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Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors.
Plant Biotechnol J. 2017 03; 15(3):306-317.PB

Abstract

As a key virulence strategy to cause bacterial leaf blight, Xanthomonas oryzae pv. oryzae (Xoo) injects into the plant cell DNA-binding proteins called transcription activator-like effectors (TALEs) that bind to effector-binding elements (EBEs) in a sequence-specific manner, resulting in host gene induction. TALEs AvrXa7, PthXo3, TalC and Tal5, found in geographically distant Xoo strains, all target OsSWEET14, thus considered as a pivotal TALE target acting as major susceptibility factor during rice-Xoo interactions. Here, we report the generation of an allele library of the OsSWEET14 promoter through stable expression of TALE-nuclease (TALEN) constructs in rice. The susceptibility level of lines carrying mutations in AvrXa7, Tal5 or TalC EBEs was assessed. Plants edited in AvrXa7 or Tal5 EBEs were resistant to bacterial strains relying on the corresponding TALE. Surprisingly, although indels within TalC EBE prevented OsSWEET14 induction in response to BAI3 wild-type bacteria relying on TalC, loss of TalC responsiveness failed to confer resistance to this strain. The TalC EBE mutant line was, however, resistant to a strain expressing an artificial SWEET14-inducing TALE whose EBE was also edited in this line. This work offers the first set of alleles edited in TalC EBE and uncovers a distinct, broader range of activities for TalC compared to AvrXa7 or Tal5. We propose the existence of additional targets for TalC beyond SWEET14, suggesting that TALE-mediated plant susceptibility may result from induction of several, genetically redundant, host susceptibility genes by a single effector.

Authors+Show Affiliations

UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.Institut für Biologie, Institutsbereich Genetik, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.Institut für Biologie, Institutsbereich Genetik, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.CIRAD, UMR AGAP (Amélioration génétique et Adaptation des Plantes), Montpellier, France.CIRAD, UMR AGAP (Amélioration génétique et Adaptation des Plantes), Montpellier, France.UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.CIRAD, UMR AGAP (Amélioration génétique et Adaptation des Plantes), Montpellier, France.Institut für Biologie, Institutsbereich Genetik, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany.UMR Interactions Plantes Microorganismes Environnement (IPME), IRD-CIRAD-Université, Montpellier, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27539813

Citation

Blanvillain-Baufumé, Servane, et al. "Targeted Promoter Editing for Rice Resistance to Xanthomonas Oryzae Pv. Oryzae Reveals Differential Activities for SWEET14-inducing TAL Effectors." Plant Biotechnology Journal, vol. 15, no. 3, 2017, pp. 306-317.
Blanvillain-Baufumé S, Reschke M, Solé M, et al. Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors. Plant Biotechnol J. 2017;15(3):306-317.
Blanvillain-Baufumé, S., Reschke, M., Solé, M., Auguy, F., Doucoure, H., Szurek, B., Meynard, D., Portefaix, M., Cunnac, S., Guiderdoni, E., Boch, J., & Koebnik, R. (2017). Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors. Plant Biotechnology Journal, 15(3), 306-317. https://doi.org/10.1111/pbi.12613
Blanvillain-Baufumé S, et al. Targeted Promoter Editing for Rice Resistance to Xanthomonas Oryzae Pv. Oryzae Reveals Differential Activities for SWEET14-inducing TAL Effectors. Plant Biotechnol J. 2017;15(3):306-317. PubMed PMID: 27539813.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14-inducing TAL effectors. AU - Blanvillain-Baufumé,Servane, AU - Reschke,Maik, AU - Solé,Montserrat, AU - Auguy,Florence, AU - Doucoure,Hinda, AU - Szurek,Boris, AU - Meynard,Donaldo, AU - Portefaix,Murielle, AU - Cunnac,Sébastien, AU - Guiderdoni,Emmanuel, AU - Boch,Jens, AU - Koebnik,Ralf, Y1 - 2016/12/17/ PY - 2016/02/12/received PY - 2016/08/12/revised PY - 2016/08/12/accepted PY - 2016/8/20/pubmed PY - 2017/10/21/medline PY - 2016/8/20/entrez KW - TALEN KW - bacterial leaf blight KW - genome engineering KW - susceptibility gene KW - transgene-free plants SP - 306 EP - 317 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 15 IS - 3 N2 - As a key virulence strategy to cause bacterial leaf blight, Xanthomonas oryzae pv. oryzae (Xoo) injects into the plant cell DNA-binding proteins called transcription activator-like effectors (TALEs) that bind to effector-binding elements (EBEs) in a sequence-specific manner, resulting in host gene induction. TALEs AvrXa7, PthXo3, TalC and Tal5, found in geographically distant Xoo strains, all target OsSWEET14, thus considered as a pivotal TALE target acting as major susceptibility factor during rice-Xoo interactions. Here, we report the generation of an allele library of the OsSWEET14 promoter through stable expression of TALE-nuclease (TALEN) constructs in rice. The susceptibility level of lines carrying mutations in AvrXa7, Tal5 or TalC EBEs was assessed. Plants edited in AvrXa7 or Tal5 EBEs were resistant to bacterial strains relying on the corresponding TALE. Surprisingly, although indels within TalC EBE prevented OsSWEET14 induction in response to BAI3 wild-type bacteria relying on TalC, loss of TalC responsiveness failed to confer resistance to this strain. The TalC EBE mutant line was, however, resistant to a strain expressing an artificial SWEET14-inducing TALE whose EBE was also edited in this line. This work offers the first set of alleles edited in TalC EBE and uncovers a distinct, broader range of activities for TalC compared to AvrXa7 or Tal5. We propose the existence of additional targets for TalC beyond SWEET14, suggesting that TALE-mediated plant susceptibility may result from induction of several, genetically redundant, host susceptibility genes by a single effector. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/27539813/Targeted_promoter_editing_for_rice_resistance_to_Xanthomonas_oryzae_pv__oryzae_reveals_differential_activities_for_SWEET14_inducing_TAL_effectors_ L2 - https://doi.org/10.1111/pbi.12613 DB - PRIME DP - Unbound Medicine ER -