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Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis.
BMC Vet Res. 2016 Aug 20; 12(1):172.BV

Abstract

BACKGROUND

Hereditary bisalbuminemia is a relatively rare anomaly characterized by the occurrence of two albumin fractions on serum protein separation by electrophoresis. In human medicine, it is usually revealed by chance, is not been clearly associated with a specific disease and the causative genetic alteration is a point mutation of human serum albumin gene inherited in an autosomal codominant pattern. This type of alteration is well recognizable by capillary zone electrophoresis (CZE), whilst agarose gel electrophoresis (AGE) not always produces a clear separation of albumin fractions. The aims of this study is to report the presence of this abnormality in two separate groups of related bottlenose dolphins and to compare the results obtained with capillary zone and agarose gel electrophoresis.

RESULTS

Serum samples from 40 bottlenose dolphins kept under human care were analyzed. In 9 samples a double albumin peak was evident in CZE electrophoresis while no double peak was noted in AGE profile. Since only an apparently wider albumin peaks were noted in some AGE electrophoretic profiles, the ratio between base and height (b/h) of the albumin peak was calculated and each point-value recorded in the whole set of data was used to calculate a receiver operating characteristic curve: when the b/h ratio of albumin peak was equal or higher than 0.25, the sensitivity and specificity of AGE to detect bisalbuminemic samples were 87 and 63 %, respectively. The bisalbuminemic dolphins belong to two distinct families: in the first family, all the siblings derived from the same normal sire were bisalbuminemic, whereas in the second family bisalbuminemia was present in a sire and in two out of three siblings.

CONCLUSIONS

We report for the first time the presence of hereditary bisalbuminemia in two groups of related bottlenose dolphins identified by means of CZE and we confirm that AGE could fail in the identification of this alteration.

Authors+Show Affiliations

Costa Edutainment spa, Acquario di Genova, Area Porto Antico, Ponte Spinola, 16128, Genoa, Italy.Department of Comparative Biomedicine and Food Science, University of Padua, AGRIPOLIS - Viale dell'Università 16, 35020, Agripolis, Legnaro, PD, Italy.Costa Edutainment spa, Acquario di Genova, Area Porto Antico, Ponte Spinola, 16128, Genoa, Italy.Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133, Milan, Italy.Costa Edutainment spa, Acquario di Genova, Area Porto Antico, Ponte Spinola, 16128, Genoa, Italy.Department of Comparative Biomedicine and Food Science, University of Padua, AGRIPOLIS - Viale dell'Università 16, 35020, Agripolis, Legnaro, PD, Italy.Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133, Milan, Italy.Costa Edutainment spa, Acquario di Genova, Area Porto Antico, Ponte Spinola, 16128, Genoa, Italy.Department of Comparative Biomedicine and Food Science, University of Padua, AGRIPOLIS - Viale dell'Università 16, 35020, Agripolis, Legnaro, PD, Italy. mariaelena.gelain@unipd.it.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27544582

Citation

Gili, Claudia, et al. "Detection of Hereditary Bisalbuminemia in Bottlenose Dolphins (Tursiops Truncatus, Montagu 1821): Comparison Between Capillary Zone and Agarose Gel Electrophoresis." BMC Veterinary Research, vol. 12, no. 1, 2016, p. 172.
Gili C, Bonsembiante F, Bonanni R, et al. Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis. BMC Vet Res. 2016;12(1):172.
Gili, C., Bonsembiante, F., Bonanni, R., Giordano, A., Ledda, S., Beffagna, G., Paltrinieri, S., Sommer, M., & Gelain, M. E. (2016). Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis. BMC Veterinary Research, 12(1), 172. https://doi.org/10.1186/s12917-016-0801-x
Gili C, et al. Detection of Hereditary Bisalbuminemia in Bottlenose Dolphins (Tursiops Truncatus, Montagu 1821): Comparison Between Capillary Zone and Agarose Gel Electrophoresis. BMC Vet Res. 2016 Aug 20;12(1):172. PubMed PMID: 27544582.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of hereditary bisalbuminemia in bottlenose dolphins (Tursiops truncatus, Montagu 1821): comparison between capillary zone and agarose gel electrophoresis. AU - Gili,Claudia, AU - Bonsembiante,Federico, AU - Bonanni,Renzo, AU - Giordano,Alessia, AU - Ledda,Sabina, AU - Beffagna,Giorgia, AU - Paltrinieri,Saverio, AU - Sommer,Matteo, AU - Gelain,Maria Elena, Y1 - 2016/08/20/ PY - 2015/11/24/received PY - 2016/08/12/accepted PY - 2016/8/22/entrez PY - 2016/8/22/pubmed PY - 2016/12/31/medline KW - Agarose gel electrophoresis KW - Bisalbuminemia KW - Capillary zone electrophoresis KW - Tursiops truncatus SP - 172 EP - 172 JF - BMC veterinary research JO - BMC Vet. Res. VL - 12 IS - 1 N2 - BACKGROUND: Hereditary bisalbuminemia is a relatively rare anomaly characterized by the occurrence of two albumin fractions on serum protein separation by electrophoresis. In human medicine, it is usually revealed by chance, is not been clearly associated with a specific disease and the causative genetic alteration is a point mutation of human serum albumin gene inherited in an autosomal codominant pattern. This type of alteration is well recognizable by capillary zone electrophoresis (CZE), whilst agarose gel electrophoresis (AGE) not always produces a clear separation of albumin fractions. The aims of this study is to report the presence of this abnormality in two separate groups of related bottlenose dolphins and to compare the results obtained with capillary zone and agarose gel electrophoresis. RESULTS: Serum samples from 40 bottlenose dolphins kept under human care were analyzed. In 9 samples a double albumin peak was evident in CZE electrophoresis while no double peak was noted in AGE profile. Since only an apparently wider albumin peaks were noted in some AGE electrophoretic profiles, the ratio between base and height (b/h) of the albumin peak was calculated and each point-value recorded in the whole set of data was used to calculate a receiver operating characteristic curve: when the b/h ratio of albumin peak was equal or higher than 0.25, the sensitivity and specificity of AGE to detect bisalbuminemic samples were 87 and 63 %, respectively. The bisalbuminemic dolphins belong to two distinct families: in the first family, all the siblings derived from the same normal sire were bisalbuminemic, whereas in the second family bisalbuminemia was present in a sire and in two out of three siblings. CONCLUSIONS: We report for the first time the presence of hereditary bisalbuminemia in two groups of related bottlenose dolphins identified by means of CZE and we confirm that AGE could fail in the identification of this alteration. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/27544582/Detection_of_hereditary_bisalbuminemia_in_bottlenose_dolphins__Tursiops_truncatus_Montagu_1821_:_comparison_between_capillary_zone_and_agarose_gel_electrophoresis_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0801-x DB - PRIME DP - Unbound Medicine ER -