Tags

Type your tag names separated by a space and hit enter

Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2016 Nov; 129(11):2179-2190.TA

Abstract

KEY MESSAGE

Nine QTL for black point resistance in wheat were identified using a RIL population derived from a Linmai 2/Zhong 892 cross and 90K SNP assay. Black point, discoloration of the embryo end of the grain, downgrades wheat grain quality leading to significant economic losses to the wheat industry. The availability of molecular markers will accelerate improvement of black point resistance in wheat breeding. The aims of this study were to identify quantitative trait loci (QTL) for black point resistance and tightly linked molecular markers, and to search for candidate genes using a high-density genetic linkage map of wheat. A recombinant inbred line (RIL) population derived from the cross Linmai 2/Zhong 892 was evaluated for black point reaction during the 2011-2012, 2012-2013 and 2013-2014 cropping seasons, providing data for seven environments. A high-density linkage map was constructed by genotyping the RILs with the wheat 90K single nucleotide polymorphism (SNP) chip. Composite interval mapping detected nine QTL on chromosomes 2AL, 2BL, 3AL, 3BL, 5AS, 6A, 7AL (2) and 7BS, designated as QBp.caas-2AL, QBp.caas-2BL, QBp.caas-3AL, QBp.caas-3BL, QBp.caas-5AS, QBp.caas-6A, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS, respectively. All resistance alleles, except for QBp.caas-7AL.1 from Linmai 2, were contributed by Zhong 892. QBp.caas-3BL, QBp.caas-5AS, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS probably represent new loci for black point resistance. Sequences of tightly linked SNPs were used to survey wheat and related cereal genomes identifying three candidate genes for black point resistance. The tightly linked SNP markers can be used in marker-assisted breeding in combination with the kompetitive allele specific PCR technique to improve black point resistance.

Authors+Show Affiliations

Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. Department of Plant Genetics and Breeding, State Key Laboratory for Agrobiotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100193, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing, 100081, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, 20 Jingjusi Road, Chengdu, 610066, Sichuan, China.Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, Gansu, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China.Department of Plant Genetics and Breeding, State Key Laboratory for Agrobiotechnology, China Agricultural University, 2 Yuanmingyuan West Road, Beijing, 100193, China.Institute of Crop Science, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing, 100081, China. xiaxianchun@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27531362

Citation

Liu, Jindong, et al. "Genome-wide Linkage Mapping of QTL for Black Point Reaction in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 129, no. 11, 2016, pp. 2179-2190.
Liu J, He Z, Wu L, et al. Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2016;129(11):2179-2190.
Liu, J., He, Z., Wu, L., Bai, B., Wen, W., Xie, C., & Xia, X. (2016). Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 129(11), 2179-2190.
Liu J, et al. Genome-wide Linkage Mapping of QTL for Black Point Reaction in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2016;129(11):2179-2190. PubMed PMID: 27531362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide linkage mapping of QTL for black point reaction in bread wheat (Triticum aestivum L.). AU - Liu,Jindong, AU - He,Zhonghu, AU - Wu,Ling, AU - Bai,Bin, AU - Wen,Weie, AU - Xie,Chaojie, AU - Xia,Xianchun, Y1 - 2016/08/16/ PY - 2016/05/06/received PY - 2016/07/30/accepted PY - 2016/10/21/pubmed PY - 2017/2/7/medline PY - 2016/8/18/entrez SP - 2179 EP - 2190 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 129 IS - 11 N2 - KEY MESSAGE: Nine QTL for black point resistance in wheat were identified using a RIL population derived from a Linmai 2/Zhong 892 cross and 90K SNP assay. Black point, discoloration of the embryo end of the grain, downgrades wheat grain quality leading to significant economic losses to the wheat industry. The availability of molecular markers will accelerate improvement of black point resistance in wheat breeding. The aims of this study were to identify quantitative trait loci (QTL) for black point resistance and tightly linked molecular markers, and to search for candidate genes using a high-density genetic linkage map of wheat. A recombinant inbred line (RIL) population derived from the cross Linmai 2/Zhong 892 was evaluated for black point reaction during the 2011-2012, 2012-2013 and 2013-2014 cropping seasons, providing data for seven environments. A high-density linkage map was constructed by genotyping the RILs with the wheat 90K single nucleotide polymorphism (SNP) chip. Composite interval mapping detected nine QTL on chromosomes 2AL, 2BL, 3AL, 3BL, 5AS, 6A, 7AL (2) and 7BS, designated as QBp.caas-2AL, QBp.caas-2BL, QBp.caas-3AL, QBp.caas-3BL, QBp.caas-5AS, QBp.caas-6A, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS, respectively. All resistance alleles, except for QBp.caas-7AL.1 from Linmai 2, were contributed by Zhong 892. QBp.caas-3BL, QBp.caas-5AS, QBp.caas-7AL.1, QBp.caas-7AL.2 and QBp.caas-7BS probably represent new loci for black point resistance. Sequences of tightly linked SNPs were used to survey wheat and related cereal genomes identifying three candidate genes for black point resistance. The tightly linked SNP markers can be used in marker-assisted breeding in combination with the kompetitive allele specific PCR technique to improve black point resistance. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27531362/Genome_wide_linkage_mapping_of_QTL_for_black_point_reaction_in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-016-2766-3 DB - PRIME DP - Unbound Medicine ER -