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Effects of a silver nanomaterial on cellular organelles and time course of oxidative stress in a fish cell line (PLHC-1).

Abstract

Among the nanomaterials currently in commercial products, those based on silver are the most used, and so there is a high probability that silver nanoparticles (AgNPs) will be released into aquatic environments where they could adversely affect aquatic organisms, including fish. Taking this into account, the aim of the present work was to characterize in depth the mechanisms underlying the toxic action of AgNPs using fish cell lines, determining specifically the contribution of alterations in cellular structures and oxidative stress time course to the cytotoxicity of AgNPs. Since liver plays a key role in detoxification, the hepatoma cell line PLHC-1 was used. Exposure to AgNPs (NM-300K, obtained from the Joint Research Centre Repository) caused alterations at the lysosomal and mitochondrial levels at lower concentrations than those that disrupted plasma membrane (evaluated by means of neutral red, alamarBlue, and 5-carboxyfluorescein diacetate, acetoxymethyl ester assays respectively). AgNO3, used as a control Ag+ ion source, produced similar cytotoxic effects but at lower concentrations than AgNPs. Both silver forms caused oxidative disruption but the initial response was delayed in AgNPs until 6h of exposure. Transmission electron microscopy analysis also evidenced the disruption of mitochondrial structures in cells exposed to cytotoxic concentrations of both forms of silver. At non-cytotoxic concentrations, AgNPs were detected inside the nucleoli and mitochondria, thereby pointing to long-term effects. The present work evidences the mutual interaction between the induction of oxidative stress and the alterations of cellular structures, particularly mitochondria, as cytotoxicity mechanisms not exclusively associated to NPs.

Authors+Show Affiliations

Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña, Km 7.5, Madrid, Spain. Electronic address: bermejo.azucena@inia.es.Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Avenida. Complutense 22, E-28040 Madrid, Spain.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña, Km 7.5, Madrid, Spain.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Medio Ambiente, Carretera de la Coruña, Km 7.5, Madrid, Spain. Electronic address: jmnavas@inia.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27544301

Citation

Bermejo-Nogales, A, et al. "Effects of a Silver Nanomaterial On Cellular Organelles and Time Course of Oxidative Stress in a Fish Cell Line (PLHC-1)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 190, 2016, pp. 54-65.
Bermejo-Nogales A, Fernández M, Fernández-Cruz ML, et al. Effects of a silver nanomaterial on cellular organelles and time course of oxidative stress in a fish cell line (PLHC-1). Comp Biochem Physiol C Toxicol Pharmacol. 2016;190:54-65.
Bermejo-Nogales, A., Fernández, M., Fernández-Cruz, M. L., & Navas, J. M. (2016). Effects of a silver nanomaterial on cellular organelles and time course of oxidative stress in a fish cell line (PLHC-1). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 190, 54-65. https://doi.org/10.1016/j.cbpc.2016.08.004
Bermejo-Nogales A, et al. Effects of a Silver Nanomaterial On Cellular Organelles and Time Course of Oxidative Stress in a Fish Cell Line (PLHC-1). Comp Biochem Physiol C Toxicol Pharmacol. 2016;190:54-65. PubMed PMID: 27544301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of a silver nanomaterial on cellular organelles and time course of oxidative stress in a fish cell line (PLHC-1). AU - Bermejo-Nogales,A, AU - Fernández,M, AU - Fernández-Cruz,M L, AU - Navas,J M, Y1 - 2016/08/17/ PY - 2016/06/09/received PY - 2016/07/29/revised PY - 2016/08/12/accepted PY - 2016/8/22/pubmed PY - 2017/2/14/medline PY - 2016/8/22/entrez KW - Cell line KW - Fish KW - Mitochondria KW - Oxidative stress KW - ROS KW - Silver nanoparticle SP - 54 EP - 65 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 190 N2 - Among the nanomaterials currently in commercial products, those based on silver are the most used, and so there is a high probability that silver nanoparticles (AgNPs) will be released into aquatic environments where they could adversely affect aquatic organisms, including fish. Taking this into account, the aim of the present work was to characterize in depth the mechanisms underlying the toxic action of AgNPs using fish cell lines, determining specifically the contribution of alterations in cellular structures and oxidative stress time course to the cytotoxicity of AgNPs. Since liver plays a key role in detoxification, the hepatoma cell line PLHC-1 was used. Exposure to AgNPs (NM-300K, obtained from the Joint Research Centre Repository) caused alterations at the lysosomal and mitochondrial levels at lower concentrations than those that disrupted plasma membrane (evaluated by means of neutral red, alamarBlue, and 5-carboxyfluorescein diacetate, acetoxymethyl ester assays respectively). AgNO3, used as a control Ag+ ion source, produced similar cytotoxic effects but at lower concentrations than AgNPs. Both silver forms caused oxidative disruption but the initial response was delayed in AgNPs until 6h of exposure. Transmission electron microscopy analysis also evidenced the disruption of mitochondrial structures in cells exposed to cytotoxic concentrations of both forms of silver. At non-cytotoxic concentrations, AgNPs were detected inside the nucleoli and mitochondria, thereby pointing to long-term effects. The present work evidences the mutual interaction between the induction of oxidative stress and the alterations of cellular structures, particularly mitochondria, as cytotoxicity mechanisms not exclusively associated to NPs. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/27544301/Effects_of_a_silver_nanomaterial_on_cellular_organelles_and_time_course_of_oxidative_stress_in_a_fish_cell_line__PLHC_1__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(16)30093-X DB - PRIME DP - Unbound Medicine ER -