simple and rapid (only 5min) freeze-out step with dry ice (CO2 at -76°C) prior to d-SPE clean-up ensured much better removal of co-extracted matrix compounds in compliance of the necessity in routine analysis. Procedural Standard Calibration was established in order to compensate for recovery losses of certain pesticides and possible matrix effects. Limits of quantification were 10μgkg(-1) for the majority of the pesticides. The modified methodology was applied for the analysis of different 17 oil samples. Fourteen pesticides were detected with values lower than MRLs and their concentration ranged between 10.2 and 156.0μgkg(-1).

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Parrilla Vázquez P
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Hakme E
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Uclés S
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Cutillas V
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Martínez Galera M
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Mughari AR
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain.
Fernández-Alba AR
Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, Department of Chemistry and Physics, University of Almeria, La Cañada de San Urbano, 04120 Almería, Spain. Electronic address: amadeo@ual.es.

MeSH

CalibrationChromatography, GasOlive OilPesticide ResiduesPlant OilsSolid Phase ExtractionSoybean OilSunflower OilTandem Mass SpectrometryTime Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27530421

Citation

Parrilla Vázquez, P, et al. "Large Multiresidue Analysis of Pesticides in Edible Vegetable Oils By Using Efficient Solid-phase Extraction Sorbents Based On Quick, Easy, Cheap, Effective, Rugged and Safe Methodology Followed By Gas Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1463, 2016, pp. 20-31.
Parrilla Vázquez P, Hakme E, Uclés S, et al. Large multiresidue analysis of pesticides in edible vegetable oils by using efficient solid-phase extraction sorbents based on quick, easy, cheap, effective, rugged and safe methodology followed by gas chromatography-tandem mass spectrometry. J Chromatogr A. 2016;1463:20-31.
Parrilla Vázquez, P., Hakme, E., Uclés, S., Cutillas, V., Martínez Galera, M., Mughari, A. R., & Fernández-Alba, A. R. (2016). Large multiresidue analysis of pesticides in edible vegetable oils by using efficient solid-phase extraction sorbents based on quick, easy, cheap, effective, rugged and safe methodology followed by gas chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1463, 20-31. https://doi.org/10.1016/j.chroma.2016.08.008
Parrilla Vázquez P, et al. Large Multiresidue Analysis of Pesticides in Edible Vegetable Oils By Using Efficient Solid-phase Extraction Sorbents Based On Quick, Easy, Cheap, Effective, Rugged and Safe Methodology Followed By Gas Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2016 Sep 9;1463:20-31. PubMed PMID: 27530421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Large multiresidue analysis of pesticides in edible vegetable oils by using efficient solid-phase extraction sorbents based on quick, easy, cheap, effective, rugged and safe methodology followed by gas chromatography-tandem mass spectrometry. AU - Parrilla Vázquez,P, AU - Hakme,E, AU - Uclés,S, AU - Cutillas,V, AU - Martínez Galera,M, AU - Mughari,A R, AU - Fernández-Alba,A R, Y1 - 2016/08/04/ PY - 2016/05/10/received PY - 2016/08/01/revised PY - 2016/08/03/accepted PY - 2016/8/18/entrez PY - 2016/8/18/pubmed PY - 2016/12/15/medline KW - EMR-Lipid KW - Edible vegetable oils KW - GC–MS/MS KW - Multiresidue pesticide analysis KW - Procedural Standard Calibration SP - 20 EP - 31 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1463 N2 - The aim of this research was to adapt the QuEChERS method for routine pesticide multiresidue analysis in edible vegetable oil samples using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). Several clean-up approaches were tested: (a) D-SPE with Enhanced Matrix Removal-Lipid (EMR-Lipid™); (b) D-SPE with PSA; (c) D-SPE with Z-Sep; (d) SPE with Z-Sep. Clean-up methods were evaluated in terms of fat removal from the extracts, recoveries and extraction precision for 213 pesticides in different matrices (soybean, sunflower and extra-virgin olive oil). The QuEChERS protocol with EMR-Lipid d-SPE provided the best reduction of co-extracted matrix compounds with the highest number of pesticides exhibiting mean recoveries in the 70-120% range, and the lowest relative standard deviations values (4% on average). A simple and rapid (only 5min) freeze-out step with dry ice (CO2 at -76°C) prior to d-SPE clean-up ensured much better removal of co-extracted matrix compounds in compliance of the necessity in routine analysis. Procedural Standard Calibration was established in order to compensate for recovery losses of certain pesticides and possible matrix effects. Limits of quantification were 10μgkg(-1) for the majority of the pesticides. The modified methodology was applied for the analysis of different 17 oil samples. Fourteen pesticides were detected with values lower than MRLs and their concentration ranged between 10.2 and 156.0μgkg(-1). SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/27530421/Large_multiresidue_analysis_of_pesticides_in_edible_vegetable_oils_by_using_efficient_solid_phase_extraction_sorbents_based_on_quick_easy_cheap_effective_rugged_and_safe_methodology_followed_by_gas_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓10]

Related Citations

More