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Long-term stability of cannabinoids in oral fluid after controlled cannabis administration.
Drug Test Anal. 2017 Jan; 9(1):143-147.DT

Abstract

Cannabinoid stability in oral fluid (OF) is important for assuring accurate results since OF has become a valid alternative matrix of choice for drug testing. We previously published OF cannabinoid stability studies using Quantisal™, Oral-Eze®, and StatSure™ devices stored at room temperature for 1 week, 4 °C for up to 4 weeks, and at -20 °C up to 24 weeks. Extending refrigerated stability up to 3 months would be helpful for clinical and forensic testing, for re-analysis of OF samples and for batching research analyses. Individual authentic OF pools were prepared after controlled smoking of a 6.9% ∆9 -tetrahydracannabinol cannabis cigarette; the Quantisal™ device was utilized for OF collection. Fifteen healthy volunteers participated in the Institutional Review Board-approved study. Stability for THC, 11-nor-9-carboxy-THC (THCCOOH), ∆9 -tetrahydrocannabivarin (THCV), cannabidiol (CBD), and cannabigerol (CBG) were determined after storage at 4 °C for 1, 2, and 3 months. Results within ±20% of baseline concentrations were considered stable. All analytes were stable for up to 2 months at 4 °C for all participants with positive baseline concentrations. Baseline concentrations were highly variable. In total, THC, THCCOOH, THCV, CBD, and CBG were stable for 3 months at 4 °C for pooled positive specimens from 14 of 15, 8 of 9, 7 of 8, 8 of 9, and 9 of 10 participants, respectively. In conclusion, Quantisal™-collected OF specimens should be stored at 4 °C for no more than two months to assure accurate THC, THCCOOH, THCV, CBD, and CBG quantitative results; only one participant's OF was unstable at three months. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

Authors+Show Affiliations

Chemistry and Drug Metabolism Section, Clinical Pharmacology and Therapeutics Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.Chemistry and Drug Metabolism Section, Clinical Pharmacology and Therapeutics Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.Chemistry and Drug Metabolism Section, Clinical Pharmacology and Therapeutics Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.Chemistry and Drug Metabolism Section, Clinical Pharmacology and Therapeutics Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA.Chemistry and Drug Metabolism Section, Clinical Pharmacology and Therapeutics Research Branch, Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224, USA. University of Maryland School of Medicine, Baltimore, MD 21201.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27539096

Citation

Scheidweiler, Karl B., et al. "Long-term Stability of Cannabinoids in Oral Fluid After Controlled Cannabis Administration." Drug Testing and Analysis, vol. 9, no. 1, 2017, pp. 143-147.
Scheidweiler KB, Andersson M, Swortwood MJ, et al. Long-term stability of cannabinoids in oral fluid after controlled cannabis administration. Drug Test Anal. 2017;9(1):143-147.
Scheidweiler, K. B., Andersson, M., Swortwood, M. J., Sempio, C., & Huestis, M. A. (2017). Long-term stability of cannabinoids in oral fluid after controlled cannabis administration. Drug Testing and Analysis, 9(1), 143-147. https://doi.org/10.1002/dta.2056
Scheidweiler KB, et al. Long-term Stability of Cannabinoids in Oral Fluid After Controlled Cannabis Administration. Drug Test Anal. 2017;9(1):143-147. PubMed PMID: 27539096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term stability of cannabinoids in oral fluid after controlled cannabis administration. AU - Scheidweiler,Karl B, AU - Andersson,Maria, AU - Swortwood,Madeleine J, AU - Sempio,Cristina, AU - Huestis,Marilyn A, Y1 - 2016/09/13/ PY - 2016/05/20/received PY - 2016/08/15/revised PY - 2016/08/17/accepted PY - 2016/8/20/pubmed PY - 2017/5/17/medline PY - 2016/8/20/entrez KW - cannabinoid KW - liquid chromatography-mass spectrometry KW - oral fluid KW - stability SP - 143 EP - 147 JF - Drug testing and analysis JO - Drug Test Anal VL - 9 IS - 1 N2 - Cannabinoid stability in oral fluid (OF) is important for assuring accurate results since OF has become a valid alternative matrix of choice for drug testing. We previously published OF cannabinoid stability studies using Quantisal™, Oral-Eze®, and StatSure™ devices stored at room temperature for 1 week, 4 °C for up to 4 weeks, and at -20 °C up to 24 weeks. Extending refrigerated stability up to 3 months would be helpful for clinical and forensic testing, for re-analysis of OF samples and for batching research analyses. Individual authentic OF pools were prepared after controlled smoking of a 6.9% ∆9 -tetrahydracannabinol cannabis cigarette; the Quantisal™ device was utilized for OF collection. Fifteen healthy volunteers participated in the Institutional Review Board-approved study. Stability for THC, 11-nor-9-carboxy-THC (THCCOOH), ∆9 -tetrahydrocannabivarin (THCV), cannabidiol (CBD), and cannabigerol (CBG) were determined after storage at 4 °C for 1, 2, and 3 months. Results within ±20% of baseline concentrations were considered stable. All analytes were stable for up to 2 months at 4 °C for all participants with positive baseline concentrations. Baseline concentrations were highly variable. In total, THC, THCCOOH, THCV, CBD, and CBG were stable for 3 months at 4 °C for pooled positive specimens from 14 of 15, 8 of 9, 7 of 8, 8 of 9, and 9 of 10 participants, respectively. In conclusion, Quantisal™-collected OF specimens should be stored at 4 °C for no more than two months to assure accurate THC, THCCOOH, THCV, CBD, and CBG quantitative results; only one participant's OF was unstable at three months. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/27539096/Long_term_stability_of_cannabinoids_in_oral_fluid_after_controlled_cannabis_administration_ L2 - https://doi.org/10.1002/dta.2056 DB - PRIME DP - Unbound Medicine ER -